Near infrared fluorogen and fluorescent activating proteins for in vivo imaging and live-cell biosensing
    1.
    发明授权
    Near infrared fluorogen and fluorescent activating proteins for in vivo imaging and live-cell biosensing 有权
    近红外荧光和荧光激活蛋白,用于体内成像和活细胞生物传感

    公开(公告)号:US09023998B2

    公开(公告)日:2015-05-05

    申请号:US13920775

    申请日:2013-06-18

    摘要: Tissue slices and whole organisms offer substantial challenges to fluorescence imaging. Autofluorescence and absorption via intrinsic chromophores, such as flavins, melanin, and hemoglobins, confound and degrade output from all fluorescent tags. An “optical window,” farther red than most autofluorescence sources and in a region of low hemoglobin and water absorbance, lies between 650 and 900 nm. This valley of relative optical clarity is an attractive target for fluorescence-based studies within tissues, intact organs, and living organisms. Novel fluorescent tags were developed herein, based upon a genetically targeted fluorogen activating protein and cognate fluorogenic dye that yields emission with a peak at 733 nm exclusively when complexed as a “fluoromodule”. This tool improves substantially over previously described far-red/NIR fluorescent proteins in terms of brightness, wavelength, and flexibility by leveraging the flexibility of synthetic chemistry to produce novel chromophores.

    摘要翻译: 组织切片和整个生物体对荧光成像提供了重大挑战。 通过内在发色团(如黄素,黑色素和血红蛋白)的自发荧光和吸收会混淆和降解所有荧光标记的输出。 “光学窗口”比大多数自发荧光源更红,在低血红蛋白和吸水度的区域中位于650和900nm之间。 相对光学透明度的这个谷物是组织,完整器官和活生物体内基于荧光的研究的有吸引力的目标。 本文开发了基于遗传靶向荧光素激活蛋白和同源荧光染料的新型荧光标记,其在733nm处产生具有峰值的发射,当作为“荧光团”复合时。 通过利用合成化学的灵活性来生产新颖的发色团,该工具在亮度,波长和灵活性方面基本上优于之前描述的远红/ NIR荧光蛋白。

    Receptor-avid exogenous optical contrast and therapeutic agents
    5.
    发明授权
    Receptor-avid exogenous optical contrast and therapeutic agents 有权
    受体 - 狂热的外源性光学对比和治疗剂

    公开(公告)号:US07790144B2

    公开(公告)日:2010-09-07

    申请号:US10800531

    申请日:2004-03-15

    IPC分类号: A61B5/00 A61B10/00

    摘要: Cyanine dye bioconjugates useful for diagnostic imaging and therapy are disclosed. The conjugates include several cyanine dyes with a variety of bis- and tetrakis (carboxylic acid) homologes. The compounds are be conjugated to bioactive peptides, carbohydrates, hormones, drugs, or other bioactive agents. The small size of the compounds allows more favorable delivery to tumor cells as compared to larger molecular weight imaging agents. The various dyes are useful over the range of 350 to 1,300 nm, the exact range being dependent upon the particular dye. The use of a biocompatible organic solvent such as dimethylsulfoxide helps to maintain the fluorescence of the compounds. The inventive compounds are useful for diagnostic imaging and therapy, in endoscopic applications for the detection of tumors and other abnormalities, for localized therapy, for photoacoustic tumor imaging, detection and therapy, and for sonofluorescence tumor imaging, detection and therapy.

    摘要翻译: 公开了可用于诊断成像和治疗的花青染料生物缀合物。 缀合物包括几种具有多种双和四(羧酸)同系物的花青染料。 化合物与生物活性肽,碳水化合物,激素,药物或其他生物活性剂缀合。 与较大的分子量成像剂相比,化合物的小尺寸允许更有利于肿瘤细胞的递送。 各种染料在350至1,300nm的范围内是有用的,精确的范围取决于特定的染料。 使用生物相容性有机溶剂如二甲基亚砜有助于维持化合物的荧光。 本发明化合物可用于诊断成像和治疗,用于检测肿瘤和其他异常的内窥镜应用,局部治疗,光声肿瘤成像,检测和治疗以及用于声发射肿瘤成像,检测和治疗。

    Fluorescent detection method and reagent
    6.
    发明授权
    Fluorescent detection method and reagent 失效
    荧光检测方法和试剂

    公开(公告)号:US07662973B2

    公开(公告)日:2010-02-16

    申请号:US10182996

    申请日:2001-02-01

    IPC分类号: C09B7/02

    摘要: Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterized by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.

    摘要翻译: 公开了增加包含至少一个NO 2基团的花青染料分子的荧光的方法,其特征在于通过硝基还原酶的作用将至少一种NO 2基团还原成NHOH或NH 2。 包含至少一个NO 2基团的花青染料分子可用作组合物中硝基还原酶活性检测的底物,并且允许在酶报告系统中使用硝基还原酶用于检测分析物,结合反应或基因表达 。

    Laser thermal imaging process, dye, and element
    7.
    发明授权
    Laser thermal imaging process, dye, and element 失效
    激光热成像工艺,染料和元素

    公开(公告)号:US06703111B2

    公开(公告)日:2004-03-09

    申请号:US10032922

    申请日:2001-10-25

    IPC分类号: B32B2714

    摘要: A laser-induced thermal recording element comprising a support having thereon a colorant layer comprising a colorant dispersed in a polymeric binder, said colorant layer having associated therewith a laser light-absorbing dye absorbing at the wavelength of a laser used to expose said element, said laser light-absorbing dye comprising a polymethine (cyanine) dye having covalently bonded thereto a phenylenediamine moiety. The element exhibits improved dye stability.

    摘要翻译: 一种激光诱导的热敏记录元件,包括其上具有着色剂层的载体,所述着色剂层包含分散在聚合物粘合剂中的着色剂,所述着色剂层具有吸收在用于暴露所述元件的激光波长处吸收的激光吸收染料,所述激光吸收染料 激光吸收染料,其包含共价键合有苯二胺部分的聚甲基(花青)染料。 该元件显示改善的染料稳定性。

    Fluorescent detection method and reagent
    8.
    发明申请
    Fluorescent detection method and reagent 失效
    荧光检测方法和试剂

    公开(公告)号:US20030186348A1

    公开(公告)日:2003-10-02

    申请号:US10182996

    申请日:2002-10-16

    IPC分类号: C12Q001/26 C07D43/02

    摘要: Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterised by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reaction or gene expression.

    摘要翻译: 公开了增加包含至少一个NO 2基团的花青染料分子的荧光的方法,其特征在于通过硝基还原酶的作用将至少一种NO 2基团还原成NHOH或NH 2。 包含至少一个NO 2基团的花青染料分子可用作检测组合物中硝基还原酶活性的底物,并且允许在酶报告系统中使用硝基还原酶用于检测分析物,结合反应或基因表达 。

    Receptor-avid exogenous optical contrast and therapeutic agents
    9.
    发明申请
    Receptor-avid exogenous optical contrast and therapeutic agents 失效
    受体 - 狂热的外源性光学对比和治疗剂

    公开(公告)号:US20020156117A1

    公开(公告)日:2002-10-24

    申请号:US09864011

    申请日:2001-05-23

    申请人: MALLINCKRODT INC.

    IPC分类号: A61K031/405 C07D43/02

    摘要: Cyanine dye bioconjugates useful for diagnostic imaging and therapy are disclosed. The conjugates include several cyanine dyes with a variety of bis- and tetrakis (carboxylic acid) homologes. The compounds may be conjugated to bioactive peptides, carbohydrates, hormones, drugs, or other bioactive agents. The small size of the compounds allows more favorable delivery to tumor cells as compared to larger molecular weight imaging agents. The various dyes are useful over the range of 350 to 1,300 nm, the exact range being dependent upon the particular dye. The use of dimethylsulfoxide helps to maintain the fluorescence of the compounds. The inventive compounds are useful for diagnostic imaging and therapy, in endoscopic applications for the detection of tumors and other abnormalities, for localized therapy, for photoacoustic tumor imaging, detection and therapy, and for sonofluorescence tumor imaging, detection and therapy.

    摘要翻译: 公开了可用于诊断成像和治疗的花青染料生物缀合物。 缀合物包括几种具有多种双和四(羧酸)同系物的花青染料。 化合物可以与生物活性肽,碳水化合物,激素,药物或其他生物活性剂缀合。 与较大的分子量成像剂相比,化合物的小尺寸允许更有利于肿瘤细胞的递送。 各种染料在350至1,300nm的范围内是有用的,精确的范围取决于特定的染料。 使用二甲基亚砜有助于维持化合物的荧光。 本发明化合物可用于诊断成像和治疗,用于检测肿瘤和其他异常的内窥镜应用,局部治疗,光声肿瘤成像,检测和治疗以及用于声发射肿瘤成像,检测和治疗。

    Dendrimer precursor dyes for imaging
    10.
    发明授权
    Dendrimer precursor dyes for imaging 有权
    用于成像的树枝状染料前体染料

    公开(公告)号:US06395257B1

    公开(公告)日:2002-05-28

    申请号:US09484322

    申请日:2000-01-18

    IPC分类号: A61B500

    摘要: The sensitivity and specificity of the optical modality can be enhanced by the use of highly absorbing dyes as contrast agents. Novel cyanine dyes that absorb and emit light in the near infrared region of electromagnetic spectrum are disclosed. These dyes are useful for imaging, diagnosis and therapy of various diseased states. Particularly, the molecules of the invention are useful for optical diagnostic imaging and therapy, in endoscopic applications for the detection of tumors and other abnormalities, for localized therapy, for photoacoustic tumor imaging, detection and therapy, and for sonofluorescence tumor imaging, detection and therapy.

    摘要翻译: 通过使用高吸收性染料作为造影剂可以提高光学模态的灵敏度和特异性。 公开了在电磁光谱的近红外区域吸收和发光的新型花青染料。 这些染料可用于各种疾病状态的成像,诊断和治疗。 特别地,本发明的分子可用于光学诊断成像和治疗,用于检测肿瘤和其他异常的内窥镜应用,局部治疗,光声肿瘤成像,检测和治疗以及用于声发射肿瘤成像,检测和治疗 。