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    Compositions and methods for use in analytical reactions
    3.
    发明授权
    Compositions and methods for use in analytical reactions 有权
    用于分析反应的组合物和方法

    公开(公告)号：US08252911B2

    公开(公告)日：2012-08-28

    申请号：US12370472

    申请日：2009-02-12

    Applicant: Keith Bjornson ,  Arek Bibillo ,  Lubomir Sebo

    Inventor： Keith Bjornson ,  Arek Bibillo ,  Lubomir Sebo

    IPC: C07H21/02 ,  C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6874 ,  C12Q2527/127 ,  C12Q2527/113

    Abstract: Compositions, methods, substrates and systems for use in analysis of single molecule reactions and particularly single molecule nucleic acid sequence analysis. Compositions that include non-reactive, distinguishable or undetectable competitive substrates for the reaction system of interest are provided, as well as their use in systems and substrates for such applications, such compounds typically preferably polyphosphate chains or analogous structures.

    Abstract translation: 用于分析单分子反应，特别是单分子核酸序列分析的组合物，方法，底物和系统。 提供了包含用于感兴趣的反应体系的非反应性，可区分或不可检测的竞争性底物的组合物，以及它们在用于这些应用的系统和底物中的用途，这些化合物通常优选多磷酸酯链或类似结构。

    SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS
    6.
    发明申请
    SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS 有权
    单分子序列与两个不同的化学步骤

    公开(公告)号：US20110212437A1

    公开(公告)日：2011-09-01

    申请号：US13022926

    申请日：2011-02-08

    Applicant: Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    Inventor： Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    IPC: C12Q1/68 ,  C12M1/34 ,  C07H19/04

    CPC classification number: C12Q1/6869 ,  C07H19/10 ,  C07H19/11 ,  C07H19/20 ,  C07H19/213 ,  C12Q1/6874 ,  C12Q2521/301 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2537/157 ,  C12Q2521/319 ,  C12Q2521/525

    Abstract: Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

    Abstract translation: 提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。 在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。 选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。 在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。 具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。

    Single molecule sequencing with two distinct chemistry steps
    8.
    发明授权
    Single molecule sequencing with two distinct chemistry steps 有权
    具有两个不同化学步骤的单分子测序

    公开(公告)号：US08603741B2

    公开(公告)日：2013-12-10

    申请号：US13022926

    申请日：2011-02-08

    Applicant: Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    Inventor： Robin Emig ,  Lei Jia ,  Jeremiah Hanes ,  Lubomir Sebo

    IPC: C12Q1/68 ,  C07H19/04

    CPC classification number: C12Q1/6869 ,  C07H19/10 ,  C07H19/11 ,  C07H19/20 ,  C07H19/213 ,  C12Q1/6874 ,  C12Q2521/301 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2537/157 ,  C12Q2521/319 ,  C12Q2521/525

    Abstract: Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

    Abstract translation: 提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。 在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。 选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。 在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。 具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。

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