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公开(公告)号：US20130121895A1
公开(公告)日：2013-05-16
申请号：US13525225
申请日：2012-06-15
申请人： Zhongliang TANG , Pavel TSINBERG , Ram S. BHATT
发明人： Zhongliang TANG , Pavel TSINBERG , Ram S. BHATT
IPC分类号： G01N1/34
CPC分类号： G01N1/34 , B01F5/102 , B01F13/0059 , B01L3/502753 , B01L3/502761 , B01L2300/0681 , B01L2300/0816 , B01L2300/088 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/086 , G01N1/40 , G01N1/405 , G01N33/54366
摘要： A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流量装置（11,71）使用流线，其中直线流被横向柱（23,81）的图案中断。 所述柱在所述流动路径中的扩张的收集室区域（17,75）的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有弯曲的横截面的直线表面，例如。 圆形或泪滴形，并且随机布置以便破坏流线型流。 该装置被定向成使得其下表面与水平面成约45°对准。 通过亲水涂层，优选含有异氰酸酯部分的渗透性水凝胶连接到收集区域的表面上的螯合剂如Abs在捕获细胞或其它靶向生物分子中而其余液体样品水平离开时是非常有效的。
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公开(公告)号：US07695956B2
公开(公告)日：2010-04-13
申请号：US11331988
申请日：2006-01-12
申请人： Pavel Tsinberg , Zhongliang Tang
发明人： Pavel Tsinberg , Zhongliang Tang
IPC分类号： C12M3/00
CPC分类号： G01N1/40 , B01L3/5027 , G01N1/2813
摘要： A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流动路径，其中直线流被横向柱的图案中断，所述横向柱横跨收集区域的宽度以不规则或设置的随机图案 以便破坏流线型流程。 螯合剂如Abs通过亲水可渗透的水凝胶涂层附着在收集区域中的所有表面上。 收集区域形成为由PDMS成型的主体中的空腔，该柔性体被夹在玻璃滑块或类似的平板和刚性顶盖板之间，两者都通过热量与PDMS体压接成邻接关系 -shrunk聚合物套管。 细胞分离和洗涤后，细胞可以从多价螯合剂中释放出来，离心的装置迫使细胞收集在水凝胶涂层的载玻片或板附近。 分开聚合物套筒可以使用整体制表片将身体从滑块或板上剥离，以暴露其顶部表面上的分离的细胞，以进行显微镜检查。
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公开(公告)号：US20100004578A1
公开(公告)日：2010-01-07
申请号：US12499038
申请日：2009-07-07
IPC分类号： A61M1/14
CPC分类号： A61M1/14 , A61M2206/11 , B01D11/0288 , B01D11/0492 , B01D11/0496 , B01L3/5027
摘要： The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety of situations where a sample fluid is to be purified via a diffusion mechanism against an extractor fluid. Moreover, the invention may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of small molecules, middle molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
摘要翻译： 本发明涉及通过与第二流体接触从样品流体中除去不需要的材料的装置，系统和方法。 样品流体作为与第二流体的同时流动的层相邻或相邻的薄层流动，而没有中间膜。 在各种实施方案中，使用次级分离器来限制所需物质的去除并且实现从血液中除去不需要的物质。 本发明可用于各种情况，其中样品液体经由针对抽提流体的扩散机制进行纯化。 此外，本发明可用于从尺寸变化的样品流体中除去组分。 例如，当血液是样品液体时，这可能包括从血液样品到提取液中去除小分子，中分子，大分子，大分子聚集体和细胞。
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公开(公告)号：US08083706B2
公开(公告)日：2011-12-27
申请号：US12499038
申请日：2009-07-07
IPC分类号： A61M37/00 , A61M29/00 , G10N21/00 , G01N31/00 , G01N33/00 , B01D21/24 , B01D25/30 , B01D33/70 , B01D11/00 , B01D12/00
CPC分类号： A61M1/14 , A61M2206/11 , B01D11/0288 , B01D11/0492 , B01D11/0496 , B01L3/5027
摘要： The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety of situations where a sample fluid is to be purified via a diffusion mechanism against an extractor fluid. Moreover, the invention may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of ‘small’ molecules, ‘middle’ molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
摘要翻译： 本发明涉及通过与第二流体接触从样品流体中除去不需要的材料的装置，系统和方法。 样品流体作为与第二流体的同时流动的层相邻或相邻的薄层流动，而没有中间膜。 在各种实施方案中，使用次级分离器来限制所需物质的去除并且实现从血液中除去不需要的物质。 本发明可用于各种情况，其中样品液体经由针对抽提流体的扩散机制进行纯化。 此外，本发明可用于从尺寸变化的样品流体中除去组分。 当血液是样品液体时，例如，这可能包括从血液样品到提取液中去除“小”分子，“中”分子，大分子，大分子聚集体和细胞。
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公开(公告)号：US08076127B2
公开(公告)日：2011-12-13
申请号：US11038920
申请日：2005-01-18
申请人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
发明人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
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6.发明授权Systems and methods of blood-based therapies having a microfluidic membraneless exchange device 失效具有微流体无膜交换装置的血液疗法的系统和方法公开(公告)号：US07850633B2
公开(公告)日：2010-12-14
申请号：US12509395
申请日：2009-07-24
CPC分类号： A61M1/14 , A61M1/1696 , A61M1/32 , A61M1/3482 , A61M1/3621 , A61M2206/11 , B01D11/04 , B01D11/0496 , B01D61/243 , B01D61/28 , B01L3/5027 , G01N2001/4016 , Y10T436/255
摘要： The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety of situations where a sample fluid is to be purified via a diffusion mechanism against an extractor fluid. Moreover, the invention may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of ‘small’ molecules, ‘middle’ molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
摘要翻译： 本发明涉及通过与第二流体接触从样品流体中除去不需要的材料的装置，系统和方法。 样品流体作为与第二流体的同时流动的层相邻或相邻的薄层流动，而没有中间膜。 在各种实施方案中，使用次级分离器来限制所需物质的去除并且实现从血液中除去不需要的物质。 本发明可用于各种情况，其中样品液体经由针对抽提流体的扩散机制进行纯化。 此外，本发明可用于从尺寸变化的样品流体中除去组分。 当血液是样品液体时，例如，这可能包括从血液样品到提取液中去除“小”分子，“中”分子，大分子，大分子聚集体和细胞。
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7.发明授权公开(公告)号：US08158410B2
公开(公告)日：2012-04-17
申请号：US11038920
申请日：2005-01-18
申请人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
发明人： Zhongliang Tang , Ram S. Bhatt , Pavel Tsinberg
CPC分类号： G01N1/40 , G01N1/405 , Y10T436/25375
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。
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8.发明申请公开(公告)号：US20110056884A1
公开(公告)日：2011-03-10
申请号：US12942519
申请日：2010-11-09
IPC分类号： B01D61/26
CPC分类号： A61M1/14 , A61M1/1696 , A61M1/32 , A61M1/3482 , A61M1/3621 , A61M2206/11 , B01D11/04 , B01D11/0496 , B01D61/243 , B01D61/28 , B01L3/5027 , G01N2001/4016 , Y10T436/255
摘要： The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety of situations where a sample fluid is to be purified via a diffusion mechanism against an extractor fluid. Moreover, the invention may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of ‘small’ molecules, ‘middle’ molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
摘要翻译： 本发明涉及通过与第二流体接触从样品流体中除去不需要的材料的装置，系统和方法。 样品流体作为与第二流体的同时流动的层相邻或相邻的薄层流动，而没有中间膜。 在各种实施方案中，使用次级分离器来限制所需物质的去除并且实现从血液中除去不需要的物质。 本发明可用于各种情况，其中样品液体经由针对抽提流体的扩散机制进行纯化。 此外，本发明可用于从尺寸变化的样品流体中除去组分。 当血液是样品液体时，例如，这可能包括从血液样品到提取液中去除“小”分子，“中”分子，大分子，大分子聚集体和细胞。
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公开(公告)号：US08491516B2
公开(公告)日：2013-07-23
申请号：US13298344
申请日：2011-11-17
CPC分类号： A61M1/14 , A61M2206/11 , B01D11/0288 , B01D11/0492 , B01D11/0496 , B01L3/5027
摘要： Devices, systems and methods are disclosed for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The embodiments may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of small molecules, middle molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
摘要翻译： 公开了用于通过与第二流体接触从样品流体中除去不需要的材料的装置，系统和方法。 样品流体作为与第二流体的同时流动的层相邻或相邻的薄层流动，而没有中间膜。 在各种实施方案中，使用次级分离器来限制所需物质的去除并且实现从血液中除去不需要的物质。 这些实施例可用于从尺寸变化的样品流体中去除组分。 例如，当血液是样品液体时，这可能包括从血液样品到提取液中去除小分子，中分子，大分子，大分子聚集体和细胞。
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10.发明申请RECOVERY OF RARE CELLS USING A MICROCHANNEL APPARATUS WITH PATTERNED POSTS 审中-公开使用具有图案位置的微通道装置恢复稀有细胞公开(公告)号：US20120258475A1
公开(公告)日：2012-10-11
申请号：US13441703
申请日：2012-04-06
申请人： Zhongliang TANG , Ram S. BHATT , Pavel TSINBERG
发明人： Zhongliang TANG , Ram S. BHATT , Pavel TSINBERG
IPC分类号： G01N33/569 , G01N33/53 , G01N33/574 , G01N33/566 , C12M1/00 , C12N5/09 , C12N7/02 , G01N21/75 , G01N1/40 , C12M1/34 , C12N5/073
CPC分类号： G01N1/40 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , C12M47/04 , G01N1/405 , G01N33/53 , G01N33/574
摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。
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