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公开(公告)号:US20160168549A1
公开(公告)日:2016-06-16
申请号:US14964980
申请日:2015-12-10
发明人: Jiro OKUMA , Migiwa SUDA , Asuka YAMAGUCHI , Yoshitsugu HIROSE , Yasuhiro KONDO , Masaru SATO , Daisuke SHIBATA
CPC分类号: C12N9/2434 , C12N9/2437 , C12N9/2482 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01008 , C12Y302/01176
摘要: A thermostable cellobiohydrolase, having a cellobiohydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5, or (C) a polypeptide including an amino acid sequence having 60% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.
摘要翻译: 一种具有纤维二糖水解酶催化结构域的热稳定纤维二糖水解酶,包括:(A)包含SEQ ID NO:1或2所示氨基酸序列的多肽,(B)包含至少一个氨基酸的氨基酸序列的多肽 或者(C)至少在75℃和pH5的条件下具有对磷酸溶胀的Avicel底物具有水解活性的氨基酸序列, 多肽,其包含与SEQ ID NO:1或2所示的氨基酸序列具有60%以上序列同一性的氨基酸序列,并且至少在75℃的条件下对磷酸溶胀的Avicel的底物具有水解活性。 和pH 5。
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公开(公告)号:US20150291947A1
公开(公告)日:2015-10-15
申请号:US14437760
申请日:2013-10-22
申请人: NOVOZYMES A/S
发明人: Peter WESTH , Jeppe KARI , Johan OLSEN , Kim BORCH , Kenneth JENSEN , Kristian B. R. M. KROGH
IPC分类号: C12N9/42 , C12P19/12 , C12P7/02 , C12P7/18 , C12P7/16 , C12P7/10 , C12P7/20 , C12P7/04 , C12P5/02 , C12P5/00 , C12P13/20 , C12P13/14 , C12P13/00 , C12P13/08 , C12P13/06 , C12P3/00 , C12P7/26 , C12P7/28 , C12P7/40 , C12P7/54 , C12P7/44 , C12P17/04 , C12P7/48 , C12P7/58 , C12P7/42 , C12P7/56 , C12P7/52 , C12P7/46 , C12P19/14
CPC分类号: C12N9/2437 , C12P3/00 , C12P5/002 , C12P5/007 , C12P5/02 , C12P5/023 , C12P5/026 , C12P7/02 , C12P7/04 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P7/42 , C12P7/44 , C12P7/46 , C12P7/48 , C12P7/52 , C12P7/54 , C12P7/56 , C12P7/58 , C12P13/005 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/20 , C12P17/04 , C12P19/12 , C12P19/14 , C12Y302/01091 , C12Y302/01176 , Y02E50/16
摘要: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译: 本发明涉及纤维二糖水解酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。
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公开(公告)号:US08383385B2
公开(公告)日:2013-02-26
申请号:US13009524
申请日:2011-01-19
申请人: Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
发明人: Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
CPC分类号: C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译: 本发明涉及母体糖苷水解酶的变体,其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代,和 486,SEQ ID NO:2的氨基酸1至513,并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代, SEQ ID NO:2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113, SEQ ID NO:2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462,其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体,载体和宿主细胞。
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公开(公告)号:US10081824B2
公开(公告)日:2018-09-25
申请号:US15265460
申请日:2016-09-14
申请人: Novozymes, Inc.
CPC分类号: C12P19/14 , C07K14/38 , C12N9/2437 , C12N9/2445 , C12P19/02 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , C12Y302/01176 , Y02E50/16 , Y02P20/52
摘要: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
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25.
公开(公告)号:US20170283812A1
公开(公告)日:2017-10-05
申请号:US15478871
申请日:2017-04-04
CPC分类号: C12N15/76 , C12N9/2434 , C12N9/2437 , C12N15/52 , C12P19/02 , C12P19/14 , C12P2203/00 , C12Y302/01004 , C12Y302/01091 , C12Y302/01176
摘要: A heterologous gene cassette useful for creating Streptomyces species with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.
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公开(公告)号:US20170204441A1
公开(公告)日:2017-07-20
申请号:US15316477
申请日:2015-06-04
申请人: Novozymes A/S
发明人: Jeffrey Shasky , Debbie Yaver , Donna Moyer
CPC分类号: C12P19/14 , C12N9/0004 , C12N9/0065 , C12N9/24 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/02 , C12P2201/00 , C12P2203/00 , C12Y111/01006 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , C12Y302/01176 , Y02E50/16
摘要: The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.
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公开(公告)号:US09701951B2
公开(公告)日:2017-07-11
申请号:US14475639
申请日:2014-09-03
申请人: Novozymes Inc.
发明人: Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
CPC分类号: C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
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28.
公开(公告)号:US20150004655A1
公开(公告)日:2015-01-01
申请号:US14367182
申请日:2012-12-20
申请人: Novozymes, Inc.
发明人: Mark Wogulis
CPC分类号: C12P19/14 , C12N9/2437 , C12P7/14 , C12P19/02 , C12P2203/00 , C12Y302/01091 , C12Y302/01176 , G01N31/226
摘要: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.
摘要翻译: 本发明涉及纤维二糖水解酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 以及生产和使用这些变体的方法。
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公开(公告)号:US20110136167A1
公开(公告)日:2011-06-09
申请号:US13009524
申请日:2011-01-19
申请人: Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
发明人: Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
CPC分类号: C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
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公开(公告)号:US20080167214A1
公开(公告)日:2008-07-10
申请号:US11891249
申请日:2007-08-08
申请人: Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
发明人: Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
CPC分类号: C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译: 本发明涉及母体糖苷水解酶的变体,其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代,和 486,SEQ ID NO:2的氨基酸1至513,并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代, SEQ ID NO:2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113, SEQ ID NO:2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462,其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体,载体和宿主细胞。
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