Compositions and methods for detection and isolation of phosphorylated molecules
    62.
    发明申请
    Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
    用于磷酸化分子检测和分离的组合物和方法

    公开(公告)号:US20100009381A1

    公开(公告)日:2010-01-14

    申请号:US12500854

    申请日:2009-07-10

    IPC分类号: G01N33/53

    摘要: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

    摘要翻译: 本发明涉及用于结合,检测和分离磷酸化靶分子的磷酸盐结合化合物,包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物,酸和金属离子,其中金属离子同时与靶分子上暴露的磷酸基团相互作用,并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子,从复杂混合物中分离磷酸化靶分子,并辅助蛋白质组分析,其中可以鉴定激酶和磷酸酶底物和酶。

    FLUOROGENIC PROTEIN KINASE SUBSTRATES
    63.
    发明申请
    FLUOROGENIC PROTEIN KINASE SUBSTRATES 有权
    荧光蛋白激酶基质

    公开(公告)号:US20080009026A1

    公开(公告)日:2008-01-10

    申请号:US11774554

    申请日:2007-07-06

    申请人: Kyle Gee

    发明人: Kyle Gee

    IPC分类号: C12Q1/48

    CPC分类号: C12Q1/485 G01N33/573

    摘要: The present invention relates to kinase sensors comprising a metal chelator and a fluorophore, where the chelator comprises a quinoline group and where the fluorophore is conjugated to the chelator. The invention also relates to methods of using these kinase sensors as well as kits comprising the kinase sensors.

    摘要翻译: 本发明涉及包含金属螯合剂和荧光团的激酶传感器,其中螯合剂包含喹啉基团,其中荧光团与螯合剂结合。 本发明还涉及使用这些激酶传感器的方法以及包含激酶传感器的试剂盒。

    Competitive immunoassay
    65.
    发明申请
    Competitive immunoassay 有权
    竞争性免疫测定

    公开(公告)号:US20060160068A1

    公开(公告)日:2006-07-20

    申请号:US10943463

    申请日:2004-09-17

    摘要: The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.

    摘要翻译: 本发明提供配体检测试剂,配体类似物和用于测定样品中配体存在的方法。 配体检测试剂包含配体结合抗体和配体类似物以形成抗体 - 配体类似物复合物,其中配体类似物共价键合到报告分子。 该复合物还可以包含与抗体非共价键合以形成三元复合物的标记蛋白质,其中标记蛋白质包含共价键合到标记部分的单价抗体片段或非抗体蛋白质。 取决于报道分子和配体结合抗体,报告分子被配体结合抗体或标记蛋白的标记部分淬灭,其中淬灭量与存在于 样品。 或者,配体类似物是荧光的,其中配体类似物在溶液中基本上是非荧光的,但当被配体结合抗体结合时,可检测信号增加。 在这种情况下,与目标配体的存在相比,测量了与消除淬灭相反的信号降低。