公开(公告)号：US5407568A

公开(公告)日：1995-04-18

申请号：US118704

申请日：1993-09-09

Applicant: Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

Inventor： Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

IPC: G01N30/02 ,  B01D15/08 ,  B01J39/26 ,  G01N30/50 ,  G01N30/96

CPC classification number: G01N30/96 ,  B01D15/20 ,  B01D15/363 ,  B01J39/26

Abstract: Deterioration of performance of a separation column for liquid chromatograph due to changes in the functional groups during the preservation or application is prevented by filling a solution containing a component in the molecule with the same structure as that of the chemical function group of a packing material for the separation column as a filling liquid together with the packing material in the separation column, and also by washing the separation column with the same filling liquid as a washing solution after the analysis and retaining the washing solution therein, whereby the preservation stability of the separation column is improved and the separation column can be preserved at room temperature.

Abstract translation: 通过将包含分子中的成分的溶液与包装材料的化学官能团相同的结构填充来防止由于保存或施加期间官能团的变化而导致的液相色谱分离柱的性能劣化 分离塔作为填充液与分离塔中的包装材料一起，并且在分析后保持与洗涤液相同的填充液，同时通过洗涤分离塔，从而保持分离的保存稳定性 柱被改善，分离柱可以在室温下保存。

公开(公告)号：US5358639A

公开(公告)日：1994-10-25

申请号：US38396

申请日：1993-03-29

Applicant: Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

Inventor： Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

IPC: G01N30/26 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N30/50 ,  G01N30/52 ,  G01N30/88 ,  G01N33/72 ,  B01D15/08

CPC classification number: G01N30/52 ,  G01N2030/525 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N33/72

Abstract: In the analysis of hemoglobins in blood samples by chromatography, an increase in the pressure in a flow passage line is suppressed, a separation column is made durable against prolonged use by supplying phosphate-based buffer solutions as eluting solutions to a separation column to a separation column and a solution containing not more than 100 mM of S-(carboxyalkyl)-L-cysteine and phosphate-based buffer agent thereto in the course of a series of analyzing steps.

Abstract translation: 在通过色谱法对血液样品中的血红蛋白进行分析时，抑制流路线中的压力增加，通过将磷酸盐类缓冲溶液作为分离柱的洗脱溶液供给至分离液，使分离塔长期使用耐久性 柱和在一系列分析步骤中含有不超过100mM的S-（羧基烷基）-L-半胱氨酸和磷酸盐缓冲剂的溶液。

公开(公告)号：US5468379A

公开(公告)日：1995-11-21

申请号：US257306

申请日：1994-06-09

Applicant: Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

Inventor： Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

IPC: G01N30/26 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N30/50 ,  G01N30/52 ,  G01N30/88 ,  G01N33/72 ,  B01D15/08

CPC classification number: G01N30/52 ,  G01N2030/525 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N33/72

Abstract: In the analysis of hemoglobins in blood samples by chromatography, an increase in the pressure in a flow passage line is suppressed, a separation column is made durable against prolonged use by supplying phosphate-based buffer solutions as eluting solutions to a separation column and a solution containing not more than 100 mM of S-(carboxyalkyl)-L-cysteine and phosphate-based buffer agent thereto in the course of a series of analyzing steps.

Abstract translation: 在通过色谱法对血液样品中的血红蛋白进行分析时，抑制流路线中的压力增加，通过向分离塔和溶液提供磷酸盐缓冲溶液作为洗脱溶液，使分离柱长期使用耐久性 在一系列分析步骤中含有不超过100mM的S-（羧基烷基）-L-半胱氨酸和磷酸盐缓冲剂。

公开(公告)号：US09447447B2

公开(公告)日：2016-09-20

申请号：US13876992

申请日：2011-09-30

Applicant: Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

Inventor： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

IPC: C12M1/34 ,  C12Q1/02 ,  C12M1/42 ,  C12M1/36 ,  G01N33/50

CPC classification number: C12Q1/025 ,  C12M35/02 ,  C12M41/46 ,  C12M41/48 ,  G01N33/48728 ,  G01N33/5061 ,  G01N33/5088 ,  G01N2800/32

Abstract: In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

Abstract translation: 在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。 将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。 通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

公开(公告)号：US08361412B2

公开(公告)日：2013-01-29

申请号：US11317004

申请日：2005-12-27

Applicant: Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

Inventor： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

IPC: B01L3/00

CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

Abstract translation: 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。

公开(公告)号：US08247222B2

公开(公告)日：2012-08-21

申请号：US13036600

申请日：2011-02-28

Applicant: Akihiro Hattori ,  Kenji Yasuda

Inventor： Akihiro Hattori ,  Kenji Yasuda

IPC: C12M1/00 ,  C12M1/34

CPC classification number: C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

Abstract: This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

Abstract translation: 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，吸收 在可见光和红外区域具有吸收性的层，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及具有特定波长的单色光的光源，其中光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。

公开(公告)号：US07678777B2

公开(公告)日：2010-03-16

申请号：US12190121

申请日：2008-08-12

Applicant: Kenji Yasuda ,  Tomohiko Ogawa ,  Masakatsu Hasegawa

Inventor： Kenji Yasuda ,  Tomohiko Ogawa ,  Masakatsu Hasegawa

IPC: A61K31/721 ,  A61K35/74 ,  C08B37/02

CPC classification number: C12N1/38 ,  A23K20/163 ,  A23L29/273 ,  A23L33/135 ,  A23V2002/00 ,  C12N1/20 ,  A23V2200/3204 ,  A23V2250/5042 ,  A23V2200/3202

Abstract: A composition for promoting bacterial proliferation and selectively proliferating Lactobacillus casei subsp. casei is disclosed, which includes a dextran. A variety of biological activities originating from L. casei subsp. casei can be sustained in a living body by selectively growing-proliferating and colonizing L. casei subsp. casei in the intestine of a human being, animal, or the like or by selectively growing-proliferating L. casei subsp. casei in the intestine, without supplying L. casei subsp. casei at all times.

Abstract translation: 用于促进细菌增殖和选择性增殖的干酪乳杆菌亚种的组合物。 公开了干酪素，其包括葡聚糖。 来自干酪乳杆菌亚种的各种生物活性 干酪素可以通过选择性生长增殖和殖民化的干酪乳杆菌亚种，维持在活体内。 在人，动物等的肠中或通过选择性增殖增殖的干酪乳杆菌 肠道中的酪蛋白，不提供干酪乳杆菌。 干酪素在任何时候。

公开(公告)号：US20080003678A1

公开(公告)日：2008-01-03

申请号：US11819681

申请日：2007-06-28

Applicant: Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

Inventor： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

IPC: C12N5/06 ,  C12M1/00

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  C12M45/00 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/12 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1081 ,  G01N2015/149

Abstract: An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.

Abstract translation: 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。

公开(公告)号：US07217573B1

公开(公告)日：2007-05-15

申请号：US09678652

申请日：2000-10-04

Applicant: Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

Inventor： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

IPC: G01N33/00 ,  G01N33/53 ,  C12Q1/68 ,  C12M3/00 ,  C07H21/04 ,  H01J3/14

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  Y10S436/80 ,  Y10T436/143333

Abstract: A method of inspecting a DNA chip and an apparatus therefor that allow a picture to be reconstructed in the following steps: A plurality of irradiation spots are formed on a DNA probe array mounted on a stage. Then, the stage is displaced in X, Y directions so as to execute a scanning, thereby irradiating substantially all the entire surface of the DNA probe array. Next, a plurality of emitted fluorescent lights, which are generated from the plurality of irradiation spot portions on the DNA probe array, are converged and are then detected simultaneously by multi detectors. Finally, a data processing apparatus processes the detected signals, thereby reconstructing the picture.

Abstract translation: 一种用于检查DNA芯片的方法及其装置，其允许以下列步骤重建图像：在安装在平台上的DNA探针阵列上形成多个照射点。 然后，在X，Y方向上移位台，以进行扫描，从而基本上照射DNA探针阵列的整个表面。 接下来，从DNA探针阵列上的多个照射点部分产生的多个发射荧光灯被会聚，然后由多个检测器同时检测。 最后，数据处理装置处理检测到的信号，从而重建图像。

公开(公告)号：US20060194307A1

公开(公告)日：2006-08-31

申请号：US11317004

申请日：2005-12-27

Applicant: Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

Inventor： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

IPC: C12M1/34 ,  B07C5/00

CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

Abstract translation: 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。