公开(公告)号：US06273262B1

公开(公告)日：2001-08-14

申请号：US09317608

申请日：1999-05-25

Applicant: Kenji Yasuda ,  Takeshi Sakamoto

Inventor： Kenji Yasuda ,  Takeshi Sakamoto

IPC: B03B566

CPC classification number: B01D21/283 ,  G01N1/40 ,  G01N29/223 ,  G01N2291/02416

Abstract: A device comprises a means for radiating ultrasound from one face of a chamber holding a sample solution containing particles to be concentrated, and a reflective face inclined to the face irradiated with the ultrasound. The frequency of the ultrasound radiated from the ultrasound radiating means is changed periodically and asymmetrically with the passage of time.

Abstract translation: 一种装置包括用于从保持含有待浓缩颗粒的样品溶液的室的一个面的一个面辐射超声波的装置和与被超声波照射的面倾斜的反射面。 从超声波辐射装置发射的超声波的频率随着时间的推移而周期性地和不对称地改变。

公开(公告)号：US06251659B1

公开(公告)日：2001-06-26

申请号：US09419918

申请日：1999-10-18

Applicant: Shinichi Fukuzuno ,  Norie Maeuchihara ,  Toshinari Sakurai ,  Kenji Yasuda

Inventor： Shinichi Fukuzuno ,  Norie Maeuchihara ,  Toshinari Sakurai ,  Kenji Yasuda

IPC: C12M136

CPC classification number: C12M41/12 ,  B01L3/50851 ,  B01L7/00 ,  B01L7/5255 ,  B01L2300/1805 ,  G01N2035/00396

Abstract: A rectangular-shaped holder containing sample solution is moved within a temperature adjusting box by a feeder. Then, the holder is moved by another feeder to the direction substantially perpendicular to the longitudinal direction of the holder by a length equal to the width of the single holder. The holders thus sequentially fed within the temperature adjusting box are disposed within the box in a closely contacted state from one another. There are three holders within the temperature adjusting box in advance and four holders are stayed within the box. Rectangular-shaped four heaters are disposed within the temperature adjusting box such that the heating temperature thereof is controlled by a temperature adjusting controller. A compensation heater controlled by a temperature compensating controller compensates for a heat quantity shifted to the holder having been newly housed within the temperature adjusting box from the holders already housed within the temperature adjusting box.

Abstract translation: 含有样品溶液的矩形保持架通过进料器在温度调节箱内移动。 然后，保持器通过另一馈送器移动到与保持器的纵向方向基本垂直的方向等于单个保持器的宽度的长度。 因此，在温度调节箱内依次进给的保持器以彼此紧密接触的状态设置在箱体内。 提前温度调节箱内有三个支架，四个支架都保持在箱内。 矩形四个加热器设置在温度调节箱内，使其加热温度由温度调节控制器控制。 由温度补偿控制器控制的补偿加热器从已经容纳在温度调节箱内的保持器补偿从温度调节箱中新装入的保持器的热量。

公开(公告)号：US06183970B2

公开(公告)日：2001-02-06

申请号：US09383198

申请日：1999-08-26

Applicant: Kazunori Okano ,  Hideki Kambara ,  Chihiro Uematsu ,  Hiroko Matsunaga ,  Takashi Irie ,  Tomoharu Kajiyama ,  Kenji Yasuda

Inventor： Kazunori Okano ,  Hideki Kambara ,  Chihiro Uematsu ,  Hiroko Matsunaga ,  Takashi Irie ,  Tomoharu Kajiyama ,  Kenji Yasuda

IPC: C12Q168

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0819 ,  B01L2400/0421 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/607 ,  C12Q2561/119

Abstract: There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.

Abstract translation: 预先制备具有反应残基的单体和含有残基与反应残基连接的多种多核苷酸探针的多核苷酸探针组。 将单体与包含选自多核苷酸探针组的任何多个探针的各种多核苷酸探针混合。 将各种所得混合物加入每个不同的小孔中以使混合物成为凝胶基质。 因此，产生多核苷酸探针芯片。 样品DNA通过电泳在凝胶中强制迁移。 激光投射到芯片的侧面。 从芯片的整个表面发射的荧光由高灵敏度二维检测器共同检测。 因此，可以提供用于检测DNA的保持各种DNA探针的多核苷酸探针芯片。 该芯片具有高杂交效率，可实现高灵敏度和高速DNA检测。

公开(公告)号：US6093370A

公开(公告)日：2000-07-25

申请号：US329318

申请日：1999-06-10

Applicant: Kenji Yasuda ,  Kazunori Okano ,  Hirokazu Kato

Inventor： Kenji Yasuda ,  Kazunori Okano ,  Hirokazu Kato

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  C40B40/06 ,  G01N33/00

CPC classification number: B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6806 ,  C12Q1/6837 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0825 ,  B01L2300/0838 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0421 ,  B01L2400/0454 ,  B01L2400/0487 ,  B01L3/50851 ,  B01L7/54 ,  C40B40/06

Abstract: Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

公开(公告)号：US5707875A

公开(公告)日：1998-01-13

申请号：US513510

申请日：1995-08-10

Applicant: Mitsuru Tamura ,  Yoshinori Harada ,  Norio Shimizu ,  Kenji Yasuda

Inventor： Mitsuru Tamura ,  Yoshinori Harada ,  Norio Shimizu ,  Kenji Yasuda

IPC: G01R33/46 ,  G01N24/08 ,  G01N33/00 ,  G01V3/00

CPC classification number: G01R33/4608 ,  Y10T436/13 ,  Y10T436/16 ,  Y10T436/166666 ,  Y10T436/24

Abstract: Using a mixed sample of a plurality of phosphoric acid compounds different in chemical structure as a sample for measurement, a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P is observed and signals obtained without polarization transfer are eliminated, whereby .sup.31 P nuclear magnetic resonance signal spectrum of an .sup.17 O-labeled phosphoric acid is selectively observed. For this purpose, a double resonance NMR detector capable of exciting .sup.17 O and .sup.31 P at the same time is connected to an NMR spectrometer, and using a pulse train which permits observation of a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P, a .sup.31 P nuclear magnetic resonance signal from the .sup.17 O-labeled phosphoric acid is observed, and signals for .sup.17 O-unlabeled .sup.31 P are eliminated.

Abstract translation: 使用化学结构不同的多种磷酸化合物的混合样品作为测量样品，观察到通过17O和31P之间的极化转移获得的31P核磁共振信号，消除了没有极化转移的信号，由此31P核磁 选择性地观察到17O标记的磷酸的共振信号光谱。 为此，能够同时激发17O和31P的双共振NMR检测器连接到NMR光谱仪，并且使用允许观察通过17O和31P之间的极化转移获得的31P核磁共振信号的脉冲串， 观察到来自17O标记的磷酸的31P核磁共振信号，并且消除了17O-未标记的31P的信号。

公开(公告)号：US5523842A

公开(公告)日：1996-06-04

申请号：US483600

申请日：1995-06-07

Applicant: Kenji Yasuda ,  Masami Yoneda

Inventor： Kenji Yasuda ,  Masami Yoneda

IPC: G01B9/02 ,  G01B11/24 ,  G01B11/30

CPC classification number: G01B11/2441

Abstract: A method for interference fringe analysis in which a subject surface is located in parallel to a reference surface and multiply step shifted a specific distance for each step to derive data and images of interference fringes for determining aspects of the geometry of the subject surface.

Abstract translation: 一种用于干涉条纹分析的方法，其中对象表面位于与参考表面平行的位置，并且对每个步骤进行乘法步长偏移特定距离以导出干涉条纹的数据和图像，以确定对象表面的几何形状。

公开(公告)号：US5523058A

公开(公告)日：1996-06-04

申请号：US240733

申请日：1994-05-12

Applicant: Shinichiro Umemura ,  Kenichi Kawabata ,  Kenko Uchida ,  Kenji Yasuda ,  Yasuo Wada ,  Atsushi Hiraiwa

Inventor： Shinichiro Umemura ,  Kenichi Kawabata ,  Kenko Uchida ,  Kenji Yasuda ,  Yasuo Wada ,  Atsushi Hiraiwa

IPC: A61B17/22 ,  A61N7/00 ,  A61N7/02 ,  B01F11/02 ,  A61B8/00 ,  B06B1/00

CPC classification number: A61N7/02 ,  A61B17/22004 ,  A61N2007/006 ,  A61N2007/0078 ,  A61N2007/0095

Abstract: By providing an ultrasonic irradiation apparatus for generating acoustic cavitation efficiently, it is intended to realize an ultrasonic therapeutic apparatus for generating the action of cavitation on a living body suitable for medical treatment of malignant tumors and medical treatment of thrombi and calculi, an ultrasonic diagnostic apparatus for generating cavitation for emphasizing an ultrasonic echo image such as a blood flow and utilizing the reflection capability of the cavitation, an ultrasonic chemical reaction accelerating apparatus, an ultrasonic cleaning apparatus or an ultrasonic sterilizing apparatus. Irradiation focus/code signals for defining irradiated acoustic fields of a fundamental wave and a second harmonic wave as well as focus positions/acoustic pressure distribution forms of the respective waves are applied from an irradiation unit main control circuit to drive phase generating circuits. Generated drive phases are applied to drive signal generating circuits, generated drive signals are applied to element drive circuits and a group of fundamental frequency elements and a group of second harmonic elements are driven. The drive phases are controlled such that the fundamental wave and second harmonic wave are superimposed on each other in a medium near a focal point, thus generating acoustic caviation locally and efficiently.

Abstract translation: PCT No.PCT / JP93 / 01310 Sec。 371日期：1994年5月12日 102（e）日期1994年5月12日PCT提交1993年9月14日PCT公布。 公开号WO94 / 06380 日期为1994年3月31日。通过提供用于有效产生声孔的超声波照射装置，旨在实现用于产生适于恶性肿瘤治疗的生物体上的空化作用的超声波治疗装置和血栓的治疗 和结石，用于产生用于强调超声波回波图像如血流并利用空化反射能力的超声波诊断装置，超声波化学反应加速装置，超声波清洗装置或超声波消毒装置。 用于定义基波和二次谐波的辐射声场以及各波的聚焦位置/声压分布形式的照射焦点/码信号从照射单元主控制电路施加到驱动相位产生电路。 产生的驱动相位被施加到驱动信号发生电路，所产生的驱动信号被施加到元件驱动电路，并且一组基频元件和一组二次谐波元件被驱动。 控制驱动相位使得基波和二次谐波在焦点附近的介质中彼此叠加，从而局部且有效地产生声空化。

公开(公告)号：US5407568A

公开(公告)日：1995-04-18

申请号：US118704

申请日：1993-09-09

Applicant: Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

Inventor： Junkichi Miura ,  Kenji Yasuda ,  Mitsuo Ito ,  Masahito Ito

IPC: G01N30/02 ,  B01D15/08 ,  B01J39/26 ,  G01N30/50 ,  G01N30/96

CPC classification number: G01N30/96 ,  B01D15/20 ,  B01D15/363 ,  B01J39/26

Abstract: Deterioration of performance of a separation column for liquid chromatograph due to changes in the functional groups during the preservation or application is prevented by filling a solution containing a component in the molecule with the same structure as that of the chemical function group of a packing material for the separation column as a filling liquid together with the packing material in the separation column, and also by washing the separation column with the same filling liquid as a washing solution after the analysis and retaining the washing solution therein, whereby the preservation stability of the separation column is improved and the separation column can be preserved at room temperature.

Abstract translation: 通过将包含分子中的成分的溶液与包装材料的化学官能团相同的结构填充来防止由于保存或施加期间官能团的变化而导致的液相色谱分离柱的性能劣化 分离塔作为填充液与分离塔中的包装材料一起，并且在分析后保持与洗涤液相同的填充液，同时通过洗涤分离塔，从而保持分离的保存稳定性 柱被改善，分离柱可以在室温下保存。

公开(公告)号：US5358639A

公开(公告)日：1994-10-25

申请号：US38396

申请日：1993-03-29

Applicant: Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

Inventor： Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

IPC: G01N30/26 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N30/50 ,  G01N30/52 ,  G01N30/88 ,  G01N33/72 ,  B01D15/08

CPC classification number: G01N30/52 ,  G01N2030/525 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N33/72

Abstract: In the analysis of hemoglobins in blood samples by chromatography, an increase in the pressure in a flow passage line is suppressed, a separation column is made durable against prolonged use by supplying phosphate-based buffer solutions as eluting solutions to a separation column to a separation column and a solution containing not more than 100 mM of S-(carboxyalkyl)-L-cysteine and phosphate-based buffer agent thereto in the course of a series of analyzing steps.

Abstract translation: 在通过色谱法对血液样品中的血红蛋白进行分析时，抑制流路线中的压力增加，通过将磷酸盐类缓冲溶液作为分离柱的洗脱溶液供给至分离液，使分离塔长期使用耐久性 柱和在一系列分析步骤中含有不超过100mM的S-（羧基烷基）-L-半胱氨酸和磷酸盐缓冲剂的溶液。

公开(公告)号：US5252533A

公开(公告)日：1993-10-12

申请号：US856796

申请日：1992-03-24

Applicant: Kenji Yasuda ,  Toshihiro Minato ,  Masaru Kato ,  Toshikazu Nagura ,  Hiroshi Arakawa

Inventor： Kenji Yasuda ,  Toshihiro Minato ,  Masaru Kato ,  Toshikazu Nagura ,  Hiroshi Arakawa

IPC: B41M5/41 ,  B41M5/035 ,  B41M5/38

CPC classification number: B41M5/41 ,  Y10S428/913 ,  Y10S428/914 ,  Y10T428/24942 ,  Y10T428/2495 ,  Y10T428/24967 ,  Y10T428/24975 ,  Y10T428/24992 ,  Y10T428/31511 ,  Y10T428/31786 ,  Y10T428/31797 ,  Y10T428/31855

Abstract: A thermal transfer dye image-receiving sheet capable of receiving clear, uniform colored images without a formation of curls and wrinkles therein, comprising (A) a substrate sheet composed of (a) core sheet having a Young's modulus E.sub.3, (b) a front coated thermoplastic film layer having a thickness T.sub.1 and a Young's modulus E.sub.1, and (c) a back coated thermoplastic film layer having a thickness T.sub.2 and a Young's modulus E.sub.2, the T.sub.1, T.sub.2, E.sub.1, E.sub.2 and E.sub.3 satisfying the following relationships (1) and (2):T.sub.2 .gtoreq.T.sub.1 (1)andE.sub.3 .gtoreq.2E.sub.1 .gtoreq.2E.sub.2 (2)wherein Y.sub.1, Y.sub.2 and Y.sub.3 are determined in accordance with ASTM D882-64T.

Abstract translation: 一种热转印染料影像接收片材，其能够接收清晰均匀的彩色图像而不形成卷曲和皱纹，其包括（A）由（a）具有杨氏模量E3的芯片组成的基片，（b） 具有厚度T1和杨氏模量E1的涂布热塑性薄膜层，（c）具有厚度T2和杨氏模量E2，T1，T2，E1，E2和E3的背涂热塑性薄膜层，满足以下关系式（1 ）和（2）：T2> / = T1（1）和E3> / = 2E1> / = 2E2（2）其中Y1，Y2和Y3根据ASTM D882-64T测定。