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公开(公告)号：US5527709A
公开(公告)日：1996-06-18
申请号：US410548
申请日：1995-03-23
IPC分类号： C12N9/08 , G01N33/78 , G01N33/545
CPC分类号： C12N9/0065 , G01N33/78 , Y10S436/817
摘要： The invention is directed to an immunoassay for a thyronine derivative comprising:A. contacting a liquid sample, containing a thyronine derivative, with a labeled thyronine hapten analogue of the derivative in the presence of antibodies for the thyronine derivative under conditions that promote the formation of antibody-thyronine immunocomplexes; andB. determining the bound or unbound quantity of the thyronine derivative in the liquid; characterized in that the labeled thyronine derivative comprises:(i) a label, of the type used in immunoassays, having an amine or sulfhydyl group;(ii) a thyronine nucleus and(iii) a linking chain linking the label to the thyronine nucleus.
摘要翻译： 本发明涉及一种对于神钢衍生物的免疫测定，其包括：A.在促进形成甲状腺激素的条件下，在含有甲状腺导向抗体的抗体存在下，将含有甲状腺衍生物的液体样品与衍生物的标记的甲状腺恶性半抗原类似物接触 抗体 - 甲状腺免疫复合物; 和B.确定液体中的甲状腺衍生物的结合或未结合量; 其特征在于，所述标记的甲状腺衍生物包含：（i）在免疫测定中使用的具有胺或巯基的类型的标签; （ii）神经细胞核和（iii）连接标签与陀螺质核的连接链。
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公开(公告)号：US5374516A
公开(公告)日：1994-12-20
申请号：US315086
申请日：1989-02-24
申请人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
发明人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
IPC分类号： G01N33/543 , G01N33/545 , G01N33/546
CPC分类号： G01N33/54306 , G01N33/54313 , G01N33/545 , Y10T428/2991 , Y10T428/2998
摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
摘要翻译： 试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。 聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。 亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。 所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。 固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。
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3.发明授权Specific binding analytical and separation methods using carboxy containing polymers 失效使用含羧基聚合物的特异性结合分析和分离方法
公开(公告)号：US5262297A
公开(公告)日：1993-11-16
申请号：US876672
申请日：1992-04-30
申请人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred. T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
发明人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred. T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/543 , G01N33/545 , G01N83/545 , G01N33/546 , G01N33/547
CPC分类号： G01N33/54313 , C12Q1/6876 , C12Q1/701 , C12Q1/703 , G01N33/545 , Y10S436/804 , Y10S436/814 , Y10S436/817 , Y10S436/818 , Y10S436/824
摘要： Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.
摘要翻译： 特异性结合方法用于诊断测定和纯化分离，由此特异性结合捕获试剂由具有高反应性羧基的共聚物制备。 这些基团从聚合物表面延伸，在链中具有8至50个原子的连接基团和两个或更多个亚烷基，亚芳基，亚烷基亚芳基或亚芳基亚烷基。 将这些反应性基团连接到生物活性物质如蛋白质或寡核苷酸上，然后参与诊断测定或纯化分离方法。
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4.发明授权
公开(公告)号：US5162219A
公开(公告)日：1992-11-10
申请号：US540428
申请日：1990-06-18
IPC分类号： C07K1/13 , C07K14/00 , C12N9/08 , G01N33/535 , G01N33/543
CPC分类号： G01N33/535 , G01N33/54386
摘要： Amine-enriched proteins, having an increased isoelectric point are provided by reacting a naturally occurring protein with an amide bond forming agent in the presence of a polyamine or a salt thereof. Such amine-enriched proteins such as enzymes are useful as labels in immunoassays.
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5.发明授权Fluorescent labels and labeled species and their use in analytical elements and determinations 失效荧光标签和标记物，以及它们在分析元素和测定中的应用
公开(公告)号：US4719182A
公开(公告)日：1988-01-12
申请号：US713206
申请日：1985-03-18
IPC分类号： C08F8/00 , C08G81/00 , C08G81/02 , G01N33/52 , G01N33/533 , G01N33/543 , G01N33/545 , G01N33/58 , G01N33/546 , G01N33/547
CPC分类号： G01N33/582 , G01N33/525 , G01N33/533 , G01N33/54386 , G01N33/585 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T428/2991
摘要： Fluorescent labels comprise a polysaccharide bound to a polymeric particle which contains a fluorescent rare earth chelate. These labels can be attached to any of a variety of physiologically reactive species to provide labeled species which have improved stability in aqueous solutions. The labeled species are particularly useful in specific binding assays to determine an immunologically reactive ligand, e.g. a hapten, in either solution or dry analytical techniques.
摘要翻译： 荧光标记物包含与含有荧光稀土螯合物的聚合物颗粒结合的多糖。 这些标签可以连接到各种生理反应性物质中的任一种，以提供在水溶液中具有改善的稳定性的标记物质。 标记的物种在特异性结合测定中特别有用，以确定免疫反应性配体，例如， 半抗原，溶液或干法分析技术。
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6.发明授权Heterogeneous immunoassay utilizing horizontal separation in an analytical element 失效在分析元件中利用水平分离的非均相免疫测定
公开(公告)号：US4670381A
公开(公告)日：1987-06-02
申请号：US757111
申请日：1985-07-19
申请人： Paul H. Frickey , Karl J. Sanford , Glen M. Dappen , Allen L. Thunberg , Michael W. Sundberg , Susan J. Danielson
发明人： Paul H. Frickey , Karl J. Sanford , Glen M. Dappen , Allen L. Thunberg , Michael W. Sundberg , Susan J. Danielson
IPC分类号： G01N33/543 , G01N33/52 , G01N33/538 , G01N33/50 , G01N33/545 , G01N33/558
CPC分类号： G01N33/538 , G01N33/526 , Y10S435/97 , Y10S436/81 , Y10S436/816
摘要： A heterogeneous, competitive binding immunoassay is conducted with a dry analytical element. The immunoassay is useful for determining a ligand (e.g. a therapeutic drug) and includes contacting a finite area of the element spreading layer with a sample of a liquid in the presence of a labeled ligand analog and an immobilized receptor. An immobilized ligand-receptor complex is formed within the finite area. Simultaneously, the uncomplexed ligand migrates horizontally away from the immobilized complex which remains in the center of the finite area. At least five seconds after the completion of the contacting, the amount of immobilized complex is measured in the center of the finite area.
摘要翻译： 用干燥的分析元件进行异质竞争性结合免疫测定。 免疫测定可用于测定配体（例如治疗药物），并且包括在标记的配体类似物和固定化受体的存在下使元件铺展层的有限区域与液体样品接触。 在有限区域内形成固定化的配体 - 受体复合体。 同时，未络合的配体水平地迁移离开固定复合物，其保留在有限区域的中心。 接触完成后至少5秒钟，在有限区域的中心测量固定化复合物的量。
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7.发明授权Method of preparing biologically active reagents from succinimide-containing polymers, analytical element and methods of use 失效从含琥珀酰亚胺的聚合物制备生物活性试剂的方法，分析元件和使用方法
公开(公告)号：US5308749A
公开(公告)日：1994-05-03
申请号：US646303
申请日：1991-01-25
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/545 , G01N33/531 , G01N33/546
CPC分类号： G01N33/54313 , G01N33/54386 , Y10S524/90 , Y10T428/2991 , Y10T428/2998
摘要： Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.
摘要翻译： 生物活性试剂由具有高活性琥珀酰亚胺基团的共聚物颗粒制备。 试剂通过将颗粒表面上的高活性琥珀酰亚胺基团置换而直接或间接地通过酰胺基团将生物活性物质例如抗体共价连接而制备。 这些试剂用于分析元件，检测特异性结合配体（例如免疫物质）和免疫测定的方法以及纯化方法如亲和层析中。
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公开(公告)号：US5284948A
公开(公告)日：1994-02-08
申请号：US851435
申请日：1992-03-16
IPC分类号： G01N33/535 , C07D403/12 , G01N33/532 , G01N33/94 , C07D403/00 , C07D233/72 , C07D239/02
CPC分类号： C07D403/12 , G01N33/532 , G01N33/94 , G01N33/948
摘要： The invention is directed to new drug hapten analogues comprising:(A) an active ester group;(B) a drug hapten nucleus selected from a hydantoin nucleus or a barbiturate nucleus and(C) a linking chain linking the 3-position of the drug hapten nucleus to the active ester group.
摘要翻译： 本发明涉及新的药物半抗原类似物，其包括：（A）活性酯基; （B）选自乙内酰脲核或巴比妥酸核的药物半抗原核和（C）连接药物半抗原核的3位与活性酯基的连接链。
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9.发明授权Biologically active reagent prepared from aldehyde-containing polymer, test kit, analytical element and methods of use 失效由含醛聚合物，试剂盒，分析元素和使用方法制备的生物活性试剂
公开(公告)号：US5401633A
公开(公告)日：1995-03-28
申请号：US955167
申请日：1992-10-01
IPC分类号： G01N33/50 , C12Q1/68 , G01N33/543 , G01N33/545 , G01N33/68 , G01N33/546
CPC分类号： C12Q1/6834 , G01N33/54313 , G01N33/54386 , G01N33/545
摘要： A biologically active reagent is prepared by reacting a biologically active substance covalently to a polymeric particle having pendant aldehyde groups. Such groups are provided by a polymerizable monomer represented by the structure: ##STR1## wherein Ar is arylene, R and R.sup.1 are independently hydrogen, halo or lower alkyl, R.sup.2 and R.sup.3 are independently alkylene of 1 to 4 carbon atoms in the chain, R.sup.4 is arylene, m is 0 or 1, and n is an integer of 1 to 4. These reagents can be used in a variety of specific binding and affinity purification methods, and in both solution assays and dry analytical elements.
摘要翻译： 通过将生物活性物质共价反应到具有侧基醛基的聚合物颗粒来制备生物活性试剂。 这样的基团由以下结构表示的可聚合单体提供：其中Ar是亚芳基，R和R 1独立地是氢，卤素或低级烷基，R 2和R 3独立地是链中具有1至4个碳原子的亚烷基，R 4 是亚芳基，m是0或1，n是1至4的整数。这些试剂可以用于各种特异性结合和亲和纯化方法，以及在溶液测定和干燥分析元件中。
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10.发明授权Attachment of compounds to polymeric particles using carbamoylonium compounds and a kit containing same 失效使用氨基甲酸铵化合物将化合物与聚合物颗粒附着，以及含有它们的试剂盒
公开(公告)号：US5397695A
公开(公告)日：1995-03-14
申请号：US373304
申请日：1989-06-29
IPC分类号： B01D61/18 , B01D67/00 , B01D69/12 , G01N33/53 , G01N33/543 , C12Q1/70 , C12N11/08 , C07K3/00
CPC分类号： B01D67/0088 , B01D61/18 , B01D69/12 , G01N33/5302 , G01N33/54366 , Y10S435/81 , Y10S435/975
摘要： Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, such as enzymes, polypeptides and proteins. This attachment is carried out using carbamoylonium compounds which react with the carboxyl groups to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a carbamoylonium compound.
摘要翻译： 用于诊断测试，亲和层析，酶促反应和免疫测定的有用材料通过将包含反应性氨基或巯基的反应性化合物共价连接到在外表面上具有侧基羧基的聚合物颗粒来制备。 这种反应性化合物包括生物反应性物质，例如酶，多肽和蛋白质。 该连接使用与羧基反应形成中间反应基团的氨基甲酰胺化合物进行，然后与氨基或巯基反应形成颗粒和反应性化合物之间的共价连接。 试剂盒包括在外表面上具有羧基的聚合物颗粒和氨基甲酰胺化合物。
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