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    Pharmaceutically compatible method for purifying intact bacterial minicells
    1.
    发明申请
    Pharmaceutically compatible method for purifying intact bacterial minicells 有权
    用于纯化完整细菌细菌的药物相容性方法

    公开(公告)号：US20040265994A1

    公开(公告)日：2004-12-30

    申请号：US10602021

    申请日：2003-06-24

    申请人： EnGeneIC Therapy Pty. Ltd.

    发明人： Himanshu Brahmbhatt ,  Jennifer MacDiarmid

    IPC分类号： C12N001/20 ,  C12N005/02

    CPC分类号： A61K35/74 ,  C12N1/02 ,  C12N1/20

    摘要： The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.

    摘要翻译： 本发明提供了一种纯化细菌微细胞的方法，该方法包括在生物相容性培养基中使含有微细菌的样品进行密度梯度离心。 该方法可选地包括初步差速离心步骤和一个或多个过滤步骤。 本发明还提供了一种纯化细菌微细胞的方法，其中含有微细胞的样品经受诱导亲本细菌细胞采用丝状形式的条件，然后过滤样品以从细菌细胞分离小细胞。 本发明的方法任选地包括从纯化的微细胞制剂中除去内毒素和/或用抗生素处理纯化的微细胞制剂的一个或多个步骤。 另外，本发明提供了根据前述方法制备的纯化小细胞制剂，每10 7,10,8，10 9，10 10或10 10个细菌含有少于约1个污染的亲本细菌细胞， 11>小细胞。

    Egg freezing and storing tool and method
    2.
    发明申请
    Egg freezing and storing tool and method 有权
    蛋冻存储工具及方法

    公开(公告)号：US20040259072A1

    公开(公告)日：2004-12-23

    申请号：US10824605

    申请日：2004-04-15

    申请人： Kabushiki Kaisha KITAZATO SUPPLY

    发明人： Masashige Kuwayama ,  Futoshi Inoue

    IPC分类号： A01N001/00 ,  A01N001/02 ,  C12N005/00 ,  C12N005/08 ,  C12N005/02 ,  F25B039/02

    CPC分类号： A01N1/02 ,  A01N1/0268 ,  A61D19/022

    摘要： An egg freezing and storing instrument has an egg freezing and storing tube made of a liquid nitrogen-resistant material; and a metal cylindrical protection member for protecting the tube. The tube has a body part; and an egg-storing small-diameter part having an inner diameter of 0.1 mm to 0.5 mm. The tube can be heat-sealed at a front side of the small-diameter part and at a rear side of the body part. The cylindrical protection member has a tubular part for accommodating a front side of the small-diameter part of the tube; and a semi-tubular part for accommodating a portion of the small-diameter part not accommodated in the tubular part and a front portion of the body part.

    摘要翻译： 鸡蛋冷冻储存器具有一个由防液体材料制成的鸡蛋冷冻储存管; 以及用于保护管的金属圆柱形保护构件。 管具有身体部分; 以及内径为0.1mm〜0.5mm的储蛋小口径部。 管可以在小直径部分的前侧和身体部分的后侧被热密封。 圆柱形保护构件具有用于容纳管的小直径部分的前侧的管状部分; 以及用于容纳未容纳在管状部分中的小直径部分的一部分和身体部分的前部的半管状部分。

    Method of manufacturing a culture medium
    3.
    发明申请
    Method of manufacturing a culture medium 审中-公开
    培养基的制造方法

    公开(公告)号：US20040235168A1

    公开(公告)日：2004-11-25

    申请号：US10782419

    申请日：2004-02-17

    申请人： Van der Sluis Cigar Machinery B.V.

    发明人： Lucas Everhardus Maria Langezaal

    IPC分类号： C12N005/02

    CPC分类号： A01G24/00

    摘要： The invention relates to a method of manufacturing a culture medium on which plants can be grown. The method comprises mixing organic and/or inorganic particulate base materials with a thermoplastic, biologically degradable, binding agent, heating the mixture to make it at least semi-fluid, and then cooling the mixture to solidify it.

    摘要翻译： 本发明涉及一种制造可在其上生长植物的培养基的方法。 该方法包括将有机和/或无机颗粒基材与热塑性，生物可降解的结合剂混合，加热混合物至少使其成为半流体，然后冷却混合物以使其固化。

    Method and device for the in vitro cultivation of cells
    4.
    发明申请
    Method and device for the in vitro cultivation of cells 审中-公开
    细胞体外培养的方法和装置

    公开(公告)号：US20040219668A1

    公开(公告)日：2004-11-04

    申请号：US10488018

    申请日：2004-02-27

    发明人： Heribert Frei ,  Pierre Mainil-Varlet ,  Werner Muller

    IPC分类号： C12N005/08 ,  C12N005/02

    CPC分类号： C12M25/02 ,  C12M33/00

    摘要： A method for in vitro cultivation of cells, which grow on a culture surface, wherein the cells are sown on culture surfaces (1) and are cultivated in a culture medium, whereby the culture surface is continually or periodically expanded, without being removed from the culture medium. To expand the culture surface, the cells are not detached from the culture surface and the culture surface between the cells is expanded. However, at least part of the cells can be detached and the culture surface can be expanded by the flooding of additional culture surface areas. The culture surface of an example device for cell cultivation consists of one side of an expanding membrane (6), which is expanded by modifying the pressure on the other side. The cell cultivation can be carried out without manual passaging. The cells are subjected to less stress than in known cell cultivation methods and the method can be automated more easily.

    摘要翻译： 一种体外培养在培养物表面生长的细胞的方法，其中将细胞播种在培养表面（1）上，并在培养基中培养，由此培养物表面不断地或周期性地扩大，而不从 培养基。 为了扩大培养物表面，细胞不脱离培养物表面，细胞间的培养面积扩大。 然而，至少部分细胞可以分离，并且培养物表面可以通过附加培养表面积的淹没而扩大。 用于细胞培养的示例性装置的培养表面由膨胀膜（6）的一侧组成，其通过改变另一侧的压力而膨胀。 细胞培养可以在没有手动传代的情况下进行。 细胞受到比已知细胞培养方法更少的压力，并且该方法可以更容易地自动化。

    Plasma protein matrices and methods for their preparation
    5.
    发明申请
    Plasma protein matrices and methods for their preparation 有权
    血浆蛋白质基质及其制备方法

    公开(公告)号：US20040209359A1

    公开(公告)日：2004-10-21

    申请号：US10761615

    申请日：2004-01-20

    发明人： Avner Yayon ,  Rachel Glicklis

    IPC分类号： C12N005/02

    CPC分类号： A61L27/3852 ,  A61K35/12 ,  A61L27/225 ,  A61L27/3839 ,  A61L27/56 ,  C12N5/0068 ,  C12N5/0655 ,  C12N2533/56

    摘要： A freeze-dried biocompatible matrix that includes plasma proteins that are useful as implants for tissue engineering as well as in biotechnology. Methods of producing the matrix are also provided. Mechanical and physical parameters can be controlled by the use of auxiliary components or additives which may be removed after the matrix is formed in order to improve the biological properties of the matrix. These matrices may be used clinically per se or as a cell-bearing implant.

    摘要翻译： 一种冷冻干燥的生物相容性基质，其包括可用作组织工程和生物技术植入物的血浆蛋白质。 还提供了制备基质的方法。 机械和物理参数可以通过使用辅助组分或添加剂来控制，辅助组分或添加剂可以在形成基质之后除去，以改善基质的生物学性质。 这些基质本身可以用作细胞携带植入物。

    Methods and compositions for treating respiratory mucin production associated disease conditions
    7.
    发明申请
    Methods and compositions for treating respiratory mucin production associated disease conditions 失效
    用于治疗呼吸道粘蛋白生成相关疾病状况的方法和组合物

    公开(公告)号：US20040203144A1

    公开(公告)日：2004-10-14

    申请号：US09861038

    申请日：2001-05-18

    发明人： David Edmund Szymkowski

    IPC分类号： C12N005/02 ,  A61K038/17

    CPC分类号： G01N33/5008 ,  A01K2217/05 ,  C07K14/47 ,  C07K14/4727 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/68 ,  G01N2333/4725

    摘要： Methods and compositions for modulating mucin secretion by respiratory system cells are provided. In the subject methods, an effective amount of an hCLCA2 (or homolog thereof) modulatory agent is contacted with the cell to modulate mucin secretion, e.g., decrease or increase mucin secretion, by the cell. The subject methods find use in a variety of different applications, including the treatment of disease conditions associated with respiratory system mucin secretion, e.g., mucin hyper- or hyposecretion. Also provided are methods of screening for respiratory system mucin secretion modulatory agents. Finally, mCLCA4 and non-human homologs thereof, as well as nucleic acid compositions encoding the same, are provided.

    摘要翻译： 提供了调节呼吸系统细胞粘蛋白分泌的方法和组合物。 在本方法中，有效量的hCLCA2（或其同系物）调节剂与细胞接触以调节细胞的粘蛋白分泌，例如降低或增加粘蛋白分泌。 主题方法可用于各种不同的应用，包括治疗与呼吸系统粘蛋白分泌相关的疾病状况，例如粘蛋白高或低分泌。 还提供了筛选呼吸系统粘蛋白分泌调节剂的方法。 最后，提供了mCLCA4及其非人类同系物，以及编码其的核酸组合物。