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公开(公告)号:US12116578B2
公开(公告)日:2024-10-15
申请号:US16988025
申请日:2020-08-07
申请人: APDN (B.V.I.) Inc.
发明人: Michael E. Hogan , Stephen Hughes , Yuhua Sun
CPC分类号: C12N15/70 , C12N7/00 , C12Q1/686 , C12N2750/14143 , C12N2750/14151
摘要: The present invention relates to systems and methods to produce recombinant adeno-associated virus (rAAV) utilizing one or more DNA constructs manufactured via polymerase chain reaction (PCR).
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公开(公告)号:US10745825B2
公开(公告)日:2020-08-18
申请号:US16028176
申请日:2018-07-05
申请人: APDN (B.V.I.) INC.
IPC分类号: B29C65/00 , B29C65/48 , B29C71/00 , B32B37/00 , B41M3/14 , G07D7/14 , C40B20/04 , G07D7/1205 , B42D25/00 , C09B11/22 , C09B11/24 , C09B69/10 , C09B11/08 , C12Q1/68 , C09B23/16 , C09B57/10 , G07D7/202 , C12Q1/6876
摘要: Encrypted markers that are not readily detectable can be revealed by treatment with a specific reagent used as a developer to reveal a readily detectable physical property of the marker, such as a characteristic fluorescence emission after excitation with a particular excitation wavelength, or to reveal a visible color. The encrypted marker can be developed in situ, or a sample can be removed by brushing, scraping, swabbing or scratching the marked object or item and developing the encrypted marker or a sample thereof with the appropriate developer to reveal an overt marker or optical signal. The encrypted marker may include a DNA taggant.
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公开(公告)号:US20180194796A1
公开(公告)日:2018-07-12
申请号:US15870035
申请日:2018-01-12
申请人: APDN (B.V.I.) INC.
IPC分类号: C07H21/04 , C05C1/00 , C07C211/03 , C07C229/16 , C12Q1/686 , C01G49/00
CPC分类号: C07H21/04 , C01G49/00 , C05C1/00 , C05D9/02 , C05G3/00 , C05G3/0035 , C05G3/0041 , C07C211/03 , C07C229/16 , C12Q1/6816 , C12Q1/686 , C12Q2563/185
摘要: The invention pertains to a traceable fertilizer product marked with a nucleic acid marker. The invention further provides for a method of marking a fertilizer product with a nucleic acid marker. In addition, the invention teaches a method of using a nucleic acid marked fertilizer product to identify and validate the origin or authenticity of said fertilizer product and to perform quantitative analysis on the fertilizer product to determine if said fertilizer product has been adulterated, diluted and/or counterfeited.
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公开(公告)号:US20180173810A1
公开(公告)日:2018-06-21
申请号:US15868510
申请日:2018-01-11
申请人: APDN (B.V.I.) INC.
发明人: Judith Murrah , Phidung H. Tran , Maciej B. Szczepanik , MingHwa Benjamin Liang , James A. Hayward
CPC分类号: G06F17/30876 , G06K7/0004 , G06K19/06037 , G06K19/0723
摘要: A system associated with authentication of an object related to a multi-mode marker using a processing device. The processing device performs operations that include detecting a signal associated with a multi-mode marker related with the object; assigning a digital code based on the detected signal associated with the multi-mode marker; and identifying the object based on the digital code related to authentication of the object. A corresponding method and computer-readable device are also disclosed.
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公开(公告)号:US09904734B2
公开(公告)日:2018-02-27
申请号:US15027454
申请日:2014-10-07
申请人: APDN (B.V.I.) INC.
发明人: Judith Murrah , Phidung H. Tran , Maciej B. Szczepanik , MingHwa Benjamin Liang , James A. Hayward
CPC分类号: G06F17/30876 , G06K7/0004 , G06K19/06037 , G06K19/0723
摘要: A multi-mode reader instrument capable of detecting and determining digital data from a signal from one or more markers, indicia or taggants on an object, the markers, indicia or taggants such as a bar code, a QR code, an RFID, an optical compound, a fluorescent compound, a phosphorescent compound, a DNA taggant, an upconverting phosphor (UCP), a chemical dye, a digitized image, a radioactive compound, an olfactory compound or a thermal attribute of the object is provided. Also, provided is a method and a system for identifying an object, the system includes: a multi-mode reader instrument for detecting data from a signal from one or more markers, indicia or taggants on an object and assignment of digital code for the marker and a database for securing and retrieving information relevant to the item.
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公开(公告)号:US20170292206A1
公开(公告)日:2017-10-12
申请号:US15466016
申请日:2017-03-22
申请人: APDN (B.V.I.) Inc.
摘要: Methods for marking cellulosic products, including cellulosic fibers such as lyocell and cellulosic films, including methods for marking such products with a detectable nucleic acid marker to identify and validate the origin or authenticity of the products or items manufactured using such products. Detectably-marked cellulosic products marked with nucleic acid markers for authentication, validation and tracking are also provided.
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公开(公告)号:US20170021611A1
公开(公告)日:2017-01-26
申请号:US15049170
申请日:2016-02-22
申请人: APDN (B.V.I.) Inc.
CPC分类号: B41F17/00 , B41M3/14 , B42D25/378 , B42D25/405 , C12Q1/68 , G07D7/14 , Y10T29/49826 , Y10T29/49885
摘要: The invention provides a method of marking an item with naturally-derived or synthetic non-natural polymeric marker molecules, such as a DNA or Peptide marker in conjunction with optional visible or rapid scan reporters for authenticating or tracking, in which the method includes providing an item for marking, and applying a medium including a DNA marker to the item. The invention further provides methods for stably binding and immobilizing activated deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. Also provided are methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. The invention also provides a method of marking an item with a DNA marker for authenticating or tracking, in which the method includes providing a medium including a DNA marker, and molding the medium including the DNA marker to provide all or part of the item. The DNA marker encodes information unique to the item and/or the model of the item as desired.
摘要翻译: 本发明提供了一种使用天然衍生或合成的非天然聚合物标记分子(例如DNA或肽标记)与用于鉴定或跟踪的可选的可见或快速扫描记录器一起标记物品的方法,其中所述方法包括提供 用于标记的项目,以及将包括DNA标记的培养基应用于该物品。 本发明还提供了将活化的脱氧核糖核酸稳定结合并固定在物体和底物上的方法。 该方法包括将脱氧核糖核酸暴露于碱性条件下,并使脱氧核糖核酸与对象或底物接触。 还提供了用于增加标签从对象的可恢复性的方法。 所述方法包括将标签剂掺入溶液中的步骤; 将包含标签剂的溶液与扰动剂混合以形成第一扰动标记剂溶液; 将第一扰动标记剂溶液与聚合物混合以形成第二扰动标记聚合物溶液; 以及将所述第二扰动标记聚合物溶液施加到所述物体的至少一部分以形成标记剂涂覆物体。 本发明还提供了用用于鉴定或追踪的DNA标记物标记物品的方法,其中所述方法包括提供包含DNA标记物的培养基,并且模制包含DNA标记的培养基以提供该物品的全部或部分。 DNA标记根据需要编码项目和/或项目模型唯一的信息。
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公开(公告)号:US20150266332A1
公开(公告)日:2015-09-24
申请号:US14661489
申请日:2015-03-18
申请人: APDN (B.V.I.) INC.
IPC分类号: B42D25/415 , B42D25/36
CPC分类号: C09K11/06 , B29C65/4825 , B29C66/733 , B41M3/142 , B41M3/144 , B42D2035/34 , G07D7/14
摘要: Encrypted markers that are not readily detectable can be revealed by treatment with a specific reagent used as a developer to reveal a readily detectable physical property of the marker, such as a characteristic fluorescence emission after excitation with a particular excitation wavelength, or to reveal a visible color. The encrypted marker can be developed in situ, or a sample can be removed by brushing, scraping, swabbing or scratching the marked object or item and developing the encrypted marker or a sample thereof with the appropriate developer to reveal an overt marker or optical signal. The marker can be revealed by exposure of the encrypted marker or a sample thereof to the developer in any suitable form, such as a solution, a slurry, a swab, a solid (such as in granular form), or a gas or a vapor that includes a developer.
摘要翻译: 通过用用作显色剂的特定试剂进行处理,可以显示不容易检测到的加密标记物,以显示标记的易于检测的物理性质,例如用特定激发波长激发后的特征性荧光发射,或显示可见光 颜色。 可以原位开发加密的标记,或者可以通过刷涂,刮擦,擦拭或刮擦标记的物体或物品并用合适的显影剂开发加密标记或样品,以揭示公开标记或光信号来移除样品。 可以通过将加密的标记物或其样品以任何合适的形式(例如溶液,浆液,拭子,固体(例如颗粒形式))或气体或蒸汽暴露于显影剂来显示标记 包括开发人员。
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公开(公告)号:US09005985B2
公开(公告)日:2015-04-14
申请号:US13761447
申请日:2013-02-07
申请人: APDN (B.V.I.) Inc.
CPC分类号: C12Q1/6876 , B82Y15/00 , C08K5/09 , C09D7/63 , C09D11/03 , C12Q1/6816 , Y10S977/774 , Y10S977/92 , Y10T436/14 , Y10T436/143333 , C12Q2563/185
摘要: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
摘要翻译: 本发明提供具有与生物分子连接的发光报道基团的组合物,优选核苷酸寡聚体。 在将生物分子共价连接到光报告颗粒之前,光报告颗粒被甲硅烷基化和官能化以产生包被的光报告颗粒。 本发明的光报告颗粒可以通过诸如UV或IR光的光激发源激发,并且当生物分子是DNA时,通过诸如PCR的扩增技术检测所连接的DNA分子。
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公开(公告)号:US20210353676A1
公开(公告)日:2021-11-18
申请号:US17373194
申请日:2021-07-12
申请人: APDN (B.V.I.), Inc.
IPC分类号: A61K35/17 , C12N5/0781 , A61P35/00 , C12N5/0783 , C12Q1/686 , C12Q1/6897
摘要: The present inventions provides systems and methods to manufacture genetically modified lymphocyte cells via the use of linear DNA expression amplicons, and uses of such genetically modified lymphocyte cells to treat disease. The present invention also provides for the composition of genetically modified lymphocyte cells.
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