公开(公告)号：US5043062A

公开(公告)日：1991-08-27

申请号：US601106

申请日：1990-10-18

申请人： Marsha D. Bale ,  Richard C. Sutton

发明人： Marsha D. Bale ,  Richard C. Sutton

IPC分类号： B01D15/08 ,  B01J20/32

CPC分类号： B01D15/3804 ,  B01J20/261 ,  B01J20/264 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/286 ,  B01J20/3092 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3246 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3274 ,  B01J20/3293 ,  B01D15/3809 ,  B01D15/3823 ,  B01J2220/445 ,  B01J2220/54 ,  B01J2220/58

摘要： A high performance affinity chromatography separation device comprising:A) a chromatographic column containing:B) a packing material;wherein the packing material is a plurality of non-porous, nonodisperse polymeric particles having i) a particle size in the range of 0.01 to about 5 micrometers and ii) surface reactive groups which are directly or indirectly reactive with free amino groups, sulfhydryl groups, carboxy groups or aldehyde groups of biological ligands is disclosed.

摘要翻译： 一种高性能亲和层析分离装置，包括：A）色谱柱，其含有：B）包装材料; 其中所述包装材料是多个非多孔非分散聚合物颗粒，其具有i）在0.01至约5微米范围内的粒度，和ii）与游离氨基直接或间接反应的表面反应性基团，巯基， 公开了生物配体的羧基或醛基。

公开(公告)号：US5374516A

公开(公告)日：1994-12-20

申请号：US315086

申请日：1989-02-24

申请人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

发明人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/546

CPC分类号： G01N33/54306 ,  G01N33/54313 ,  G01N33/545 ,  Y10T428/2991 ,  Y10T428/2998

摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

摘要翻译： 试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。 聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。 亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。 所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。 固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。

公开(公告)号：US5262297A

公开(公告)日：1993-11-16

申请号：US876672

申请日：1992-04-30

申请人： Richard C. Sutton ,  Susan J. Danielson ,  John B. Findlay ,  Fred. T. Oakes ,  Marsha D. B. Oenick ,  Ignazio S. Ponticello ,  Harold C. Warren, III

发明人： Richard C. Sutton ,  Susan J. Danielson ,  John B. Findlay ,  Fred. T. Oakes ,  Marsha D. B. Oenick ,  Ignazio S. Ponticello ,  Harold C. Warren, III

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/543 ,  G01N33/545 ,  G01N83/545 ,  G01N33/546 ,  G01N33/547

CPC分类号： G01N33/54313 ,  C12Q1/6876 ,  C12Q1/701 ,  C12Q1/703 ,  G01N33/545 ,  Y10S436/804 ,  Y10S436/814 ,  Y10S436/817 ,  Y10S436/818 ,  Y10S436/824

摘要： Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.

摘要翻译： 特异性结合方法用于诊断测定和纯化分离，由此特异性结合捕获试剂由具有高反应性羧基的共聚物制备。 这些基团从聚合物表面延伸，在链中具有8至50个原子的连接基团和两个或更多个亚烷基，亚芳基，亚烷基亚芳基或亚芳基亚烷基。 将这些反应性基团连接到生物活性物质如蛋白质或寡核苷酸上，然后参与诊断测定或纯化分离方法。

公开(公告)号：US5149737A

公开(公告)日：1992-09-22

申请号：US654112

申请日：1991-02-12

申请人： Ignazio S. Ponticello ,  Richard C. Sutton

发明人： Ignazio S. Ponticello ,  Richard C. Sutton

IPC分类号： C07C69/40 ,  C07C69/007 ,  C07C69/42 ,  C07C233/05 ,  C07C233/49 ,  C07C251/24 ,  C07C275/38 ,  C07C323/12 ,  C07C323/41 ,  C07C323/50 ,  C07C323/52 ,  C07C323/56 ,  C08F12/00 ,  C08F12/04 ,  C08F12/14 ,  C08F20/02 ,  C08F20/64 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： C07C69/007 ,  C07C323/12 ,  C07C323/52 ,  C07C323/56 ,  C08F12/14 ,  G01N33/54313

摘要： Certain ethylenically unsaturated polymerizable monomers having a reactive carboxy group are useful for preparing homo- and copolymers for a variety of uses, including diagnostic assays. The polymers can be supplied as latex particles in aqueous compositions. The monomers are represented by the structure: ##STR1## wherein: R is hydrogen, halo or alkyl or 1 to 3 carbon atoms,M is hydrogen, an alkali metal ion or an ammonium ion, andL is a linking group having from 8 to 50 atoms in its linking chain, and comprises two or more divalent hydrocarbon groups connected or terminated with one or more nitrogen, oxygen or sulfur atoms, or with one or more groups containing such atoms in the linking chain, provided L has at least one arylene which is not directly connected to the terminal ##STR2## and further provided that none of the hydrocarbon groups has non-aromatic unsaturation.

公开(公告)号：US5622822A

公开(公告)日：1997-04-22

申请号：US305769

申请日：1994-09-13

申请人： Tobias E. Ekeze ,  John W. Backus ,  David J. Sharkey ,  Richard C. Sutton ,  JoAnne H. Kerschner

发明人： Tobias E. Ekeze ,  John W. Backus ,  David J. Sharkey ,  Richard C. Sutton ,  JoAnne H. Kerschner

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/70

CPC分类号： C12N15/1003 ,  C12Q1/6806

摘要： Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.

摘要翻译： 通过使裂解物与聚乙烯亚胺接触以与核酸形成沉淀，可将核酸用于扩增或裂解后的其它处理。 然后通过与强碱接触将核酸从沉淀物中释放，并将释放的核酸与阴离子磷酸酯表面活性剂一起保持在溶液中。 这种制备样品样品的方法简单而且相当迅速。

公开(公告)号：US5308749A

公开(公告)日：1994-05-03

申请号：US646303

申请日：1991-01-25

申请人： Richard C. Sutton ,  Ignazio S. Ponticello ,  Susan J. Danielson ,  Marsha D. B. Oenick

发明人： Richard C. Sutton ,  Ignazio S. Ponticello ,  Susan J. Danielson ,  Marsha D. B. Oenick

IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01N33/545 ,  G01N33/531 ,  G01N33/546

CPC分类号： G01N33/54313 ,  G01N33/54386 ,  Y10S524/90 ,  Y10T428/2991 ,  Y10T428/2998

摘要： Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.

摘要翻译： 生物活性试剂由具有高活性琥珀酰亚胺基团的共聚物颗粒制备。 试剂通过将颗粒表面上的高活性琥珀酰亚胺基团置换而直接或间接地通过酰胺基团将生物活性物质例如抗体共价连接而制备。 这些试剂用于分析元件，检测特异性结合配体（例如免疫物质）和免疫测定的方法以及纯化方法如亲和层析中。

公开(公告)号：US5210289A

公开(公告)日：1993-05-11

申请号：US856279

申请日：1992-03-23

申请人： Ignazio S. Ponticello ,  Richard C. Sutton

发明人： Ignazio S. Ponticello ,  Richard C. Sutton

IPC分类号： C07C69/007 ,  C07C323/12 ,  C07C323/52 ,  C07C323/56 ,  C08F12/14 ,  G01N33/543

CPC分类号： C07C69/007 ,  C07C323/12 ,  C07C323/52 ,  C07C323/56 ,  C08F12/14 ,  G01N33/54313

摘要： Certain ethylenically unsaturated polymerizable monomers having a reactive carboxy group are useful for preparing homo- and copolymers for a variety of uses, including diagnostic assays. The polymers can be supplied as latex particles in aqueous compositions. The monomers are represented by the structure: ##STR1## wherein: R is hydrogen, halo or alkyl of 1 to 3 carbon atoms,M is hydrogen, an alkali metal ion or an ammonium ion, andL is a linking group having from 8 to 50 atoms in its linking chain, and comprises two or more divalent hydrocarbon groups connected or terminated with one or more nitrogen, oxygen or sulfur atoms, or with one or more groups containing such atoms in the linking chain, provided L has at least one arylene which is not directly connected to the terminal ##STR2## group, and further provided that none of the hydrocarbon groups has non-aromatic unsaturation.

摘要翻译： 某些具有活性羧基的烯键式不饱和可聚合单体可用于制备各种用途的均聚物和共聚物，包括诊断测定法。 聚合物可以作为水性组合物中的胶乳颗粒提供。 单体由以下结构表示：其中：R是氢，卤素或1至3个碳原子的烷基，M是氢，碱金属离子或铵离子，L是具有8至 其连接链中包含50个原子，并且包含两个或多个与一个或多个氮，氧或硫原子连接或封端的二价烃基，或者在连接链中与一个或多个含有该原子的基团连接，条件是L具有至少一个亚芳基 其不直接连接到终端基团，并且进一步提供没有一个烃基具有非芳族不饱和键。

公开(公告)号：US5086143A

公开(公告)日：1992-02-04

申请号：US557338

申请日：1990-07-25

申请人： Richard C. Sutton ,  Marsha B. Oenick

发明人： Richard C. Sutton ,  Marsha B. Oenick

IPC分类号： C08F212/02 ,  C08F12/00 ,  C08F220/28 ,  C08F246/00 ,  C08L25/00 ,  G01N33/543 ,  G01N33/545

CPC分类号： G01N33/545 ,  C08F220/28 ,  C08F246/00 ,  G01N33/54313

摘要： Copolymers are prepared from (a) at least about 0.5 mole % of monomers having reactive groups which are, directly or indirectly, capable of reaction with free amine or sulfhydryl groups of biologically active substances, (b) from about 0.1 to about 20 mole % of monomers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88, and (c) up to about 99.4 mole % of oleophilic monomers which provide hydrophobicity to the copolymers. The copolymers can be supplied as latex particles in aqueous compositions.

公开(公告)号：US5053443A

公开(公告)日：1991-10-01

申请号：US347537

申请日：1989-05-04

申请人： Richard C. Sutton

发明人： Richard C. Sutton

IPC分类号： C08J3/215 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58

CPC分类号： C08J3/215 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/56911 ,  G01N33/585

摘要： A polymeric latex composition having a hydrophobe incorporated therein can be prepared using a novel latex "loading" method. This method includes providing a loadable latex, providing a hydrophobe dissolved in a water-miscible organic solvent, heating the loadable latex to about 30.degree. to about 90.degree. C. and gradually adding the hydrophobe solution to the heated loadable latex under conditions to keep the hydrophobe in solution and to "load" it into the latex particles. In preferred embodiments, the latex is surfactant-free and has particles which have outer reactive groups which are capable of reacting with free amine or sulfhydryl groups of a biological compound. Such latices can be used to prepare water-insoluble biological reagents having a biological compound attached to the particles.

摘要翻译： 可以使用新的胶乳“加载”方法制备其中掺入疏水物的聚合物胶乳组合物。 该方法包括提供可加载的胶乳，提供溶解在水可混溶的有机溶剂中的疏水物，将可加载的胶乳加热到约30℃至约90℃，并且在保持该可加载胶乳的条件下逐渐将该疏水物溶液加入到加热的可加载胶乳中 在溶液中疏水，并将其“负载”到胶乳颗粒中。 在优选的实施方案中，胶乳是无表面活性剂的，并且具有能够与生物化合物的游离胺或巯基反应的外部反应性基团的颗粒。 这种胶乳可以用于制备具有附着到颗粒上的生物化合物的水不溶性生物试剂。

公开(公告)号：US4264706A

公开(公告)日：1981-04-28

申请号：US129523

申请日：1980-03-12

申请人： Richard C. Sutton ,  Thomas W. Martin

发明人： Richard C. Sutton ,  Thomas W. Martin

IPC分类号： G03C1/66 ,  C08L33/00 ,  C08L33/24 ,  G02B5/22 ,  G03F7/00 ,  G03F7/038 ,  G03F7/04 ,  H01J29/20 ,  G03C5/00 ,  H01J1/52

CPC分类号： G03F7/04 ,  G03F7/0007 ,  Y10S430/163

摘要： The properties of a dichromated hydrophilic colloid photosensitive composition are improved by the incorporation of certain copolymer latices. More particularly, the copolymer particles in the latex conform to the structure:--A).sub. (B --.phi.-awherein A is a polymerized monomer or monomers having the structure: ##STR1## and B is a polymerized monomer or monomers having the structure: ##STR2## wherein each R.sup.1 is independently hydrogen or methyl;R.sup.2 is phenyl, substituted phenyl or a straight- or branched-chain alkoxycarbonyl group of about 2-8 carbon atoms;R.sup.3 is --NR.sup.4 R.sup.5 or --OR.sup.6 wherein R.sup.4 and R.sup.5 are independently selected from hydrogen, alkyl of about 1-8 carbon atoms including substituted alkyl, and alkyl containing hetero atoms in the alkyl chain, and R.sup.6 is hydroxyalkyl of about 1-8 carbon atoms; anda represents a weight percent of about 75-95.

摘要翻译： 通过掺入某些共聚物胶乳改善了重铬酸酯亲水胶体感光组合物的性能。 更具体地说，乳液中的共聚物颗粒符合以下结构：-A）（B-phi -a其中A是聚合单体或具有以下结构的单体：＆lt; IMAGE＆gt;和B是聚合单体或具有以下结构的单体： 其中每个R 1独立地是氢或甲基; R 2是苯基，取代的苯基或约2-8个碳原子的直链或支链烷氧基羰基; R 3是-NR 4 R 5或-OR 6，其中R 4和R 5独立地选自 氢原子，含有取代烷基的约1-8个碳原子的烷基和烷基链中含有杂原子的烷基，R 6是约1-8个碳原子的羟基烷基; a代表约75-95的重量百分比。