Luminescence quenching compounds
    2.
    发明申请
    Luminescence quenching compounds 有权
    发光淬灭化合物

    公开(公告)号:US20120123109A1

    公开(公告)日:2012-05-17

    申请号:US13374728

    申请日:2012-01-09

    IPC分类号: C07H21/00 C07D409/14

    摘要: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

    摘要翻译: 本发明的淬灭化合物是被一个或多个杂芳族猝灭部分取代的弱发光花青素。 本发明的淬灭化合物表现出很少或不可观察到的发光,并有效地淬灭广谱的发光化合物。 化学反应猝灭化合物具有用于标记各种物质(包括生物分子)的效用。 这些标记的物质对于各种能量转移测定和应用是非常有用的。

    Cyanine dyes and their applications as luminescence quenching compounds
    5.
    发明授权
    Cyanine dyes and their applications as luminescence quenching compounds 有权
    花青染料及其作为发光淬灭剂的应用

    公开(公告)号:US08093411B2

    公开(公告)日:2012-01-10

    申请号:US12807180

    申请日:2010-08-30

    IPC分类号: C07D209/46 C07D209/02

    摘要: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

    摘要翻译: 本发明的淬灭化合物是被一个或多个杂芳族猝灭部分取代的弱发光花青素。 本发明的淬灭化合物表现出很少或不可观察到的发光,并有效地淬灭广谱的发光化合物。 化学反应猝灭化合物具有用于标记各种物质(包括生物分子)的效用。 这些标记的物质对于各种能量转移测定和应用是非常有用的。

    Kits for measuring cellular transmembrane potential changes
    9.
    发明授权
    Kits for measuring cellular transmembrane potential changes 有权
    用于测量细胞跨膜电位变化的试剂盒

    公开(公告)号:US07358064B2

    公开(公告)日:2008-04-15

    申请号:US11020536

    申请日:2004-12-22

    IPC分类号: C12Q1/02

    CPC分类号: G01N33/52 Y10S435/975

    摘要: The invention encompasses kits for an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Devices Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells wherein X is O or S; and n is 1 or 2.

    摘要翻译: 本发明包括用于使用式I化合物作为电位探针测量膜电位的改进方法的试剂盒。 这些探针可以与其它荧光指示剂如Indo-1,Fura-2和Fluo-3组合使用,这样的探针可以用于微孔板读取装置,例如FLIPR TM,荧光成像板读数器,由Molecular 设备公司,加利福尼亚州桑尼维尔市 流式细胞仪 和荧光计。 这样的探针用于测量活细胞中的膜电位,其中X是O或S; n为1或2。

    Detection of transmembrane potentials using N,N,N'-Trialkyl thiobarbituric acid-derived polymethine oxonols
    10.
    发明申请
    Detection of transmembrane potentials using N,N,N'-Trialkyl thiobarbituric acid-derived polymethine oxonols 有权
    使用N,N,N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂环丁烷的跨膜电位检测

    公开(公告)号:US20060088816A1

    公开(公告)日:2006-04-27

    申请号:US11212203

    申请日:2005-08-25

    IPC分类号: C12Q1/00 C07D403/02

    CPC分类号: C07D239/66 G01N33/52

    摘要: The present invention relates generally to the detection and measurement of transmembrane potentials using an N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a slightly hydrophobic N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols. The method comprises a slightly hydrophobic N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

    摘要翻译: 本发明一般涉及使用N,N,N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂多酚(如下所示)检测和测量跨膜电位。 特别地,本发明涉及使用稍微疏水的N,N,N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂多糖测定生物细胞质膜跨膜电位的组合物和光学方法。 该方法包括一种稍微疏水的N,N,N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂氮杂阴离子,其能够响应膜的电位变化而从膜的第一面再分配到膜的第二面。 在一个方面,该方法用于鉴定调节生物膜中膜电位的化合物。 其中R 1,R 2和R 3独立地选自氢,烷基,卤代烷基和杂烷基; n为1〜3的整数, Z是Na,K,铵或其他生物可接受的盐。