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公开(公告)号：US11597952B2
公开(公告)日：2023-03-07
申请号：US16624868
申请日：2017-06-23
摘要： The aim of the invention is to provide a method for biotechnological production of carboxylic acids, in which the acid-forming micro-organisms are cultured in an unsterile manner in a submerged phase containing waste water containing all carbon and nutrient medium components necessary for the production of the carboxylic acid, which method avoids the disadvantages of known methods and enables high product concentrations and productivity while at the same time the resources of water and power are being conserved. This aim is achieved, according to the invention, in that micro-organisms are used that are cultured under unsterile conditions in a culture medium containing waste water with the addition of carbon-rich compounds.
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公开(公告)号：US11434508B2
公开(公告)日：2022-09-06
申请号：US16856143
申请日：2020-04-23
申请人： DSM IP ASSETS B.V.
IPC分类号： C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P17/10 , C12P19/02 , C12P19/14 , C12P35/00 , C12P5/02
摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
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公开(公告)号：US11434467B2
公开(公告)日：2022-09-06
申请号：US16467990
申请日：2018-12-24
发明人： Guiyang Shi , Zhijie Hu , Youran Li , Xiaodong Jiang , Sai Jin , Fuxin Sun , Cheng Zhang , Dongjiao Zhou , Jiawei Lu , Maodong Miao , Zihao Fan
摘要： Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.
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4.发明申请公开(公告)号：US20200248209A1
公开(公告)日：2020-08-06
申请号：US16856143
申请日：2020-04-23
申请人： DSM IP ASSETS B.V.
IPC分类号： C12P7/10 , C12P35/00 , C12P17/10 , C12P7/56 , C12P7/54 , C12P7/48 , C12P7/46 , C12P7/42 , C12P7/40 , C12P7/20 , C12P7/18 , C12P7/16 , C12P5/02 , C12P19/02 , C12P19/14
摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
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公开(公告)号：US10717995B2
公开(公告)日：2020-07-21
申请号：US16152197
申请日：2018-10-04
申请人： DSM IP ASSETS B.V.
IPC分类号： C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P17/10 , C12P19/02 , C12P19/14 , C12P35/00 , C12P5/02
摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
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公开(公告)号：US20190038595A1
公开(公告)日：2019-02-07
申请号：US16144246
申请日：2018-09-27
申请人： Diana Naturals
发明人： Philippe Sanoner , Valerie Bochard , Lucie Charissou , Benedicte Lastique , Morgane Jacob , Patrice Thomas
IPC分类号： A61K31/353 , A61K31/192 , A61K31/194 , A61K31/191 , A61K36/45 , A23L33/105 , C12P17/06 , C12P7/40 , C12P7/48 , C12P7/42 , C12P7/46
摘要： The present invention relates to a concentrated extract of cranberry (Vaccinium macrocarpon), the complex composition of which makes it possible to increase the antibacterial effects thereof, which is of use for the prevention or treatment of urinary infections, and in particular for the preventive treatment of urinary infections or treatment against the recurrence thereof. The invention also relates to a process for preparing such an extract, to food, nutraceutical or pharmaceutical compositions comprising the extract and to the use thereof in the treatment or prevention of urinary infections.
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公开(公告)号：US20180371447A1
公开(公告)日：2018-12-27
申请号：US16121617
申请日：2018-09-04
申请人： DSM IP ASSETS B.V.
IPC分类号： C12N9/90 , C12P35/00 , C12P13/04 , C12P7/56 , C12P7/54 , C12P7/52 , C12P7/48 , C12P7/46 , C12P7/42 , C12P7/40 , C12P7/20 , C12P7/18 , C12P7/16 , C12P7/06 , C12N15/52 , C12P17/10 , C12P7/14 , C12P5/02 , C12N9/92
摘要： The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.
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公开(公告)号：US10131923B2
公开(公告)日：2018-11-20
申请号：US15956841
申请日：2018-04-19
申请人： DSM IP ASSETS B.V.
IPC分类号： C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P17/10 , C12P19/02 , C12P19/14 , C12P5/02 , C12P35/00
摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolyzed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
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公开(公告)号：US10118886B2
公开(公告)日：2018-11-06
申请号：US15297610
申请日：2016-10-19
IPC分类号： C07C67/08 , C07C67/297 , C07C29/149 , C07D307/08 , C10L1/02 , C10L1/04 , C10L1/06 , C10G3/00 , C12P7/40 , C07C29/147 , C12P7/48 , C12P7/46 , C07D307/33
摘要： A process for producing biofuels compounds directly from carboxylic acid esters recovered from a fermentation system is described. The process involves taking a fermentation broth that has been reduced to a dry powder containing free organic acids; reacting the carboxylic acid in the powder with an alcohol solvent under a CO2-containing atmosphere in substantial absence of any other acid catalyst at a reaction temperature and pressure that corresponds to supercritical, critical or near critical conditions for at least one of the alcohol or CO2 to synthesize an ester, then subjecting the ester to either hydrogenolysis or hydrogenation to form a biofuel.
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公开(公告)号：US09957528B2
公开(公告)日：2018-05-01
申请号：US14440658
申请日：2013-11-07
申请人： DSM IP ASSETS B.V.
IPC分类号： C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P17/10 , C12P19/02 , C12P19/14 , C12P35/00 , C12P5/02
CPC分类号： C12P7/10 , C12P5/026 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P17/10 , C12P19/02 , C12P19/14 , C12P35/00 , C12P2203/00 , Y02E50/16 , Y02E50/343
摘要： The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.
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