发明授权
- 专利标题： Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
- 专利标题（中）： 使用聚乙烯亚胺和阴离子磷酸酯表面活性剂捕获和选择性释放核酸的方法及其扩增
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申请号： US305769申请日： 1994-09-13
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公开(公告)号： US5622822A公开(公告)日： 1997-04-22
- 发明人: Tobias E. Ekeze , John W. Backus , David J. Sharkey , Richard C. Sutton , JoAnne H. Kerschner
- 申请人： Tobias E. Ekeze , John W. Backus , David J. Sharkey , Richard C. Sutton , JoAnne H. Kerschner
- 申请人地址： NY Rochester
- 专利权人： Johnson & Johnson Clinical Diagnostics, Inc.
- 当前专利权人： Johnson & Johnson Clinical Diagnostics, Inc.
- 当前专利权人地址： NY Rochester
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C07H21/04 ; C12N15/10 ; C12Q1/68 ; C12P19/34 ; C12Q1/70
摘要：
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.
公开/授权文献
- US4375260A Cuboid cigarettes or cigars pack or the like 公开/授权日：1983-03-01
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