公开(公告)号：US20140113324A1

公开(公告)日：2014-04-24

申请号：US14057942

申请日：2013-10-18

Applicant: The Regents Of The University Of California

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse ,  Aram Chung

IPC: G01N33/50

CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N2021/6439 ,  G06K9/4604 ,  G06T7/0004 ,  G06T7/0016

Abstract: A system for deforming and analyzing particles includes a substrate defining an inlet, and an outlet; a fluidic pathway fluidly coupled to the inlet and the outlet and defining a delivery region upstream of a deformation region configured to deform particles, wherein the fluidic pathway comprises a first branch configured to generate a first flow, and a second branch configured to generate a second flow that opposes the first flow, wherein an intersection of the first flow and the second flow defines the deformation region; a detection module including a sensor configured to generate a morphology dataset characterizing deformation of the particles, and a photodetector configured to generate a fluorescence dataset characterizing fluorescence of the particles; and a processor configured to output an analysis of the plurality of particles based at least in part on the deformation dataset and the fluorescent dataset for the plurality of particles.

Abstract translation: 用于变形和分析颗粒的系统包括限定入口的基板和出口; 流体路径，流体地联接到入口和出口，并且限定被配置为变形颗粒的变形区域上游的输送区域，其中流体路径包括被配置为产生第一流量的第一分支和被配置为产生第二流量的第二分支 所述第一流与所述第一流相对，其中所述第一流和所述第二流的交点限定所述变形区; 检测模块，其包括被配置为生成表征所述粒子的变形的形态学数据集的传感器，以及被配置为产生表征所述粒子的荧光的荧光数据集的光电检测器; 以及处理器，被配置为至少部分地基于所述多个粒子的所述变形数据集和所述荧光数据集来输出所述多个粒子的分析。

公开(公告)号：US08293524B2

公开(公告)日：2012-10-23

申请号：US11690831

申请日：2007-03-25

Applicant: Cristian Ionescu-Zanetti ,  Michelle Khine ,  Michael Schwartz ,  Andrew Blatz

Inventor： Cristian Ionescu-Zanetti ,  Michelle Khine ,  Michael Schwartz ,  Andrew Blatz

IPC: C12M1/34 ,  C12M3/00

CPC classification number: G01N15/1404 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/565 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/163 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M21/06 ,  C12M23/16 ,  C12M35/04 ,  C12M41/36 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1475 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1409 ,  G01N2015/1495 ,  G01N2015/1497

Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.

Abstract translation: 提供了用于分析微流体装置中的各个颗粒的装置和方法。 这些方法涉及在悬浮液中固定一系列颗粒并应用实验化合物。 这样的方法还可以包括电生理学研究，包括在同一微流体装置中的膜片钳记录，电穿孔或两者。 提供的装置包括耦合到多孔结构的微流体装置和用于控制微通道装置内的介质流动的界面。

公开(公告)号：US20040166025A1

公开(公告)日：2004-08-26

申请号：US10775599

申请日：2004-02-10

Applicant: U.S. Genomics, Inc.

Inventor： Eugene Y. Chan ,  Lance C. Gleich ,  Parris S. Wellman

IPC: B01L003/00

CPC classification number: G01N15/1456 ,  B01F5/061 ,  B01F5/064 ,  B01F5/0646 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N1/286 ,  G01N15/1459 ,  G01N33/442 ,  G01N2015/1493 ,  G01N2015/1495 ,  Y10T436/143333

Abstract: The present invention provides structures and methods that allow polymers of any length, including nucleic acids containing entire genomes, to be stretched into a long, linear conformation for further analysis. The present invention also provides structures and methods for selecting and stretching polymers based on their lengths. Polymers are loaded into a device and run through the structures. Stretching is achieved by, e.g., applying shear forces as the polymer passes through the structures, placing obstacles in the path of the polymer, or a combination thereof. Since multiple molecules may be stretched in succession, extremely high throughput screening, e.g., screening of more than one molecule per second, is achieved.

公开(公告)号：US20020039737A1

公开(公告)日：2002-04-04

申请号：US09875779

申请日：2001-06-06

Inventor： Eugene Y. Chan ,  Lance C. Gleich ,  Parris S. Wellman

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  G01N033/558

CPC classification number: B01L3/502761 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0816 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N1/286 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1463 ,  G01N33/442 ,  G01N2015/1075 ,  G01N2015/1493 ,  G01N2015/1495 ,  Y10T436/13 ,  Y10T436/143333

Abstract: A method for determining the length and velocity of single elongated macromolecules is disclosed. In particular, the present invention relates to methods and apparatus for determining the velocity of elongated polymeric molecules moving relative to one or more detection stations, as well as to methods and apparatus for determining the length of such molecules and the distance between landmarks that may be present on such molecules. The invention makes use of time correlated measurements of signal amplitude profiles that result from interactions between each detection station and portions of each macromolecule.

Abstract translation: 公开了一种用于确定单个细长大分子的长度和速度的方法。 特别地，本发明涉及用于确定相对于一个或多个检测站移动的细长聚合物分子的速度的方法和装置，以及用于确定这种分子长度的方法和装置以及标记之间的距离 存在于这样的分子上。 本发明利用由每个检测站与每个大分子的部分之间的相互作用产生的信号幅度分布的时间相关测量。

公开(公告)号：US12007321B2

公开(公告)日：2024-06-11

申请号：US17200728

申请日：2021-03-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse ,  Aram Chung

IPC: G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G01N21/64 ,  G01N33/50 ,  G06T7/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/147 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G06T7/0004 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N21/64 ,  G01N2021/6439 ,  G06T7/0016

Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.

公开(公告)号：US20240102915A1

公开(公告)日：2024-03-28

申请号：US17766562

申请日：2020-10-06

Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFQUE (CNRS) ,  JUNIA

Inventor： Dominique COLLARD ,  Hiroyuki FUJITA ,  Stanislav KARSTEN ,  Mehmet Cagatay TARHAN

IPC: G01N15/14 ,  B01L3/00 ,  G01N15/10 ,  G01N33/487

CPC classification number: G01N15/1484 ,  B01L3/502761 ,  G01N15/1031 ,  G01N33/48728 ,  B01L2200/0663 ,  B01L2300/0645 ,  G01N2015/1006 ,  G01N2015/1495

Abstract: The invention relates to a measuring device (10) for measuring physical characteristics of cells. The device (10) comprises: a microfluidic chip (20) provided with a flow channel (22) for allowing cells to flow through; a manipulator (24) configured to apply deformation force to a cell in a continuous flow; and a sensor (26) configured to sense a physical characteristic of the cell. The manipulator (24) and the sensor (26) are configured to define a width (W2) of the flow channel (22) as a gap formed between them. The manipulator (24) is configured to apply the deformation force to the cell by compressing the cell against the sensor (26).

公开(公告)号：US11860080B2

公开(公告)日：2024-01-02

申请号：US17564489

申请日：2021-12-29

Applicant: Rolls-Royce Corporation ,  Rolls-Royce PLC

Inventor： Scott Nelson ,  Taylor K. Blair ,  John Grubbs ,  Martyn Anthony Jones ,  Chris Heason

IPC: G01N15/14

CPC classification number: G01N15/1463 ,  G01N15/1459 ,  G01N2015/1495 ,  G01N2015/1497

Abstract: In some examples, a system including a fluid stream monitoring system. The monitoring system includes an illumination device configured to illuminate at least some of particles suspended in a fluid stream; and an imaging device configured to image the illuminated particles at a first image plane that intersects a longitudinal axis of the fluid stream, wherein the illumination device and the imaging device are registered to the fluid stream delivery device in the first image plane, where the first image plane is substantially orthogonal to the longitudinal axis. The system includes processing circuitry configured to determine one or more physical characteristics of the fluid particles.

公开(公告)号：US11788950B2

公开(公告)日：2023-10-17

申请号：US16479689

申请日：2018-01-26

Applicant: UNIVERSITÄT ULM

Inventor： Daniel Geiger ,  Tobias Neckernuss ,  Othmar Marti

IPC: G01N15/14 ,  G06T7/00 ,  G06T7/269 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N15/1475 ,  G01N1/40 ,  G01N15/1431 ,  G01N15/1436 ,  G06T7/0016 ,  G06T7/269 ,  G01N2015/0019 ,  G01N2015/1081 ,  G01N2015/1495 ,  G06T2207/30024

Abstract: Disclosed is a method for analysing cells, in which cells are separated and the individual cells pass via a measurement region of a unit for spatially resolved radiation intensity measurement, wherein, for at least one of the separated cells, when passing via the measurement region, a time sequence of spatial intensity patterns of an electromagnetic radiation emitted from and/or influenced by the cell is created, the optical flow of a respective two of the spatial intensity patterns is calculated for at least one portion of the sequence of intensity patterns using a computer unit, and an evaluation of the calculated optical flows occurs. Also disclosed is a device for analysing cells, comprising a device for separating cells, a unit for spatially resolved radiation intensity measurement, and a computer unit for calculating the optical flow of a respective two of the created intensity patterns, and for evaluating the calculated optical flows.

公开(公告)号：US20180128735A1

公开(公告)日：2018-05-10

申请号：US15868025

申请日：2018-01-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse

IPC: G01N15/14 ,  G06F19/10 ,  B01L3/00 ,  C12Q1/02 ,  G01N33/487 ,  G01N33/574 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G16B99/00

Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

公开(公告)号：US20170234788A1

公开(公告)日：2017-08-17

申请号：US15471851

申请日：2017-03-28

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T.K. Tse ,  Aram Chung

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/147 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N2021/6439 ,  G06K9/4604 ,  G06T7/0004 ,  G06T7/0016

Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.