知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

68 条记录 (耗时 0.046 秒)

    Modified Hydrocyanine Dyes for the Detection of Reactive Oxygen Species
    3.
    发明申请
    Modified Hydrocyanine Dyes for the Detection of Reactive Oxygen Species 审中-公开
    用于检测活性氧物质的改性水解染料

    公开(公告)号：US20130287689A1

    公开(公告)日：2013-10-31

    申请号：US13881672

    申请日：2011-11-01

    申请人： Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli ,  Lai-Qiang Ying ,  Bruce Branchaud

    发明人： Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli ,  Lai-Qiang Ying ,  Bruce Branchaud

    IPC分类号： G01N21/64 ,  C09B57/00

    CPC分类号： G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

    摘要： The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

    摘要翻译： 本发明涉及通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原染料，包括基于Cy的氢化花青染料和Cy-基氘异氰染料，其染料是用于检测ROS的探针和测量体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

    Compositions and methods for detection and isolation of phosphorylated molecules
    9.
    发明申请
    Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开
    用于磷酸化分子检测和分离的组合物和方法

    公开(公告)号：US20110021372A1

    公开(公告)日：2011-01-27

    申请号：US12821003

    申请日：2010-06-22

    申请人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

    发明人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

    IPC分类号： C40B30/04 ,  C12Q1/68 ,  G01N33/68 ,  G01N33/566

    CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

    摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

    摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。