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    DUAL LABELING METHODS FOR MEASURING CELLULAR PROLIFERATION
    1.
    发明申请
    DUAL LABELING METHODS FOR MEASURING CELLULAR PROLIFERATION 审中-公开
    用于测量细胞增殖的双标记方法

    公开(公告)号：US20110118142A1

    公开(公告)日：2011-05-19

    申请号：US12993079

    申请日：2009-05-14

    Applicant: Scott Clarke ,  Jolene Bradford

    Inventor： Scott Clarke ,  Jolene Bradford

    IPC: C40B30/06 ,  C12Q1/68

    CPC classification number: C12Q1/6809 ,  C12Q1/6804 ,  C12Q1/6846 ,  C12Q2565/1025 ,  C12Q2525/101

    Abstract: The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

    Abstract translation: 本发明提供了一种通过核酸双重脉冲标记来测量细胞新生核酸合成的方法。 具有核苷类似物的核酸的第一次脉冲标记允许建立基线核酸合成速率。 用第二核苷类似物脉冲标记核酸，然后允许测量核酸合成的任何变化。 核酸合成可以作为细胞增殖，DNA或基因表达，RNA来测量。 该方法不需要在脉冲标签之间产生潜在的伪影中介洗涤步骤。 此外，该方法可用于通过用竞争性核苷类似物在治疗前或之前用受试化合物处理细胞或生物体来筛选化合物对细胞增殖的影响。

    Acoustic Cytometry Methods and Protocols
    4.
    发明申请
    Acoustic Cytometry Methods and Protocols 审中-公开
    声学细胞计数法和方案

    公开(公告)号：US20140147860A1

    公开(公告)日：2014-05-29

    申请号：US14129777

    申请日：2012-06-27

    Applicant: Gregory Kaduchak ,  Michael Ward ,  Jolene Bradford ,  Bradley Dubbels ,  Barbara Seredick ,  Rickie Kerndt ,  Kristi Haataja ,  April Anderson ,  Penny Melquist ,  Christopher Langsdorf ,  Justin Hicks ,  Kathleen Kihn

    Inventor： Gregory Kaduchak ,  Michael Ward ,  Jolene Bradford ,  Bradley Dubbels ,  Barbara Seredick ,  Rickie Kerndt ,  Kristi Haataja ,  April Anderson ,  Penny Melquist ,  Christopher Langsdorf ,  Justin Hicks ,  Kathleen Kihn

    IPC: G01N33/569

    CPC classification number: G01N33/56966 ,  G01N15/1404 ,  G01N21/6408 ,  G01N21/6486 ,  G01N2015/142

    Abstract: Various embodiments disclosed herein comprise acoustic cytometry based methods, kits, computer software methods and systems to analyze a variety of bioparticles. In one embodiment, a method for analyzing bioparticles comprises: acoustically focusing one or more bioparticles through an interrogation zone; optically exciting the one or more bioparticles in the interrogation zone with an excitation source; detecting an optical signal from the bioparticles; and analyzing the optical signal to characterize at least one quality or quantity parameter of the bioparticles. Properties of biomolecules that may be analyzed include but are not limited to cell proliferation analysis, live/dead cell discrimination, cell cycle analysis, basic phenotyping, immunophenotyping, rare-event detection, apoptosis, phagocytosis, pinocytosis, detection of phosphoproteins, detection of one or more cellular markers, detection of one or more intracellular marker, detection of cancer cells, detection of pathological markers on a cell, microbial cell analysis and/or picophytoplankton analysis.

    Abstract translation: 本文公开的各种实施方案包括基于声学细胞计算的方法，试剂盒，计算机软件方法和用于分析各种生物颗粒的系统。 在一个实施方案中，用于分析生物颗粒的方法包括：通过询问区将一种或多种生物颗粒声学聚焦; 用激发源光学激发询问区中的一个或多个生物颗粒; 从生物颗粒检测光信号; 以及分析所述光学信号以表征所述生物颗粒的至少一个质量或数量参数。 可以分析的生物分子的性质包括但不限于细胞增殖分析，活/死细胞鉴别，细胞周期分析，基本表型，免疫分型，罕见事件检测，细胞凋亡，吞噬作用，胞饮作用，磷蛋白检测，检测一种 或更多的细胞标志物，一种或多种细胞内标志物的检测，癌细胞的检测，细胞上的病理标记的检测，微生物细胞分析和/或浮游生物分析。

    Modified actinomycin-based nucleic acid stains and methods of their use
    8.
    发明授权
    Modified actinomycin-based nucleic acid stains and methods of their use 有权
    改良的基于放线菌素的核酸污渍及其使用方法

    公开(公告)号：US08653261B2

    公开(公告)日：2014-02-18

    申请号：US13126150

    申请日：2009-10-26

    Applicant: Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

    Inventor： Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

    IPC: C07D265/38

    CPC classification number: C07D265/38

    Abstract: Actinomysin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided. The actinomysin-based near IR emitting compounds have the structure: wherein R is H or NH2; R1, R2, R3, and R4 are independently a moiety comprising 1-30 atoms selected from H, O, C, and N, wherein the atoms are in a linear, branched, or cyclic configuration; R3 and/or R4 comprise a quaternary nitrogen atom; and R5 is H, F, or Cl.

    Abstract translation: 提供了基于放射性肌动蛋白的近红外发射化合物及其用作核酸污渍的方法。 基于放电肌注蛋白的近红外发射化合物具有以下结构：其中R是H或NH 2; R1，R2，R3和R4独立地是包含1-30个选自H，O，C和N的原子的部分，其中原子是直链，支链或环状构型; R3和/或R4包含季氮原子; R5为H，F或Cl。

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