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    Method for detection of a nucleic acid using particulate reagent having polyoxyalkylene side chains
    2.
    发明授权
    Method for detection of a nucleic acid using particulate reagent having polyoxyalkylene side chains 失效
    使用具有聚氧化烯侧链的颗粒试剂检测核酸的方法

    公开(公告)号：US5330891A

    公开(公告)日：1994-07-19

    申请号：US968936

    申请日：1992-10-30

    申请人： Richard C. Sutton ,  Marsha B. Oenick

    发明人： Richard C. Sutton ,  Marsha B. Oenick

    IPC分类号： C07K17/02 ,  C12Q1/68 ,  G01N31/22 ,  G01N33/48 ,  G01N33/543 ,  G01N33/545 ,  C12Q1/70 ,  C08F12/16 ,  C08L33/00 ,  G01N21/00

    CPC分类号： G01N33/54313 ,  C07K17/02 ,  C12Q1/6834 ,  Y10S435/962 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/974 ,  Y10S435/975

    摘要： Biologically active reagents are prepared from particles of copolymers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through reactive groups on the particle surface. These reagents are used to advantage in analytical elements and methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents. Adsorption of undesirable proteins on the particles of the reagents was considerably reduced because of the specific composition of the particles.

    摘要翻译： 生物活性试剂由具有聚氧化烯侧链的共聚物颗粒制备，每个侧链具有至少约88的分子量。试剂通过将生物活性物质，例如抗体直接或间接地附着到颗粒上而制备， 间接地通过颗粒表面上的反应性基团。 这些试剂用于检测特异性结合配体（如免疫物质）和免疫测定的分析元件和方法，以及作为亲和层析试剂的纯化方法。 由于颗粒的特定组成，吸附不需要的蛋白质在试剂颗粒上显着降低。

    Avidin-and biotin immobilized reagents and methods of use
    4.
    发明授权
    Avidin-and biotin immobilized reagents and methods of use 失效
    抗生物素蛋白和生物素固定化试剂及使用方法

    公开(公告)号：US5374516A

    公开(公告)日：1994-12-20

    申请号：US315086

    申请日：1989-02-24

    申请人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

    发明人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

    IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/546

    CPC分类号： G01N33/54306 ,  G01N33/54313 ,  G01N33/545 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

    摘要翻译： 试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。 聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。 亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。 所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。 固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。

    Carboxy containing monomers
    10.
    发明授权
    Carboxy containing monomers 失效
    含羧基的单体

    公开(公告)号：US5210289A

    公开(公告)日：1993-05-11

    申请号：US856279

    申请日：1992-03-23

    申请人： Ignazio S. Ponticello ,  Richard C. Sutton

    发明人： Ignazio S. Ponticello ,  Richard C. Sutton

    IPC分类号： C07C69/007 ,  C07C323/12 ,  C07C323/52 ,  C07C323/56 ,  C08F12/14 ,  G01N33/543

    CPC分类号： C07C69/007 ,  C07C323/12 ,  C07C323/52 ,  C07C323/56 ,  C08F12/14 ,  G01N33/54313

    摘要： Certain ethylenically unsaturated polymerizable monomers having a reactive carboxy group are useful for preparing homo- and copolymers for a variety of uses, including diagnostic assays. The polymers can be supplied as latex particles in aqueous compositions. The monomers are represented by the structure: ##STR1## wherein: R is hydrogen, halo or alkyl of 1 to 3 carbon atoms,M is hydrogen, an alkali metal ion or an ammonium ion, andL is a linking group having from 8 to 50 atoms in its linking chain, and comprises two or more divalent hydrocarbon groups connected or terminated with one or more nitrogen, oxygen or sulfur atoms, or with one or more groups containing such atoms in the linking chain, provided L has at least one arylene which is not directly connected to the terminal ##STR2## group, and further provided that none of the hydrocarbon groups has non-aromatic unsaturation.

    摘要翻译： 某些具有活性羧基的烯键式不饱和可聚合单体可用于制备各种用途的均聚物和共聚物，包括诊断测定法。 聚合物可以作为水性组合物中的胶乳颗粒提供。 单体由以下结构表示：其中：R是氢，卤素或1至3个碳原子的烷基，M是氢，碱金属离子或铵离子，L是具有8至 其连接链中包含50个原子，并且包含两个或多个与一个或多个氮，氧或硫原子连接或封端的二价烃基，或者在连接链中与一个或多个含有该原子的基团连接，条件是L具有至少一个亚芳基 其不直接连接到终端基团，并且进一步提供没有一个烃基具有非芳族不饱和键。