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    Phenyl Xanthene Dyes
    4.
    发明申请
    Phenyl Xanthene Dyes 审中-公开
    苯基呫吨染料

    公开(公告)号：US20130171715A1

    公开(公告)日：2013-07-04

    申请号：US13742667

    申请日：2013-01-16

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Joe Y.L. Lam ,  Steven M. Menchen ,  Ruiming Zou ,  Scott C. Benson

    IPC分类号： C09B11/12 ,  C09B11/06 ,  C09B11/26

    CPC分类号： C09B11/24 ,  C07D311/82 ,  C07D491/14 ,  C09B11/06 ,  C09B11/12 ,  C09B11/22 ,  C09B11/26 ,  C09B13/00 ,  C09B57/00 ,  C09B62/0025

    摘要： Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes. The phenyl xanthene dyes are useful for a number of purposes, including labels for use in automated DNA sequencing as well the formation of fluorescent “bar codes” for polymeric particles used in the multiplexed analysis of analytes.

    摘要翻译： 描述了包含任何含有特定C 9苯环的任何荧光素，罗丹明或若丹明的荧光苯基呫吨染料。 下C9苯环上的一个或两个邻基被选自烷基，杂烷基，烷氧基，卤素，卤代烷基，氨基，巯基，烷硫基，氰基，异氰基，氰基，巯基氰基，亚硝基，硝基，叠氮基 ，磺酰亚胺基，亚磺酰基和亚磺酰基。 在一个实施方案中，使用卤素和/或羟基。 最佳染料含有较低的C 9苯环，其中两个邻基团相同，并且下环表现出相对于从苯环连接点到呫吨染料的其余部分的假想轴的对称性。 附件点。 苯基呫吨染料可以被活化。 此外，苯基呫吨染料可以与一种或多种包括其它染料的物质共轭。 苯基呫吨染料可用于许多目的，包括用于自动DNA测序的标记，以及用于分析物复用分析中使用的聚合物颗粒的荧光“条形码”。

    Methods and systems for point of use removal of sacrificial material
    7.
    发明授权
    Methods and systems for point of use removal of sacrificial material 有权

    公开(公告)号：US09852919B2

    公开(公告)日：2017-12-26

    申请号：US14676697

    申请日：2015-04-01

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Christina E. Inman ,  Alexander Mastroianni ,  Wolfgang Hinz ,  Shifeng Li ,  Scott C. Benson

    IPC分类号： H01L21/00 ,  H01L21/311 ,  G01N27/414

    CPC分类号： H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

    摘要： A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

    DDAO Compounds as Fluorescent Reference Standards
    9.
    发明申请
    DDAO Compounds as Fluorescent Reference Standards 有权
    DDAO化合物作为荧光参考标准

    公开(公告)号：US20140134615A1

    公开(公告)日：2014-05-15

    申请号：US14071552

    申请日：2013-11-04

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Scott C. Benson ,  Cinna Monighetti ,  Sandy M. Koepf

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C09B15/00 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2545/101 ,  C12Q2561/113 ,  C12Q2563/107

    摘要： According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I); In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (I), a buffer, a selection of nucleotides and a protein.

    摘要翻译： 根据本教导，提供利用至少一种式（I）的参考染料的方法和组合物; 在一些实施方案中，一种方法包括测量报道染料和至少一种式（I）的参考染料的检测信号。 在一些实施方案中，组合物包含式（I）的参考染料，缓冲液，核苷酸和蛋白质的选择。