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Patent Agency Ranking
公开(公告)号：US20150202618A1
公开(公告)日：2015-07-23
申请号：US14421239
申请日：2013-07-08
Applicant: Hitachi High-Technologies Corporation
Inventor： Takanobu Haga , Yoshitaka Kodama , Tomohiro Shoji , Takamichi Muramatsu , Shuhei Yamamoto
CPC classification number: B01L3/50 , B01L3/5027 , B01L99/00 , B01L2300/0636 , C12Q1/6869 , C12Q2535/122 , G01N21/6428 , C12Q2563/107 , C12Q2565/629
Abstract: A nucleic acid analysis device which can determine a DNA sequence has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized. At least a part of the flowcell is made of a transparent material. An irradiation unit irradiates a part in which the DNA fragment clusters are immobilized. The device has a lens for collecting fluorescence, and a light-detection element. A solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution are sent sequentially to where the DNA fragment clusters are immobilized.
Abstract translation: 可以确定DNA序列的核酸分析装置具有其中固定有两个或更多个具有相同核苷酸序列的DNA片段的两个或更多个DNA片段的流式细胞池。 流通池的至少一部分由透明材料制成。 照射单元照射固定有DNA片段簇的部分。 该装置具有用于收集荧光的透镜和光检测元件。 在四个碱中仅含有具有荧光改性磷酸酯末端的dATP的溶液，仅含有四碱基中具有荧光改性磷酸酯末端的dCTP的溶液，仅含有四碱基中具有荧光改性磷酸酯末端的dGTP的溶液，含有 在四个碱基中只有具有荧光改性的磷酸酯末端的dTTP和缓冲溶液依次送到固定有DNA片段簇的位置。
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公开(公告)号：US09971139B2
公开(公告)日：2018-05-15
申请号：US15305226
申请日：2015-03-12
Applicant: Hitachi High-Technologies Corporation
Inventor： Michiru Fujioka , Shuhei Yamamoto , Tsuyoshi Sonehara , Hiromi Kusaka , Akimasa Osaka
CPC classification number: G02B21/33 , G02B21/082 , G02B21/12 , G02B21/26 , G02B21/34
Abstract: In the microscope observation container according to the present invention, an observed sample is accommodated by an objective lens barrel provided with a housing extending along the radiation direction of excitation light and an objective lens fixed to an inside surface of the housing. The microscope observation container is provided with a structure for collecting a liquid immersion medium added by dispensation, the structure having a portion contacted by the objective lens barrel during observation. During observation the aforementioned portion is contacted by the objective lens barrel, and the liquid immersion medium is thereby sealed by the objective lens barrel and the structure. The aforementioned portion also has an elastic force, and is deformed so as to conform to the housing of the objective lens by the contact.
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公开(公告)号：US09804029B2
公开(公告)日：2017-10-31
申请号：US15120792
申请日：2015-01-16
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Tsuyoshi Sonehara , Hiromi Kusaka , Akira Fujii , Shuhei Yamamoto , Takeshi Ooura , Michiru Fujioka
IPC: G01J3/30 , G01J3/42 , G01J3/24 , G01J3/44 , G02B7/28 , G02B7/32 , G01N21/65 , G02B21/36 , G01N21/64 , G01J3/02 , G01J3/10 , G02B21/00 , G02B21/16 , G02B21/24 , G02B27/14
CPC classification number: G01J3/44 , G01J3/0208 , G01J3/021 , G01J3/0229 , G01J3/0248 , G01J3/10 , G01J3/4406 , G01N21/6458 , G01N21/65 , G02B7/28 , G02B7/32 , G02B21/0004 , G02B21/16 , G02B21/244 , G02B21/36 , G02B21/365 , G02B27/141
Abstract: With a microspectroscopy device provided with an objective lens with a high numerical aperture, a defocus arises from thermal drift, etc., necessitating auto-focusing. Conventional auto-focus based on through-focus image acquisition takes time, and thus, it cannot be applied to continuous measurement over a long time wherein high-speed sampling is carried out. The present invention addresses this problem by having a defocus-sensing beam that has either defocus or astigmatism fall incident on the objective lens. Since how the image of the spot of the beam for defocus sensing blurs differs depending on the orientation of the defocus, real-time detection of the amount and orientation of defocus becomes possible, and high-speed realtime auto-focus becomes possible.
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公开(公告)号：US09770713B2
公开(公告)日：2017-09-26
申请号：US14421239
申请日：2013-07-08
Applicant: Hitachi High-Technologies Corporation
Inventor： Takanobu Haga , Yoshitaka Kodama , Tomohiro Shoji , Takamichi Muramatsu , Shuhei Yamamoto
CPC classification number: B01L3/50 , B01L3/5027 , B01L99/00 , B01L2300/0636 , C12Q1/6869 , C12Q2535/122 , G01N21/6428 , C12Q2563/107 , C12Q2565/629
Abstract: A nucleic acid analysis device which can determine a DNA sequence has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized. At least a part of the flowcell is made of a transparent material. An irradiation unit irradiates a part in which the DNA fragment clusters are immobilized. The device has a lens for collecting fluorescence, and a light-detection element. A solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution are sent sequentially to where the DNA fragment clusters are immobilized.
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公开(公告)号：US09523114B2
公开(公告)日：2016-12-20
申请号：US14508338
申请日：2014-10-07
Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
Inventor： Minoru Sano , Masato Ishizawa , Shuhei Yamamoto
IPC: C12M1/34 , C12M3/00 , C12Q1/68 , G01N21/64 , G01N21/66 , G01N35/02 , G01N21/03 , G01N35/00 , B01L7/00
CPC classification number: C12Q1/68 , B01L7/52 , G01N21/0332 , G01N21/645 , G01N21/6452 , G01N35/025 , G01N2035/00366
Abstract: An object of the present invention relates to providing a nucleic acid analyzer capable of testing a plurality of test items in parallel, and of obtaining high efficiency of specimen processing even if the test item or a measuring object is changed. The present invention relates to an analyzer including a carousel rotatable about a rotation axis, a plurality of reaction containers held along a circumferential edge of the carousel, and at least one detector having a light source for irradiating the reaction container with excitation light and a detection element for detecting fluorescence from a reaction liquid in the reaction container. The detector is removable. By attaching a desired detector, it is possible to perform fluorescence measurement in response to the test item. According to the present invention, it is possible to test a plurality of test items in parallel, and even if the test item or the measuring object is changed, the high efficiency of specimen processing can be obtained.
Abstract translation: 本发明的目的是提供一种能够并行地测试多个测试项目的核酸分析仪，并且即使测试项目或测量对象改变也能获得高效率的样本处理。 本发明涉及一种分析仪，其包括可围绕旋转轴线旋转的圆盘传送带，沿圆盘传送带的圆周边缘保持的多个反应容器，以及至少一个检测器，具有用于向反应容器照射激发光和检测的光源 用于检测来自反应容器中的反应液体的荧光的元件。 检测器是可拆卸的。 通过附加所需的检测器，可以响应于测试项目执行荧光测量。 根据本发明，可以并行地进行多个测试项目的测试，即使测试项目或测量对象发生变化，也能够获得高效的样本处理。
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