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公开(公告)号：US06031091A
公开(公告)日：2000-02-29
申请号：US908535
申请日：1997-08-07
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这种试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者各自具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
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公开(公告)号：US5585481A
公开(公告)日：1996-12-17
申请号：US182666
申请日：1994-01-14
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这种试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者各自具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
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公开(公告)号：US5696251A
公开(公告)日：1997-12-09
申请号：US485629
申请日：1995-06-07
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这样的试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者都具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
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公开(公告)号：US5656744A
公开(公告)日：1997-08-12
申请号：US490109
申请日：1995-06-07
CPC分类号： C07H21/00 , C07F9/2408
摘要： A versatile reagent with a non-nucleotide monomeric unit having a ligand, and first and second coupling groups which are linked to the non-nucleotide monomeric unit. The ligand can be either a chemical moiety, such as a label or intercalator, or a linking arm which can be linked to such a moiety. Such reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
摘要翻译： 具有具有配体的非核苷酸单体单元的多功能试剂和与非核苷酸单体单元连接的第一和第二偶联基团。 配体可以是化学部分，例如标记或嵌入剂，或可以连接到这种部分的连接臂。 这种试剂允许制备通用的核苷酸/非核苷酸聚合物，具有任何所需的核苷酸和非核苷酸单体单元序列，后者各自具有所需的配体。 这些聚合物可以例如用作可以显示增强的灵敏度的探针和/或能够检测核苷酸属的核苷酸，其中每个物种具有由不感兴趣的不同序列桥接的共同目标核苷酸序列。
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公开(公告)号：US5837856A
公开(公告)日：1998-11-17
申请号：US814053
申请日：1997-03-06
IPC分类号： C12N15/09 , A61K31/70 , A61K48/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/1131 , C07H21/00
摘要： Oligomers having phosphonate internucleosidyl linkages which are enriched for phosphonate linkages of a preselected chirality which hybridize to an RNA target sequence and methods for their preparation are provided.
摘要翻译： 提供了具有与RNA靶序列杂交的预选手性的富含膦酸酯键合的膦酸酯核苷间键的低聚物及其制备方法。
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6.发明授权Polycationic supports and nucleic acid purification separation and hybridization 失效用于核酸纯化，分离和杂交的聚阳离子载体
公开(公告)号：US5599667A
公开(公告)日：1997-02-04
申请号：US311289
申请日：1994-09-23
CPC分类号： C07H21/00 , C07H1/06 , C12Q1/6806 , C12Q1/6834 , C12Q1/689
摘要： Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without significant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.
摘要翻译： 本文描述的是使用聚阳离子固体载体从含有污染物的溶液中纯化核酸。 核酸与载体非共价结合，而不会使污染物与污染物分离而明显结合污染物。 结合的核酸可以从载体中回收。 还描述了使用载体作为用来自未杂交的探针的核苷酸探针分离多核苷酸和其杂交体的手段。 描述了使用这种分离方法的靶核苷酸序列的测定。
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7.发明授权Method for inhibiting RNA translation using chimeric oligonucleoside compounds 失效使用嵌合寡核苷酸化合物抑制RNA翻译的方法公开(公告)号：US06262036B1
公开(公告)日：2001-07-17
申请号：US09490774
申请日：2000-01-24
IPC分类号： A01N6100
CPC分类号： C12N15/1131 , A61K38/00 , A61K48/00 , C07H21/00
摘要： Chimeric oligonucleoside compounds, and methods of preparing and formulating the same, are disclosed. The compounds and compositions are useful in activating RNaseH-mediated cleavage of target ribonucleic acid sequences, and in treating disease conditions relating to such sequences.
摘要翻译： 公开了嵌合寡核苷酸化合物及其制备和配制方法。 化合物和组合物可用于激活核糖核酸酶-H介导的靶核糖核酸序列的切割，以及用于治疗与这些序列有关的疾病状况。
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公开(公告)号：US06316608B1
公开(公告)日：2001-11-13
申请号：US09532656
申请日：2000-03-20
IPC分类号： C07H2104
CPC分类号： C12Q1/6837 , C12Q2545/114
摘要： Methods are provide for determining the relative amounts of individual polynucleotides in a complex mixture. The polynucleotides, after fluorescent labeling, are contacted under hybridization conditions with an array having element disposed at discrete locations on a substrate. The elements comprise two or more distinct polynucleotides that are combined prior to arraying. The level of fluorescence associated with each element provides a measure of its relative amount in the mixture.
摘要翻译： 提供了确定复合混合物中各个多核苷酸的相对量的方法。 在荧光标记之后，多核苷酸在杂交条件下与具有设置在基底上离散位置的元件的阵列接触。 这些元件包括在排列之前组合的两个或更多个不同的多核苷酸。 与每个元素相关的荧光水平提供了其在混合物中的相对量的量度。
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公开(公告)号：US6060456A
公开(公告)日：2000-05-09
申请号：US960111
申请日：1997-10-27
CPC分类号： C12N15/1131 , A61K48/00 , C07H21/00 , A61K38/00
摘要： Chimeric oligonucleoside compounds, and methods of preparing and formulating the same, are disclosed. The compounds and compositions are useful in activating RNaseH-mediated cleavage of target ribonucleic acid sequences, and in treating disease conditions relating to such sequences.
摘要翻译： 公开了嵌合寡核苷酸化合物及其制备和配制方法。 化合物和组合物可用于激活核糖核酸酶-H介导的靶核糖核酸序列的切割，以及用于治疗与这些序列有关的疾病状况。
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公开(公告)号：US5854410A
公开(公告)日：1998-12-29
申请号：US223355
申请日：1994-03-31
IPC分类号： C07H21/00 , C12N15/113 , C07H21/04 , C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C12N2310/3125 , C12N2310/334 , C12N2310/3511 , C12N2310/3515 , C12N2310/53
摘要： Methods and compounds for selective cleavage of nucleic acid are described. The compounds generally contain three functionalities: (1) an oligonucleoside portion which is substantially complementary to at least a portion of the target nucleic acid, thereby providing selectivity to the compound; (2) a non-complementary portion which replaces one of the otherwise-complementary nucleoside bases in the oligonucleoside and which serves to place the target nucleic acid strand into a conformation that favors the cleavage of a phosphodiester linkage opposite the non-complementary portion; and (3) a cleavage moiety which possesses one or more and preferably two or more of the features of (a) proton donation, (b) proton acceptance, (c) hydrogen bonding, (d) charge neutralization and (e) Lewis acidity. These compounds may be used for the study and treatment of diseases involving foreign genetic materials or alterations to or inappropriate expression of genomic DNA.
摘要翻译： 描述了用于选择性切割核酸的方法和化合物。 化合物通常含有三个官能团：（1）寡核苷酸部分，其与目标核酸的至少一部分基本上互补，从而为化合物提供选择性; （2）替代寡核苷酸之一的其它互补核苷碱基的非互补部分，其用于将靶核酸链置于有利于与非互补部分相对的磷酸二酯键的切割的构象; （3）具有（a）质子供体，（b）质子接受，（c）氢键，（d）电荷中和和（e）路易斯酸度的一个或多个，优选两个或多个特征的切割部分 。 这些化合物可用于研究和治疗涉及外来遗传物质的疾病或基因组DNA的改变或不恰当的表达。
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