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公开(公告)号：US12029528B2
公开(公告)日：2024-07-09
申请号：US16626900
申请日：2018-06-25
Applicant: FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
Inventor： Pascal Kopperschmidt
CPC classification number: A61B5/0071 , A61B5/4848 , A61K49/0039 , A61L2/0088 , A61L2/28 , A61M1/169 , A61L2202/15 , A61M2205/3313
Abstract: The present invention relates to a method of disinfecting a region, in particular a skin region of a patient, wherein the method comprises the following steps: a) Application of an optically effective substance to the region; and b) Application of a disinfectant to the region, wherein the optically effective substance and the disinfectant are configured such that the optically effective substance is removed by the disinfectant or its optical properties are changed by the disinfectant.
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公开(公告)号：US11931428B2
公开(公告)日：2024-03-19
申请号：US16947869
申请日：2020-08-21
Applicant: Wake Forest University Health Sciences
Inventor： John C. Lukesh, III
CPC classification number: A61K49/0039 , A61K9/0019 , A61K9/0053 , C07F11/00
Abstract: Provided herein is a compound of formula I:
and the use thereof for detecting the presence of hydrogen sulfide in cells or tissues in vitro or in vivo. The detecting may be useful, for example, in diagnosing cancer or other diseases related to imbalanced hydrogen sulfide (H2S) production such as neurodegenerative diseases.
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公开(公告)号：US20180127830A1
公开(公告)日：2018-05-10
申请号：US15567050
申请日：2016-04-14
Applicant: CASE WESTERN RESERVE UIVERSITY
Inventor： Stanton Gerson , Yanming Wang , Allison G. Condit
IPC: C12Q1/6886 , A61K49/00 , C12Q1/6827
CPC classification number: C12Q1/6886 , A61K49/0019 , A61K49/0032 , A61K49/0039 , C12Q1/6827 , C12Q2600/136 , C12Q2525/119 , C12Q2563/107
Abstract: A fluorescent probe for binding to and detection of AP sites of DNA includes the following formula: F-L-X where F is a fluorescent moiety, X is an aminooxy group (—ONH2), and L is a linker that links or couples the fluorescent moiety to the oxyamine.
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公开(公告)号：US20170355730A1
公开(公告)日：2017-12-14
申请号：US15360719
申请日：2016-11-23
Applicant: CYCLOPORTERS, INC.
Inventor： Dehua PEI , Ziqing QIAN
CPC classification number: C07K7/64 , A61K38/05 , A61K38/12 , A61K38/465 , A61K47/64 , A61K49/0039 , A61K49/0041 , A61K49/0043 , A61K49/0047 , A61K49/0056 , C07K7/06 , C12Y301/03048
Abstract: Disclosed herein are compounds having activity as cell penetrating peptides. In some examples, the compounds can comprise a cell penetrating peptide moiety and a cargo moiety. The cargo moiety can comprise one or more detectable moieties, one or more therapeutic moieties, one or more targeting moieties, or any combination thereof. In some examples, the cell penetrating peptide moiety is cyclic. In some examples, the cell penetrating peptide moiety and cargo moiety together are cyclic. In some examples, the cell penetrating peptide moiety is cyclic and the cargo moiety is appended to the cyclic cell penetrating peptide moiety structure. In some examples, the cargo moiety is cyclic and the cell penetrating peptide moiety is cyclic, and together they form a fused bicyclic system.
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5.发明申请
公开(公告)号：US20170122955A1
公开(公告)日：2017-05-04
申请号：US15302782
申请日：2015-04-06
Applicant: HIROSAKI UNIVERSITY
Inventor： Katsuya YAMADA , Ayako SASAKI , Tadashi TESHIMA , Toshihiro YAMAMOTO , Yuji OTSUKA
IPC: G01N33/66 , G01N33/58 , C07H17/02 , C07H17/075 , G01N33/574
CPC classification number: G01N33/66 , A61K49/0002 , A61K49/0021 , A61K49/0039 , A61K49/0052 , A61K51/0491 , C07H17/02 , C07H17/075 , C07H19/04 , G01N33/574 , G01N33/582
Abstract: Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided are a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.
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6.发明申请NON-INVASIVE ENERGY UPCONVERSION METHODS AND SYSTEMS FOR IN-SITU PHOTOBIOMODULATION 审中-公开非入侵能源逆转方法和系统用于现场光电转换
公开(公告)号：US20160263393A1
公开(公告)日：2016-09-15
申请号：US15045524
申请日：2016-02-17
Applicant: IMMUNOLIGHT, LLC , DUKE UNIVERSITY
Inventor： Tuan VO-DINH , Jonathan P. SCAFFIDI , Venkata Gopal Reddy CHADA , Benoit LAULY , Yan ZHANG , Molly K. GREGAS , Ian Nicholas STANTON , Joshua T. STECHER , Michael J. THERIEN , Frederic A. BOURKE, JR. , Harold WALDER , Zak FATHI , Jennifer A. AYRES , Zhenyuan ZHANG , Joseph H. SIMMONS , Stephen John NORTON
IPC: A61N5/06 , A61N1/40 , A61N5/10 , A61K41/00 , A61M5/00 , A61B6/00 , A61B5/055 , A61N5/02 , A61B5/00
CPC classification number: A61N5/062 , A61B5/0059 , A61B5/0071 , A61B5/055 , A61B5/4848 , A61B6/481 , A61K41/0042 , A61K41/0052 , A61K41/0061 , A61K49/0039 , A61L2/0047 , A61L2/08 , A61L2202/21 , A61L2202/22 , A61M5/007 , A61N1/406 , A61N5/02 , A61N5/0601 , A61N5/0618 , A61N5/0622 , A61N5/0624 , A61N5/10 , A61N2005/063 , A61N2005/0656 , A61N2005/0659 , A61N2005/0662 , A61N2005/067 , A61N2005/1098
Abstract: Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, including treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by placing a nanoparticle having a metallic shell on at least a fraction of a surface in a vicinity of a target structure in a subject and applying an initiation energy to a subject thus producing an effect on or change to the target structure directly or via a modulation agent. The nanoparticle is configured, upon exposure to a first wavelength λ1, to generate a second wavelength λ2 of radiation having a higher energy than the first wavelength λ1. The methods may further be performed by application of an initiation energy to a subject in situ to activate a pharmaceutical agent directly or via an energy modulation agent, optionally in the presence of one or more plasmonics active agents, thus producing an effect on or change to the target structure. Kits containing products or compositions formulated or configured and systems for use in practicing these methods.
Abstract translation: 用于修饰介导或与生物活性相关的靶结构的产物，组合物，系统和方法，包括治疗由病毒，细胞，亚细胞结构介导或与靶结构相关的疾病，病症或疾病 或胞外结构。 所述方法可以以非侵入性方式在原位进行，通过将具有金属壳的纳米颗粒放置在受试者的靶结构附近的表面的至少一部分上，并向受试者施加引发能量，从而产生 直接或通过调制剂对目标结构的影响或改变。 纳米颗粒在暴露于第一波长λ1时被配置为产生具有比第一波长λ1更高的能量的辐射的第二波长λ2。 所述方法还可以通过向受试者原位施加引发能量来直接或经由能量调节剂活化药剂，任选地在一种或多种等离子体激活剂活性剂的存在下进行，从而产生对其的作用或改变 目标结构。 包含配制或配置的产品或组合的套件以及用于实施这些方法的系统。
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7.发明授权Non-invasive energy upconversion methods and systems for in-situ photobiomodulation 有权用于原位光生物调节的非侵入性能量上转换方法和系统
公开(公告)号：US09302116B2
公开(公告)日：2016-04-05
申请号：US12764184
申请日：2010-04-21
Applicant: Tuan Vo-Dinh , Jonathan P. Scaffidi , Venkata Gopal Reddy Chada , Benoit Lauly , Yan Zhang , Molly K. Gregas , Ian N. Stanton , Joshua T. Stecher , Michael J. Therien , Frederic A. Bourke, Jr. , Harold Walder , Zak Fathi , Jennifer A. Ayres , Zhenyuan Zhang , Joseph H. Simmons , Stephen John Norton
Inventor： Tuan Vo-Dinh , Jonathan P. Scaffidi , Venkata Gopal Reddy Chada , Benoit Lauly , Yan Zhang , Molly K. Gregas , Ian N. Stanton , Joshua T. Stecher , Michael J. Therien , Frederic A. Bourke, Jr. , Harold Walder , Zak Fathi , Jennifer A. Ayres , Zhenyuan Zhang , Joseph H. Simmons , Stephen John Norton
CPC classification number: A61N5/062 , A61B5/0059 , A61B5/0071 , A61B5/055 , A61B5/4848 , A61B6/481 , A61K41/0042 , A61K41/0052 , A61K41/0061 , A61K49/0039 , A61L2/0047 , A61L2/08 , A61L2202/21 , A61L2202/22 , A61M5/007 , A61N1/406 , A61N5/02 , A61N5/0601 , A61N5/0618 , A61N5/0622 , A61N5/0624 , A61N5/10 , A61N2005/063 , A61N2005/0656 , A61N2005/0659 , A61N2005/0662 , A61N2005/067 , A61N2005/1098
Abstract: Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, including treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by placing a nanoparticle having a metallic shell on at least a fraction of a surface in a vicinity of a target structure in a subject and applying an initiation energy to a subject thus producing an effect on or change to the target structure directly or via a modulation agent. The nanoparticle is configured, upon exposure to a first wavelength λ1, to generate a second wavelength λ2 of radiation having a higher energy than the first wavelength λ1. The methods may further be performed by application of an initiation energy to a subject in situ to activate a pharmaceutical agent directly or via an energy modulation agent, optionally in the presence of one or more plasmonics active agents, thus producing an effect on or change to the target structure. Kits containing products or compositions formulated or configured and systems for use in practicing these methods.
Abstract translation: 用于修饰介导或与生物活性相关的靶结构的产物，组合物，系统和方法，包括治疗由病毒，细胞，亚细胞结构介导或与靶结构相关的疾病，病症或疾病 或胞外结构。 所述方法可以以非侵入性方式在原位进行，通过将具有金属壳的纳米颗粒放置在受试者的靶结构附近的表面的至少一部分上，并向受试者施加引发能量，从而产生 直接或通过调制剂对目标结构的影响或改变。 纳米颗粒在暴露于第一波长λ1时被配置为产生具有比第一波长λ1更高的能量的辐射的第二波长λ2。 所述方法还可以通过向受试者原位施加引发能量来直接或经由能量调节剂活化药剂，任选地在一种或多种等离子体激活剂活性剂的存在下进行，从而产生对其的作用或改变 目标结构。 包含配制或配置的产品或组合的套件以及用于实施这些方法的系统。
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公开(公告)号：US09265844B2
公开(公告)日：2016-02-23
申请号：US13990866
申请日：2011-12-01
Applicant: Ching-Hsuan Tung , Wael R. Abd-Elgaliel , Craig D. Logsdon , Zobeida Cruz-Monserrate
Inventor： Ching-Hsuan Tung , Wael R. Abd-Elgaliel , Craig D. Logsdon , Zobeida Cruz-Monserrate
IPC: A61B5/00 , A61B8/00 , A61B10/00 , C12Q1/37 , A61K38/04 , C07K5/00 , C07K7/00 , C07K16/00 , C07K17/00 , A61K49/00 , A61K47/48 , G01N33/533 , G01N33/542 , G01N33/574 , G01N21/64 , C12N15/11 , A61K48/00 , A61K38/00
CPC classification number: A61K49/0045 , A61K38/00 , A61K47/48238 , A61K47/48861 , A61K47/62 , A61K47/6923 , A61K48/00 , A61K49/0039 , A61K49/0056 , C12N15/111 , C12N2310/14 , C12N2320/32 , C12Q1/37 , G01N21/6486 , G01N33/533 , G01N33/542 , G01N33/574 , G01N2800/52
Abstract: Provided herein are polypeptides that are selectively cleaved by cathepsin E. Also provided are methods of detecting cathepsin E. The methods comprise contacting cathepsin E with the polypeptides provided herein and detecting fluorescence. Further provided are methods of diagnosing cancer or pre-cancerous conditions in a subject. Also provided herein is a multilayered nanoparticle or a composition comprising the multilayer nanoparticle, wherein the multilayered nanoparticle comprises a negatively charged nanoparticle core or capsule coated with alternating positive and negative layers. Optionally, the positive layer comprises a positively charged protease degradable polypeptide. Optionally, the negative layer comprises a negatively charged therapeutic agent or a therapeutic agent and a means for providing the agent with a negative charge. For example, optionally, the therapeutic agent is linked to a negatively charged polymer. Further provided are methods of treating or preventing a disease characterized by expression of a protease in a subject using the nanoparticle.
Abstract translation: 本文提供了被组织蛋白酶E选择性切割的多肽。还提供了检测组织蛋白酶E的方法。该方法包括使组织蛋白酶E与本文提供的多肽接触并检测荧光。 还提供了诊断受试者的癌症或癌前病症的方法。 本文还提供了多层纳米颗粒或包含多层纳米颗粒的组合物，其中所述多层纳米颗粒包含涂覆有交替的正层和负层的带负电荷的纳米颗粒核或胶囊。 任选地，阳性层包含带正电荷的蛋白酶降解多肽。 任选地，负层包含带负电的治疗剂或治疗剂和用于向药物提供负电荷的方法。 例如，任选地，治疗剂与带负电荷的聚合物连接。 还提供了治疗或预防使用纳米颗粒在受试者中表达蛋白酶特征的疾病的方法。
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公开(公告)号：US20160025713A1
公开(公告)日：2016-01-28
申请号：US14876782
申请日：2015-10-06
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Courtenay Hart-Kerndt , Wai-Yee Leung , Wayne Patton , Aleksey Rukavishnikov , Richard Haugland , Zhenjun Diwu
IPC: G01N33/52 , C07F5/02 , C07D311/16 , G01N33/58
CPC classification number: G01N33/52 , A61K49/0021 , A61K49/0039 , A61K49/0041 , A61K49/0052 , C07D311/12 , C07D311/16 , C07D311/18 , C07F5/022 , G01N33/533 , G01N33/582
Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.
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10.发明申请
公开(公告)号：US20150241440A1
公开(公告)日：2015-08-27
申请号：US14426599
申请日：2013-09-05
Applicant: UNIVERSITY OF ROCHESTER
Inventor： Rudi Fasan , John R. Frost
IPC: G01N33/58 , C07D495/04 , C12N9/42 , C09B57/02
CPC classification number: C07K1/13 , A61K47/65 , A61K49/0039 , A61K49/0054 , C07C323/58 , C07C323/59 , C07C323/60 , C07D249/04 , C07D311/16 , C07D495/04 , C07K1/1072 , C07K14/35 , C07K2319/20 , C07K2319/21 , C07K2319/50 , C07K2319/60 , C07K2319/92 , C09B57/02 , C12N9/2442 , C12Y302/01014 , G01N33/582 , G01N33/6803
Abstract: Methods and compositions are provided for covalently linking a chemical species to a recombinant or synthetic polypeptide. The methods involve the reaction of a thioester-comprising polypeptide with a reagent comprising a reactive amino-thiol group connected to the chemical species which is to be covalently linked to the polypeptide, via a linker. Such chemical species can be a functional group, a label or tag molecule, a biological molecule, a ligand, or a solid support. Efficient and catalyst-free methods for C-terminal protein labeling are also provided. The methods expand current capabilities in the area of protein functionalization, providing useful and complementary tools for the isolation, detection, characterization, and analysis of proteins in a variety of in vitro and in vivo applications.
Abstract translation: 提供方法和组合物以共价连接化学物质与重组或合成的多肽。 该方法涉及含硫酯的多肽与包含通过连接体共价连接至多肽的化学物质连接的反应性氨基硫醇基团的试剂的反应。 这种化学物质可以是官能团，标记物或标签分子，生物分子，配体或固体支持物。 还提供了有效和无催化剂的C-末端蛋白标记方法。 该方法扩展了蛋白质功能化领域的当前能力，为各种体外和体内应用中蛋白质的分离，检测，表征和分析提供了有用和互补的工具。
