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11.发明申请公开(公告)号：US20150166956A1
公开(公告)日：2015-06-18
申请号：US14167393
申请日：2014-01-29
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Erik Leeming Kvam , Gregory Andrew Grossmann , Craig Patrick Galligan , Jason Michael Nichols , Xuefeng Wang , Jason Louis Davis
IPC: C12N5/078
CPC classification number: B01D21/0087 , B01D17/02 , B01D21/0006 , B01D21/0042 , B01D21/02 , B01D21/10 , B01D21/2433 , B01D21/2444 , B01D21/245 , B01D2221/04 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2300/0681 , B01L2300/0851 , B01L2400/0421 , B01L2400/043 , B01L2400/0457 , B01L2400/0469 , C02F1/00 , C02F1/24 , C02F1/444 , C02F1/48 , C02F2001/007 , C02F2101/32 , C02F2103/08 , C02F2103/10 , C02F2201/002 , C12M47/02 , C12N5/0641
Abstract: A device is configured for separation of particulates dispersed within a base fluid, wherein the particulates have a relative density difference compared to the base fluid. The device comprises a microchannel of length l and height h comprising an inlet and an outlet; a microporous surface on one or more walls of the microchannel; a collection chamber on an opposing side of the microporous surface; and an applied force field across the height h of the microchannel to sediment the particles through the microporous surface into the collection chamber. The microporous body operationally generates a fluid flow regime comprising a first fluid flow having a first flow rate through the microchannel and a second fluid flow having a second flow rate through the collection chamber and the second flow rate is a fraction of the first flow rate.
Abstract translation: 一种装置被配置用于分离分散在基础流体中的微粒，其中所述颗粒与基础流体相比具有相对密度差。 该装置包括长度为l的微通道和包括入口和出口的高度h; 在微通道的一个或多个壁上的微孔表面; 在微孔表面的相对侧上的收集室; 以及穿过微通道的高度h的施加的力场，以将颗粒通过微孔表面沉淀到收集室中。 微孔体可操作地产生流体流动状态，其包括具有通过微通道的第一流量的第一流体流和具有通过收集室的第二流速的第二流体流，第二流量是第一流量的一部分。
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12.发明授权公开(公告)号：US11726078B2
公开(公告)日：2023-08-15
申请号：US17091501
申请日：2020-11-06
Applicant: General Electric Company , Tokitae LLC
Inventor： Matthew Jeremiah Misner , Gregory Andrew Grossmann , Cathryn Ellen Olsen , John Richard Nelson , David Roger Moore , Paul Michael Smigelski, Jr. , John Thomas Connelly , Benjamin David Grant , Bernhard Hans Weigl
IPC: G01N33/493 , C07K1/22 , B01D15/08 , G01N33/50 , G01N33/569 , B01D63/02 , A61B10/00 , C12M1/26 , G01N33/558 , G01N15/14
CPC classification number: G01N33/493 , A61B10/007 , B01D15/08 , B01D63/02 , C07K1/22 , C12M33/14 , G01N33/5038 , G01N33/558 , G01N33/569 , G01N33/5695 , A61M2202/005 , A61M2202/0028 , A61M2202/0071 , A61M2202/0496 , G01N2015/1493
Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.
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13.发明授权公开(公告)号：US11714084B2
公开(公告)日：2023-08-01
申请号：US17090383
申请日：2020-11-05
Applicant: General Electric Company
Inventor： Matthew Jeremiah Misner , Gregory Andrew Grossmann , Cathryn Ellen Olsen , John Richard Nelson , Brian Christopher Bales , David Roger Moore , Paul Michael Smigelski, Jr.
IPC: G01N33/569 , G01N1/40 , G01N33/543 , B01L3/00 , B01D15/36
CPC classification number: G01N33/5695 , B01D15/363 , B01L3/5023 , G01N1/4005 , G01N33/54366 , B01L2200/0631 , B01L2200/10 , B01L2300/0825 , G01N2001/4011 , G01N2333/35 , G01N2405/06 , G01N2469/10
Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.
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公开(公告)号：US09534214B2
公开(公告)日：2017-01-03
申请号：US14068633
申请日：2013-10-31
Applicant: General Electric Company
Inventor： Bing Li , Gregory Andrew Grossmann , Erik Leeming Kvam , Brian Christopher Bales , Jason Louis Davis
CPC classification number: C12N15/1006 , B01J20/22 , B01J20/261 , B01J20/321 , B01J20/3212 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3274 , C12N15/1017
Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.
Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体底物涂覆有糖，例如单糖，寡糖，多糖或其组合，并且底物由一种或多种在基本干燥状态下浸渍的蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。
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Patent Agency Ranking
公开(公告)号：US20150119566A1
公开(公告)日：2015-04-30
申请号：US14068633
申请日：2013-10-31
Applicant: General Electric Company
Inventor： Bing Li , Gregory Andrew Grossmann , Erik Leeming Kvam , Brian Christopher Bales , Jason Louis Davis
CPC classification number: C12N15/1006 , B01J20/22 , B01J20/261 , B01J20/321 , B01J20/3212 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3274 , C12N15/1017
Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.
Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体底物涂覆有糖，例如单糖，寡糖，多糖或其组合，并且底物由一种或多种在基本干燥状态下浸渍的蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。
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16.发明申请METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS 有权用于收集和放大循环核酸的方法和装置公开(公告)号：US20150031035A1
公开(公告)日：2015-01-29
申请号：US13952173
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew Grossmann , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
IPC: C12Q1/68
CPC classification number: C12N15/1017 , B01L7/52 , B01L2300/0681 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
Abstract translation: 本文提供了从生物样品的非细胞部分收集和扩增循环核酸的方法。 从非细胞级分提取循环核酸，并进行环化以产生单链核酸环，然后通过使用随机引物的滚动循环扩增产生扩增文库，然后扩增其。 还提供了用于从生物样品中收集非细胞级分的装置。 该装置包括与过滤膜直接接触的过滤膜和干燥固体基质。
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公开(公告)号：US10472620B2
公开(公告)日：2019-11-12
申请号：US14321160
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
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公开(公告)号：US20180345278A1
公开(公告)日：2018-12-06
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: B01L3/00 , C12Q1/02 , G01N21/64 , G01N21/76 , G01N33/554
CPC classification number: C12Q1/02 , C12M41/00 , C12M41/46 , C12Q1/18 , G01N21/6408 , G01N21/6452 , G01N33/4836
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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公开(公告)号：US20150118683A1
公开(公告)日：2015-04-30
申请号：US14068532
申请日：2013-10-31
Applicant: General Electric Company
Inventor： Bing Li , Gregory Andrew Grossmann , David Roger Moore
IPC: C12Q1/68
CPC classification number: C12N15/1006
Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate comprises a surface modified with a plurality of hydrophilic groups; and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.
Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体基质包括用多个亲水基团改性的表面; 并且底物由在基本干燥状态下浸渍的一种或多种蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。
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