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公开(公告)号：US09234847B2
公开(公告)日：2016-01-12
申请号：US12646323
申请日：2009-12-23
Applicant: Patrick Sean Daugherty , Kevin Todd Boulware
Inventor： Patrick Sean Daugherty , Kevin Todd Boulware
CPC classification number: C12N15/1044 , C07K14/245 , C07K2319/03 , C07K2319/22 , C07K2319/60 , C12N15/1037 , C12Q1/37 , C40B40/02 , C40B40/10 , G01N21/6486
Abstract: The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.
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公开(公告)号：US08501905B2
公开(公告)日：2013-08-06
申请号：US12532098
申请日：2008-03-21
Applicant: David V. Schaffer , Kevin Edward Healy , Lauren Little , Patrick Sean Daugherty
Inventor： David V. Schaffer , Kevin Edward Healy , Lauren Little , Patrick Sean Daugherty
CPC classification number: C07K17/00 , C12N5/0606 , C12N2500/99 , C12N2501/115 , C12N2501/15 , C12N2533/50 , C12N2533/90 , G01N33/5073 , G01N33/542
Abstract: The present invention provides synthetic cell platforms. The synthetic cell platforms can be used for culturing cells in vitro. The synthetic cell platforms can also be implanted together with bound cells into an individual. The present invention provides methods of using the platforms to provide cells or progeny of such cells for use in various applications, including clinical applications; and methods of use of the platforms to introduce cells into an individual.
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公开(公告)号：US20120115755A1
公开(公告)日：2012-05-10
申请号：US13284704
申请日：2011-10-28
Applicant: Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakeerthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
Inventor： Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakeerthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
IPC: C40B30/10
CPC classification number: B01L3/502776 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/043 , B03C1/32 , B03C2201/18
Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.
Abstract translation: 微流体装置可以采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 分离由磁电泳驱动。 分选站通常包括单独的缓冲器和采样流。 施加到分选站的磁场梯度将磁性粒子（其选择性地标记目标物种）的流动路径偏转成缓冲液流。 离开分选站的缓冲液流用于检测或进一步处理用磁性颗粒标记的纯化目标物种。
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4.发明申请Cellular Libraries of Peptide Sequences (CLiPS) and Methods of Using the Same 有权肽序列的细胞库（CLiPS）及其使用方法公开(公告)号：US20100173349A1
公开(公告)日：2010-07-08
申请号：US12646323
申请日：2009-12-23
Applicant: Patrick Sean Daugherty , Kevin Todd Boulware
Inventor： Patrick Sean Daugherty , Kevin Todd Boulware
CPC classification number: C12N15/1044 , C07K14/245 , C07K2319/03 , C07K2319/22 , C07K2319/60 , C12N15/1037 , C12Q1/37 , C40B40/02 , C40B40/10 , G01N21/6486
Abstract: The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.
Abstract translation: 本发明提供了包含肽显示支架的组合物，其在至少一个N-末端和C-末端候选肽呈递区域中呈现至少一个候选肽和至少一个可检测部分，当在细胞中表达时， 的细胞外膜。 此外，本发明还提供了用于筛选在肽展示支架中呈现候选肽的细胞文库以鉴定酶的配体的试剂盒和方法。
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公开(公告)号：US20080124779A1
公开(公告)日：2008-05-29
申请号：US11583989
申请日：2006-10-18
Applicant: Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
Inventor： Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
IPC: C12M1/00
CPC classification number: B01L3/502776 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/043 , B03C1/32 , B03C2201/18
Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.
Abstract translation: 微流体装置可以采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 分离由磁电泳驱动。 分选站通常包括单独的缓冲器和采样流。 施加到分选站的磁场梯度将磁性粒子（其选择性地标记目标物种）的流动路径偏转成缓冲液流。 离开分选站的缓冲液流用于检测或进一步处理用磁性颗粒标记的纯化目标物种。
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Patent Agency Ranking
公开(公告)号：US20120122731A1
公开(公告)日：2012-05-17
申请号：US11655055
申请日：2007-01-17
Applicant: Hyongsok Soh , Patrick Sean Daugherty , Sang-Hyun Oh
Inventor： Hyongsok Soh , Patrick Sean Daugherty , Sang-Hyun Oh
CPC classification number: B03C1/286 , B01J2219/005 , B01J2219/00563 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00648 , B01J2219/00655 , B01J2219/00707 , B01L3/502761 , B01L3/502776 , B01L2200/0652 , B01L2300/0864 , B01L2400/043 , B01L2400/0487 , B03C1/30 , B03C2201/18
Abstract: Screening in a microfluidic device is mediated by a magnetic field that in some manner displaces or otherwise activates the entities of interest. Entities of interest can be identified and/or separated from one or more other components provided to the microfluidic device. Microfluidic devices may have mechanisms that apply a defined magnetic field to a region of the microfluidic device where library members pass through sequentially and/or in parallel.
Abstract translation: 在微流体装置中的筛选由磁场介导，磁场以某种方式置换或以其他方式激活感兴趣的实体。 感兴趣的实体可以被识别和/或从提供给微流体装置的一个或多个其它组件分离。 微流体装置可以具有将定义的磁场施加到微流体装置的区域的机制，其中库构件顺序地和/或并行穿过。
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公开(公告)号：US08071054B2
公开(公告)日：2011-12-06
申请号：US12871788
申请日：2010-08-30
Applicant: Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
Inventor： Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
IPC: C12Q1/70
CPC classification number: B01L3/502776 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/043 , B03C1/32 , B03C2201/18
Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.
Abstract translation: 微流体装置可以采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 分离由磁电泳驱动。 分选站通常包括单独的缓冲器和采样流。 施加到分选站的磁场梯度将磁性粒子（其选择性地标记目标物种）的流动路径偏转成缓冲液流。 离开分选站的缓冲液流用于检测或进一步处理用磁性颗粒标记的纯化目标物种。
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公开(公告)号：US07807454B2
公开(公告)日：2010-10-05
申请号：US11583989
申请日：2006-10-18
Applicant: Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
Inventor： Sang-Hyun Oh , Amarendra Kumar Singh , Yanting Zhang , Dharmakirthi Nawarathna , Unyoung Kim , Patrick Sean Daugherty , Hyongsok Soh , Brian Scott Ferguson
IPC: G01N33/553
CPC classification number: B01L3/502776 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/043 , B03C1/32 , B03C2201/18
Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.
Abstract translation: 微流体装置可以采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 分离由磁电泳驱动。 分选站通常包括单独的缓冲器和采样流。 施加到分选站的磁场梯度将磁性粒子（其选择性地标记目标物种）的流动路径偏转成缓冲液流。 离开分选站的缓冲液流用于检测或进一步处理用磁性颗粒标记的纯化目标物种。
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公开(公告)号：US20100189760A1
公开(公告)日：2010-07-29
申请号：US12532098
申请日：2008-03-21
Applicant: David V. Schaffer , Kevin Edward Healy , Lauren Little , Patrick Sean Daugherty
Inventor： David V. Schaffer , Kevin Edward Healy , Lauren Little , Patrick Sean Daugherty
CPC classification number: C07K17/00 , C12N5/0606 , C12N2500/99 , C12N2501/115 , C12N2501/15 , C12N2533/50 , C12N2533/90 , G01N33/5073 , G01N33/542
Abstract: The present invention provides synthetic cell platforms. The synthetic cell platforms can be used for culturing cells in vitro. The synthetic cell platforms can also be implanted together with bound cells into an individual. The present invention provides methods of using the platforms to provide cells or progeny of such cells for use in various applications, including clinical applications; and methods of use of the platforms to introduce cells into an individual.
Abstract translation: 本发明提供合成细胞平台。 合成细胞平台可用于体外培养细胞。 合成细胞平台也可以与结合的细胞一起植入个体中。 本发明提供了使用平台来提供这种细胞的细胞或后代用于各种应用的方法，包括临床应用; 以及使用平台将细胞引入个体的方法。
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公开(公告)号：US08541203B2
公开(公告)日：2013-09-24
申请号：US13411416
申请日：2012-03-02
Applicant: Patrick Sean Daugherty , Nancy Stagliano , Jerry Thomas , James W. West , Jason Sagert
Inventor： Patrick Sean Daugherty , Nancy Stagliano , Jerry Thomas , James W. West , Jason Sagert
CPC classification number: C07K16/22 , C07K16/24 , C07K16/2818 , C07K16/2836 , C07K16/42 , C07K2317/34 , C07K2317/622 , C07K2319/00 , C07K2319/30 , C07K2319/50 , C12N15/1034 , C12N15/1044
Abstract: Activatable binding polypeptides (ABPs), which contain a target binding moiety (TBM), a masking moiety (MM), and a cleavable moiety (CM) are provided. Activatable antibody compositions, which contain a TBM containing an antigen binding domain (ABD), a MM and a CM are provided. Furthermore, ABPs which contain a first TBM, a second TBM and a CM are provided. The ABPs exhibit an “activatable” conformation such that at least one of the TBMs is less accessible to target when uncleaved than after cleavage of the CM in the presence of a cleaving agent capable of cleaving the CM. Further provided are libraries of candidate ABPs, methods of screening to identify such ABPs, and methods of use. Further provided are ABPs having TBMs that bind VEGF, CTLA-4, or VCAM, ABPs having a first TBM that binds VEGF and a second TBM that binds FGF, as well as compositions and methods of use.
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