-
1.发明授权公开(公告)号：US08158410B2
公开(公告)日：2012-04-17
申请号：US11038920
申请日：2005-01-18
申请人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
发明人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
CPC分类号： G01N1/40 , G01N1/405 , Y10T436/25375
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。
-
公开(公告)号：US5585481A
公开(公告)日：1996-12-17
申请号：US182666
申请日：1994-01-14
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这种试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者各自具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
-
公开(公告)号：US5449769A
公开(公告)日：1995-09-12
申请号：US319570
申请日：1989-03-06
申请人： Ram S. Bhatt
发明人： Ram S. Bhatt
IPC分类号： C07H1/00 , C07B47/00 , C07F9/165 , C07F9/40 , C07F9/655 , C07F9/6561 , C07H19/10 , C07H19/207 , C07H21/00 , C07H15/12 , C07H17/00
CPC分类号： C07H21/00 , C07F9/165 , C07F9/65515 , C07F9/65616
摘要： A composition suitable for sulfurizing an organophosphite. The reagent comprises a solution of sulfur and a tertiary amine, in a suitable solvent such as carbon disulfide. The tertiary amine is preferably an optionally substituted trialkyl amine, for example triethylamine or diisopropylamine. A method is also provided in which phosphites or phosphonous esters are sulfurized to their corresponding phosphorothioates or phosphonothioates, respectively, using compositions of the present invention. This method has particular application to produce internucleotide phosphorothioate or phosphonothioate bonds in a nucleotide multimer.
摘要翻译： 适用于有机亚磷酸盐硫化的组合物。 试剂包括硫和叔胺的溶液，在合适的溶剂如二硫化碳中。 叔胺优选为任选取代的三烷基胺，例如三乙胺或二异丙胺。 还提供了使用本发明的组合物分别将亚磷酸酯或膦酸酯硫化成其相应的硫代磷酸酯或膦酰硫酸酯的方法。 该方法特别适用于在核苷酸多聚体中产生核苷酸硫代磷酸酯或硫代磷酸酯键。
-
公开(公告)号：US09212977B2
公开(公告)日：2015-12-15
申请号：US13525225
申请日：2012-06-15
申请人： Zhongliang Tang , Pavel Tsinberg , Ram S. Bhatt
发明人： Zhongliang Tang , Pavel Tsinberg , Ram S. Bhatt
CPC分类号： G01N1/34 , B01F5/102 , B01F13/0059 , B01L3/502753 , B01L3/502761 , B01L2300/0681 , B01L2300/0816 , B01L2300/088 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/086 , G01N1/40 , G01N1/405 , G01N33/54366
摘要： A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流量装置（11,71）使用流线，其中直线流被横向柱（23,81）的图案中断。 所述柱在所述流动路径中的扩张的收集室区域（17,75）的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有弯曲的横截面的直线表面，例如。 圆形或泪滴形，并且随机布置以便破坏流线型流。 该装置被定向成使得其下表面与水平面成约45°对准。 通过亲水涂层，优选含有异氰酸酯部分的渗透性水凝胶连接到收集区域的表面上的螯合剂如Abs在捕获细胞或其它靶向生物分子中而其余液体样品水平离开时是非常有效的。
-
公开(公告)号：US07439062B2
公开(公告)日：2008-10-21
申请号：US11021304
申请日：2004-12-23
申请人： Ram S. Bhatt , Pavel Tsinberg
发明人： Ram S. Bhatt , Pavel Tsinberg
CPC分类号： A61K9/1641 , C12N2533/30
摘要： Spheroidal beads present an exterior surface of a hydrophilic hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents present at the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound. These beads allow sequestering agents to retain their native three-dimensional configuration, and as a result of such surface characteristics and hydrophilicity, they achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.
摘要翻译： 球形珠呈现亲水性水凝胶的外表面，其是通过氨基甲酸酯键聚合并通过氨基甲酸酯和脲键交联的异氰酸酯官能聚合物。 存在于表面的螯合剂共价结合到异氰酸酯基团或与其结合的中间接头。 这些珠子允许螯合剂保留其天然三维构型，并且由于这些表面特性和亲水性，它们实现了非常有效地捕获来自体液等的非常小的稀有细胞亚群，并且非常有效地阻止非特异性结合 存在于这种体液中的其它生物材料。 它们可以是全水凝胶球状体或水凝胶涂覆的基底。
-
公开(公告)号：US20130121895A1
公开(公告)日：2013-05-16
申请号：US13525225
申请日：2012-06-15
申请人： Zhongliang TANG , Pavel TSINBERG , Ram S. BHATT
发明人： Zhongliang TANG , Pavel TSINBERG , Ram S. BHATT
IPC分类号： G01N1/34
CPC分类号： G01N1/34 , B01F5/102 , B01F13/0059 , B01L3/502753 , B01L3/502761 , B01L2300/0681 , B01L2300/0816 , B01L2300/088 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/086 , G01N1/40 , G01N1/405 , G01N33/54366
摘要： A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流量装置（11,71）使用流线，其中直线流被横向柱（23,81）的图案中断。 所述柱在所述流动路径中的扩张的收集室区域（17,75）的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有弯曲的横截面的直线表面，例如。 圆形或泪滴形，并且随机布置以便破坏流线型流。 该装置被定向成使得其下表面与水平面成约45°对准。 通过亲水涂层，优选含有异氰酸酯部分的渗透性水凝胶连接到收集区域的表面上的螯合剂如Abs在捕获细胞或其它靶向生物分子中而其余液体样品水平离开时是非常有效的。
-
公开(公告)号：US20100092470A1
公开(公告)日：2010-04-15
申请号：US12563330
申请日：2009-09-21
申请人： Ram S. Bhatt , Rishi S. Bhatt , Yu Zhang
发明人： Ram S. Bhatt , Rishi S. Bhatt , Yu Zhang
IPC分类号： A61K39/395 , C07K16/00 , C07H21/00 , C12P21/00 , G01N33/566 , G01N33/574 , A61P35/00
CPC分类号： C07K16/00 , C07K2317/22 , Y02A50/58 , Y02A50/59
摘要： Camelid and shark heavy chain only antibodies and their analogs are disclosed. Methods of making such antibodies and their analogs are also provided. Also provided are kits, and methods of using such antibodies and their analogs in diagnostics, prognostics, therapy, and simultaneous diagnosis and therapy.
摘要翻译： 骆驼和鲨鱼重链仅有抗体及其类似物被公开。 还提供制备此类抗体及其类似物的方法。 还提供了试剂盒和在诊断，预后，治疗和同时诊断和治疗中使用这些抗体及其类似物的方法。
-
公开(公告)号：US06031091A
公开(公告)日：2000-02-29
申请号：US908535
申请日：1997-08-07
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这种试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者各自具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
-
9.发明申请RECOVERY OF RARE CELLS USING A MICROCHANNEL APPARATUS WITH PATTERNED POSTS 审中-公开使用具有图案位置的微通道装置恢复稀有细胞公开(公告)号：US20120258475A1
公开(公告)日：2012-10-11
申请号：US13441703
申请日：2012-04-06
申请人： Zhongliang TANG , Ram S. BHATT , Pavel TSINBERG
发明人： Zhongliang TANG , Ram S. BHATT , Pavel TSINBERG
IPC分类号： G01N33/569 , G01N33/53 , G01N33/574 , G01N33/566 , C12M1/00 , C12N5/09 , C12N7/02 , G01N21/75 , G01N1/40 , C12M1/34 , C12N5/073
CPC分类号： G01N1/40 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , C12M47/04 , G01N1/405 , G01N33/53 , G01N33/574
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。
-
公开(公告)号：US20090136982A1
公开(公告)日：2009-05-28
申请号：US11814276
申请日：2006-01-05
申请人： Zhongliang Tang , Pavel Tsinberg , Ram S. Bhatt
发明人： Zhongliang Tang , Pavel Tsinberg , Ram S. Bhatt
CPC分类号： G01N1/34 , B01F5/102 , B01F13/0059 , B01L3/502753 , B01L3/502761 , B01L2300/0681 , B01L2300/0816 , B01L2300/088 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/086 , G01N1/40 , G01N1/405 , G01N33/54366
摘要： A micro now device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof: they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微现在装置（11,71）使用流线，其中直线流被横向柱（23,81）的图案中断。 柱在流动路径中的扩张的收集室区域（17,75）的宽度上间隔开，在其上表面和下表面之间延伸：它们具有直线表面，其横截面弯曲，例如。 圆形或泪滴形，并且随机布置以便破坏流线型流。 该装置被定向成使得其下表面与水平面成约45°对准。 通过亲水涂层，优选含有异氰酸酯部分的渗透性水凝胶连接到收集区域的表面上的螯合剂如Abs在捕获细胞或其它靶向生物分子中而其余液体样品水平离开时是非常有效的。
-
-
-
-
-
-
-
-
-
搜索结果
16 条记录 (耗时 0.02 秒)
