公开(公告)号：US08981046B2

公开(公告)日：2015-03-17

申请号：US13871283

申请日：2013-04-26

Applicant: The Regents of the University of California

Inventor： David V. Schaffer ,  Kevin Edward Healy ,  Lauren Little ,  Patrick Sean Daugherty

IPC: C07K2/00 ,  C07K5/00 ,  C07K17/00 ,  C12N5/0735 ,  G01N33/50 ,  G01N33/542

CPC classification number: C07K17/00 ,  C12N5/0606 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2533/50 ,  C12N2533/90 ,  G01N33/5073 ,  G01N33/542

Abstract: The present invention provides synthetic cell platforms. The synthetic cell platforms can be used for culturing cells in vitro. The synthetic cell platforms can also be implanted together with bound cells into an individual. The present invention provides methods of using the platforms to provide cells or progeny of such cells for use in various applications, including clinical applications; and methods of use of the platforms to introduce cells into an individual.

Abstract translation: 本发明提供合成细胞平台。 合成细胞平台可用于体外培养细胞。 合成细胞平台也可以与结合的细胞一起植入个体中。 本发明提供了使用平台来提供这种细胞的细胞或后代用于各种应用的方法，包括临床应用; 以及使用平台将细胞引入个体的方法。

公开(公告)号：US08883985B2

公开(公告)日：2014-11-11

申请号：US13578974

申请日：2011-02-15

Applicant: Yoichi Kumada ,  Michimasa Kishimoto ,  Kyoko Hamasaki ,  Aya Nakagawa

Inventor： Yoichi Kumada ,  Michimasa Kishimoto ,  Kyoko Hamasaki ,  Aya Nakagawa

IPC: C07K17/02 ,  C07K7/06 ,  C07K7/08 ,  C12N11/06 ,  C12N11/08 ,  G01N33/68 ,  C07K16/00 ,  C07K16/40 ,  C07K16/28 ,  C07K16/18 ,  C07K16/30 ,  C07K16/24

CPC classification number: C07K17/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/249 ,  C07K16/2887 ,  C07K16/303 ,  C07K16/40 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55

Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.

Abstract translation: 本发明提供可用于抗体筛选的抗体固定化载体，产生抗体固定化载体的方法和抗体固定化载体的用途。 特别是通过包含两个或更多个抗体固定化区域的抗体固定化载体，其中分别固定有重链低分子量抗体和轻链低分子量抗体，能够有效地进行抗体筛选， 两个或更多个抗体固定化区域各自以独立的方式包含，重链低分子量抗体包括重链可变区，轻链低分子量抗体包含轻链可变区 ，重链低分子量抗体和轻链低分子量抗体各自衍生自识别不同抗原的抗体。

公开(公告)号：US20140255712A1

公开(公告)日：2014-09-11

申请号：US14240426

申请日：2012-08-24

Applicant: Kai Ostermann ,  Leopold Gruner ,  Gerhard Rödel

Inventor： Kai Ostermann ,  Leopold Gruner ,  Gerhard Rödel

IPC: C07K14/37 ,  B05D3/00

CPC classification number: C07K14/37 ,  B05D3/007 ,  B05D7/24 ,  C07K14/38 ,  C07K17/00 ,  C09D5/00 ,  Y10T428/31725

Abstract: The invention relates to methods for coating a substrate with at least one monolayer of self-assembling proteins, using stabilized aqueous solutions with self-assembling proteins, and also to substrates obtainable as a result. Methods for stabilizing solutions with self-assembling proteins, and the stabilized solutions obtainable therefrom, are likewise provided by the invention. According to the coating method, at least one monolayer of a self-assembling protein is produced on a substrate by first providing a stabilized aqueous solution which comprises at least one self-assembling protein. To provide the coating solution, protein units aggregated from an aqueous solution of self-assembling proteins are separated off, and addition of a solution of ionic surfactants and/or of a salt-containing and/or alkaline and/or acidic solution to the protein-containing coating solution generates monomers or oligomers of the self-assembling proteins, and stabilizes them, the amount of ionic surfactant added being such that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. A substrate surface is then brought into contact with the stabilized, protein-containing solution, thus producing a protein-containing coating on the substrate. The supernatant solution is removed from the coated substrate and/or the coated substrate is dried.

Abstract translation: 本发明涉及使用具有自组装蛋白质的稳定水溶液以及可获得的底物用至少一个单层自组装蛋白质涂覆底物的方法。 本发明同样提供了使用自组装蛋白质稳定溶液的方法及其可获得的稳定化溶液。 根据涂布方法，通过首先提供包含至少一种自组装蛋白质的稳定水溶液，在底物上产生至少一个单层的自组装蛋白质。 为了提供涂布溶液，分离从自组装蛋白质的水溶液聚集的蛋白质单元，并将离子表面活性剂和/或含盐和/或碱性和/或酸性溶液的溶液加入到蛋白质中 包含的涂层溶液产生自组装蛋白质的单体或低聚物并稳定它们，所加入的离子表面活性剂的量使得仅活性蛋白质单体或主要蛋白质低聚物的表面活性部分被表面活性剂颗粒包围。 然后使基底表面与稳定化的含蛋白质溶液接触，从而在基底上产生含蛋白质的涂层。 从涂覆的基底上去除上清液并干燥经涂覆的基底。

公开(公告)号：US08637455B2

公开(公告)日：2014-01-28

申请号：US12739353

申请日：2008-10-17

Applicant: Martyn K. Darby ,  Isaac G. Sanford ,  R. Edward Benson ,  Hanne Gron ,  Paul T. Hamilton ,  Shrikumar A. Nair ,  Doug Buechter ,  Elliott Gruskin

Inventor： Martyn K. Darby ,  Isaac G. Sanford ,  R. Edward Benson ,  Hanne Gron ,  Paul T. Hamilton ,  Shrikumar A. Nair ,  Doug Buechter ,  Elliott Gruskin

IPC: A61K38/04 ,  A01N37/18

CPC classification number: C07K17/00 ,  A61F2310/0097 ,  A61L27/34 ,  A61L27/54 ,  A61L29/085 ,  A61L29/16 ,  A61L31/16 ,  A61L2300/252 ,  A61L2300/406 ,  A61L2300/606 ,  C07K9/00 ,  C07K14/00 ,  C07K2319/00 ,  C08L89/00

Abstract: The presently disclosed subject matter relates to peptides having binding affinity for glycopeptide antibiotics and methods and compositions for delivering glycopeptide antibiotic to the surface of medical devices. The peptide compositions can comprise a peptide having binding affinity for a surface material of a medical device that is coupled to the peptide having binding affinity for glycopeptide antibiotic. Also provided are methods of applying the peptide compositions to a medical device by contacting the peptide compositions with a surface of the medical device. In addition, kits are provided comprising the peptide compositions.

Abstract translation: 目前公开的主题涉及对糖肽抗生素具有结合亲和力的肽以及将糖肽抗生素递送到医疗器械表面的方法和组合物。 肽组合物可以包含对与对糖肽抗生素具有结合亲和力的肽偶联的医疗器械的表面材料具有结合亲和力的肽。 还提供了通过使肽组合物与医疗装置的表面接触来将肽组合物应用于医疗装置的方法。 此外，提供了包含肽组合物的试剂盒。

公开(公告)号：US08501905B2

公开(公告)日：2013-08-06

申请号：US12532098

申请日：2008-03-21

Applicant: David V. Schaffer ,  Kevin Edward Healy ,  Lauren Little ,  Patrick Sean Daugherty

Inventor： David V. Schaffer ,  Kevin Edward Healy ,  Lauren Little ,  Patrick Sean Daugherty

IPC: C07K2/00 ,  C07K5/00

CPC classification number: C07K17/00 ,  C12N5/0606 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2533/50 ,  C12N2533/90 ,  G01N33/5073 ,  G01N33/542

Abstract: The present invention provides synthetic cell platforms. The synthetic cell platforms can be used for culturing cells in vitro. The synthetic cell platforms can also be implanted together with bound cells into an individual. The present invention provides methods of using the platforms to provide cells or progeny of such cells for use in various applications, including clinical applications; and methods of use of the platforms to introduce cells into an individual.

公开(公告)号：US20130122028A1

公开(公告)日：2013-05-16

申请号：US13516385

申请日：2010-12-15

Applicant: Anna-Kariu Olsson ,  Lars Hellman

Inventor： Anna-Kariu Olsson ,  Lars Hellman

IPC: C07K14/78 ,  C07K14/705 ,  C07K14/47

CPC classification number: C07K14/78 ,  A61K39/0011 ,  A61K2039/6068 ,  C07K14/4721 ,  C07K14/705 ,  C07K14/7056 ,  C07K17/00

Abstract: The invention relates to a composition for the treatment of various cancers. The composition is a vaccine containing sequences of EDB, EDA, annexin A1, endosialin, C domain of tenascin C or magic roundabout (MR) or fragments thereof as single or in a combination coupled to one or several heterologous foreign carrier molecules. The vaccine will produce antibodies that are directed against self proteins which are preferentially expressed in and around tumor vessels. The vaccine is preferably administrated together with an adjuvant.

Abstract translation: 本发明涉及用于治疗各种癌症的组合物。 该组合物是含有EDB，EDA，膜联蛋白A1，唾液酸C的内膜糖蛋白，C结构域或神经迂回（MR）或其片段的序列的疫苗，其单独或组合与一个或多个异源外来载体分子偶联。 疫苗将产生针对自身蛋白的抗体，其优先在肿瘤血管内和周围表达。 疫苗优选与佐剂一起施用。

公开(公告)号：US08426179B2

公开(公告)日：2013-04-23

申请号：US12231361

申请日：2008-09-02

Applicant: Phillip B. Messersmith ,  Marsha Ritter-Jones

Inventor： Phillip B. Messersmith ,  Marsha Ritter-Jones

IPC: C12N9/10 ,  A61K38/00 ,  A61K38/04

CPC classification number: C07K17/00 ,  A61K38/00 ,  A61K47/64 ,  C07K5/0812 ,  C07K7/06 ,  C12P21/06 ,  C12Y203/02013

Abstract: Peptides and conjugates thereof comprising one or more bioactive agents which can be coupled to a tissue via a transglutaminase and related methods.

公开(公告)号：US20130085263A1

公开(公告)日：2013-04-04

申请号：US13702673

申请日：2011-06-08

Applicant: Surachai Supattapone ,  Michael B. Miller

Inventor： Surachai Supattapone ,  Michael B. Miller

IPC: C07K17/00

CPC classification number: C07K17/00 ,  B82Y5/00 ,  G01N33/54326 ,  G01N33/6896 ,  G01N2800/2828 ,  Y10S977/705 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/92

Abstract: The present invention is method for non-covalently immobilizing an infectious prion protein using a magnetic substrate.

Abstract translation: 本发明是使用磁性基材非感染性地固定感染性朊蛋白的方法。

公开(公告)号：US20130035283A1

公开(公告)日：2013-02-07

申请号：US13574191

申请日：2011-01-19

Applicant: Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

Inventor： Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

IPC: C07K19/00 ,  A61K38/02 ,  A61P31/00 ,  C12Q1/04

CPC classification number: C07K14/4726 ,  C07K16/44 ,  C07K17/00 ,  C07K2317/53 ,  C07K2317/66 ,  C07K2319/30 ,  G01N33/56961 ,  G01N2333/40

Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.

Abstract translation: 本发明提供可用于结合生物病原体或鉴定用于治疗和诊断感染性疾病，血源性感染或败血症患者的装置和系统的亚类或特定病原体物种的工程化分子调理素。 本发明的一个方面提供了甘露糖结合凝集素（MBL），其是作为先天免疫系统的一部分的丰富的天然血清蛋白。 这种蛋白凝集素在几乎所有类型的生物病原体（病毒，细菌，真菌，原生动物）上结合表面分子的能力使MBL的工程化形式在诊断和治疗感染性疾病和败血症方面非常有用。

公开(公告)号：US08329860B2

公开(公告)日：2012-12-11

申请号：US12443011

申请日：2007-09-27

Applicant: Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K17/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract translation: 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。