THERMOSTABLE CELLOBIOHYDROLASE
    21.
    发明申请
    THERMOSTABLE CELLOBIOHYDROLASE 有权
    可热释放的碳水化合物

    公开(公告)号:US20160168549A1

    公开(公告)日:2016-06-16

    申请号:US14964980

    申请日:2015-12-10

    摘要: A thermostable cellobiohydrolase, having a cellobiohydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5, or (C) a polypeptide including an amino acid sequence having 60% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.

    摘要翻译: 一种具有纤维二糖水解酶催化结构域的热稳定纤维二糖水解酶,包括:(A)包含SEQ ID NO:1或2所示氨基酸序列的多肽,(B)包含至少一个氨基酸的氨基酸序列的多肽 或者(C)至少在75℃和pH5的条件下具有对磷酸溶胀的Avicel底物具有水解活性的氨基酸序列, 多肽,其包含与SEQ ID NO:1或2所示的氨基酸序列具有60%以上序列同一性的氨基酸序列,并且至少在75℃的条件下对磷酸溶胀的Avicel的底物具有水解活性。 和pH 5。

    Variants of glycoside hydrolases
    23.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号:US08383385B2

    公开(公告)日:2013-02-26

    申请号:US13009524

    申请日:2011-01-19

    摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译: 本发明涉及母体糖苷水解酶的变体,其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代,和 486,SEQ ID NO:2的氨基酸1至513,并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代, SEQ ID NO:2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113, SEQ ID NO:2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462,其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体,载体和宿主细胞。

    Variants of glycoside hydrolases
    27.
    发明授权

    公开(公告)号:US09701951B2

    公开(公告)日:2017-07-11

    申请号:US14475639

    申请日:2014-09-03

    申请人: Novozymes Inc.

    摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    Variants of glycoside hydrolases
    30.
    发明申请
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号:US20080167214A1

    公开(公告)日:2008-07-10

    申请号:US11891249

    申请日:2007-08-08

    摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译: 本发明涉及母体糖苷水解酶的变体,其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代,和 486,SEQ ID NO:2的氨基酸1至513,并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代, SEQ ID NO:2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113, SEQ ID NO:2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462,其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体,载体和宿主细胞。