公开(公告)号：US12129512B2

公开(公告)日：2024-10-29

申请号：US18677555

申请日：2024-05-29

申请人： Roche Sequencing Solutions, Inc.

发明人： Garry P. Nolan

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

CPC分类号： C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

摘要： Provided herein is a composition comprising a mixture of barcoded nucleic acid molecules made from a plurality of cells or cell organelles, wherein the mixture comprises: (a) a population of first nucleic acid molecules each comprising: a sequence of a nucleic acid from a cell or cell organelle, a complement of the sequence, or a barcode identifying the sequence, and a cell-origination barcode; and (b) a population of second nucleic acid molecules each comprising: an epitope specific barcode and a cell-origination barcode. In this composition, the first and second nucleic acid molecules from the same cell or cell organelle have the same cell-origination barcode and first and second nucleic acid molecules from different cells or cell organelles have different cell-origination barcodes.

公开(公告)号：US20240352513A1

公开(公告)日：2024-10-24

申请号：US18753991

申请日：2024-06-25

申请人： Natera, Inc.

发明人： Joshua BABIARZ ,  Tudor Pompiliu CONSTANTIN ,  Lane A. EUBANK ,  George GEMELOS ,  Matthew Micah HILL ,  Huseyin Eser KIRKIZLAR ,  Matthew RABINOWITZ ,  Onur SAKARYA ,  Styrmir SIGURJONSSON ,  Bernhard ZIMMERMANN

IPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883

CPC分类号： C12Q1/686 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6848 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US12123052B2

公开(公告)日：2024-10-22

申请号：US17130394

申请日：2020-12-22

申请人： Cepheid

发明人： Joseph H. Smith ,  David H. Persing ,  Alan Wortman ,  Ronald Chang ,  David Swenson

IPC分类号： C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689

CPC分类号： C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/689 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0644 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2547/101 ,  C12Q2537/149 ,  C12Q1/6848 ,  C12Q2565/629 ,  C12Q2565/101 ,  C12Q2527/107 ,  C12Q1/6851 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2537/143

摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

公开(公告)号：US12123015B2

公开(公告)日：2024-10-22

申请号：US17481085

申请日：2021-09-21

申请人： The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  A01H6/46 ,  A01K67/027 ,  A61K38/46 ,  A61K48/00 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12Q1/686 ,  H01L21/02 ,  H01L33/00

CPC分类号： C12N15/907 ,  A01H6/4684 ,  A01K67/027 ,  A61K38/465 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/746 ,  C12N15/90 ,  C12N15/902 ,  C12Q1/686 ,  H01L21/02312 ,  H01L21/02365 ,  H01L33/0075 ,  A61K48/00 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/80 ,  C12Y301/04

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US12122859B2

公开(公告)日：2024-10-22

申请号：US18101785

申请日：2023-01-26

申请人： LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

发明人： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC分类号： C08F230/08 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F130/08 ,  C08F220/58 ,  C08F222/38 ,  C08J9/20 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/414 ,  C08F220/60

CPC分类号： C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F222/385 ,  C08F230/085 ,  C08J9/20 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  C08F220/603 ,  C08F2800/20 ,  C08J2343/04 ,  Y10T428/2982 ,  C08F8/12 ,  C08F257/02 ,  C08F8/34 ,  C08F8/12 ,  C08F257/02 ,  C12Q1/686 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543 ,  C08F257/02 ,  C08F230/085 ,  C08F8/12 ,  C08F230/085 ,  C08F8/34 ,  C08F8/12 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F230/085 ,  C08F220/603 ,  C08F230/085 ,  C08F212/36 ,  C08F230/085 ,  C08F222/102

摘要： A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20240344117A1

公开(公告)日：2024-10-17

申请号：US18683200

申请日：2022-08-15

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Kerry GEILER-SAMEROTTE ,  Kara SCHMIDLIN ,  Leandra BRETTNER

IPC分类号： C12Q1/6841 ,  C12N15/10 ,  C12Q1/686

CPC分类号： C12Q1/6841 ,  C12N15/1065 ,  C12Q1/686

摘要： Disclosed herein is an in situ, high throughput, single-cell whole-genome sequencing technology developed for sequencing genomes in large heterogeneous cell populations. More specifically, the invention disclosed herein does not require cell sorting or isolation because it uses the cell membrane to separate each genome.

公开(公告)号：US12116578B2

公开(公告)日：2024-10-15

申请号：US16988025

申请日：2020-08-07

申请人： APDN (B.V.I.) Inc.

发明人： Michael E. Hogan ,  Stephen Hughes ,  Yuhua Sun

IPC分类号： C12N15/70 ,  C12N7/00 ,  C12Q1/686

CPC分类号： C12N15/70 ,  C12N7/00 ,  C12Q1/686 ,  C12N2750/14143 ,  C12N2750/14151

摘要： The present invention relates to systems and methods to produce recombinant adeno-associated virus (rAAV) utilizing one or more DNA constructs manufactured via polymerase chain reaction (PCR).

公开(公告)号：US12110544B2

公开(公告)日：2024-10-08

申请号：US17058964

申请日：2018-07-13

申请人： INSTITUTE OF ANIMAL SCIENCES, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Jiaqi Wang ,  Shengguo Zhao ,  Nan Zheng ,  Xu Zhou ,  Songli Li

IPC分类号： C12Q1/686 ,  C12N15/10 ,  C12Q1/6806

CPC分类号： C12Q1/686 ,  C12N15/1003 ,  C12Q1/6806

摘要： In a method for extracting microbial DNA from milk, the extracted DNA can be directly used for PCR amplification. The method includes the following steps: treating milk with TE solution, fully disrupting microbial cells by bead milling, immobilizing proteins in the middle layer between the aqueous and organic phases with high-vacuum silicone grease so as to prevent impurities in the middle layer from entering the aqueous phase, etc. The extracted DNA has a high degree of intactness, high concentration and purity.

公开(公告)号：US12110535B2

公开(公告)日：2024-10-08

申请号：US18385300

申请日：2023-10-30

申请人： Gen-Probe Incorporated

发明人： David A. Buse ,  Norbert D. Hagen ,  David Opalsky

IPC分类号： C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/686 ,  G01N35/04

CPC分类号： C12Q1/6806 ,  B01L3/50825 ,  B01L3/565 ,  B01L7/5255 ,  C12Q1/686 ,  G01N35/04 ,  B01L3/527 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2300/046 ,  G01N2035/0418 ,  G01N2035/0436

摘要： A method for reconstituting a reagent within a reagent pack including one or more wells and at least one of the wells containing a lyophilized reagent comprises the steps of receiving reagent packs into a reagent pack input carousel, transferring one reagent pack with a rotary distributor from the input carousel to a reagent pack storage carousel, retrieving the reagent pack from the storage carousel with the rotary distributor and placing the reagent pack into a reagent pack loading station, and dispensing a reconstitution reagent with an automated pipettor into the well containing the lyophilized reagent to form a reconstituted reagent.

公开(公告)号：US20240331799A1

公开(公告)日：2024-10-03

申请号：US18585708

申请日：2024-02-23

申请人： Natera, Inc.

发明人： MATTHEW RABINOWITZ ,  George GEMELOS ,  Milena BANJEVIC ,  Allison RYAN ,  Zachary DEMKO ,  Matthew Hill ,  Bernhard ZIMMERMANN ,  Johan BANER

IPC分类号： G16B20/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6883 ,  G16B20/10 ,  G16B20/20 ,  G16B20/40 ,  G16B40/00

CPC分类号： G16B20/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6883 ,  G16B20/10 ,  G16B20/20 ,  G16B20/40 ,  C12Q2525/179 ,  C12Q2527/113 ,  C12Q2527/143 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2537/159 ,  C12Q2545/114 ,  C12Q2600/156 ,  C12Q2600/16 ,  G16B40/00

摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.