Abstract:
Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
Abstract:
An apparatus for detecting a gamma-ray includes: a gamma-ray detection material comprising a material transparent to light having a plurality of nano-crystallites where each nano-crystallite in the plurality has as periodic crystal structure with a diameter or dimension that is less than 1000 nm and includes (i) a heavy atom having an atomic number greater than or equal to 55 that emits an energetic electron upon interacting with an incoming gamma-ray and (ii) and an activator atom that provides for scintillation upon interacting with the energetic electron to emit light photons wherein the heavy atom and the activator atom have positions in the periodic crystal structure of each nano-crystallite in the plurality; and a photodetector optically coupled to the gamma-ray detection material and configured to detect the light photons emitted from the scintillation and to provide a signal correlated to the detected light photons.
Abstract:
Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
Abstract:
Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Abstract:
An apparatus for providing a signal indicative of a property of an earth formation includes: a carrier conveyable through a borehole; a neutron source disposed on the carrier and configured to emit neutrons into the earth formation; a radiation detector disposed on the carrier and configured to detect radiation from the earth formation due to interaction of emitted neutrons with the earth formation and to provide the signal indicative of the property; and a neutron shield configured to shield the radiation detector from emitted neutrons that did not interact with the earth formation; wherein the radiation detector shield includes a glass ceramic material having a plurality of nano-crystallites, each nano-crystallite in the plurality having a periodic crystal structure with a diameter or dimension that is less than 1000 nm that includes Li and/or Boron and a rare-earth element that have positions in the periodic crystal structure of each nano-crystallite.
Abstract:
Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Abstract:
The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
Abstract:
Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.