公开(公告)号：US10031083B2

公开(公告)日：2018-07-24

申请号：US15518341

申请日：2014-10-16

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Michiru Fujioka ,  Tsuyoshi Sonehara ,  Naoshi Itabashi

IPC: G01J3/44 ,  G01N21/65 ,  G01N33/487 ,  G01Q60/22 ,  G11B23/03 ,  G11B7/08 ,  G11B7/1387 ,  G11B7/085 ,  G11B7/22

Abstract: The purpose of the present invention is to control, with a simple structure and high accuracy, irradiation of excitation light to a multi-nanopore substrate without interrupting a measurement. Irradiation of excitation light is performed concurrently to at least one nanopore and at least one reference object on a substrate mounted in an observation container 103. A position irradiated with the excitation light in a measurement sample is calculated on the basis of a signal generated from the reference object detected by a detector 109, and the measurement and a fixed position control is performed concurrently by performing measurement of the measurement object while a drive control part 115 controlling the position of the irradiation of the excitation light to the measurement sample on the basis of the calculation result, whereby an analysis of the measurement sample can be performed in a short time.

公开(公告)号：US09971139B2

公开(公告)日：2018-05-15

申请号：US15305226

申请日：2015-03-12

Applicant: Hitachi High-Technologies Corporation

Inventor： Michiru Fujioka ,  Shuhei Yamamoto ,  Tsuyoshi Sonehara ,  Hiromi Kusaka ,  Akimasa Osaka

IPC: G02B21/33 ,  G02B21/08 ,  G02B21/12 ,  G02B21/26 ,  G02B21/34

CPC classification number: G02B21/33 ,  G02B21/082 ,  G02B21/12 ,  G02B21/26 ,  G02B21/34

Abstract: In the microscope observation container according to the present invention, an observed sample is accommodated by an objective lens barrel provided with a housing extending along the radiation direction of excitation light and an objective lens fixed to an inside surface of the housing. The microscope observation container is provided with a structure for collecting a liquid immersion medium added by dispensation, the structure having a portion contacted by the objective lens barrel during observation. During observation the aforementioned portion is contacted by the objective lens barrel, and the liquid immersion medium is thereby sealed by the objective lens barrel and the structure. The aforementioned portion also has an elastic force, and is deformed so as to conform to the housing of the objective lens by the contact.

公开(公告)号：US09804029B2

公开(公告)日：2017-10-31

申请号：US15120792

申请日：2015-01-16

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Tsuyoshi Sonehara ,  Hiromi Kusaka ,  Akira Fujii ,  Shuhei Yamamoto ,  Takeshi Ooura ,  Michiru Fujioka

IPC: G01J3/30 ,  G01J3/42 ,  G01J3/24 ,  G01J3/44 ,  G02B7/28 ,  G02B7/32 ,  G01N21/65 ,  G02B21/36 ,  G01N21/64 ,  G01J3/02 ,  G01J3/10 ,  G02B21/00 ,  G02B21/16 ,  G02B21/24 ,  G02B27/14

CPC classification number: G01J3/44 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0248 ,  G01J3/10 ,  G01J3/4406 ,  G01N21/6458 ,  G01N21/65 ,  G02B7/28 ,  G02B7/32 ,  G02B21/0004 ,  G02B21/16 ,  G02B21/244 ,  G02B21/36 ,  G02B21/365 ,  G02B27/141

Abstract: With a microspectroscopy device provided with an objective lens with a high numerical aperture, a defocus arises from thermal drift, etc., necessitating auto-focusing. Conventional auto-focus based on through-focus image acquisition takes time, and thus, it cannot be applied to continuous measurement over a long time wherein high-speed sampling is carried out. The present invention addresses this problem by having a defocus-sensing beam that has either defocus or astigmatism fall incident on the objective lens. Since how the image of the spot of the beam for defocus sensing blurs differs depending on the orientation of the defocus, real-time detection of the amount and orientation of defocus becomes possible, and high-speed realtime auto-focus becomes possible.