公开(公告)号：US20040268443A1

公开(公告)日：2004-12-30

申请号：US10834786

申请日：2004-04-29

Inventor： Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim

IPC: C12N015/82 ,  C12N015/87 ,  A01H005/00 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10 ,  A01H005/10

CPC classification number: C12N15/8261 ,  Y02A40/146

Abstract: An isolated nucleic acid construct including a nucleic acid molecule encoding a light-labile, phytochrome A, a light-inducible promoter which is 5null to the nucleic acid molecule encoding a light-labile, phytochrome A, and a terminator region which is 3null to the nucleic acid molecule encoding a light-labile, phytochrome A is disclosed. Methods for regulating a plant's canopy architecture and regulating a plant's seed yield, which involve transgenic plants or transgenic plant seeds including an isolated nucleic acid construct according to the present invention, are also disclosed.

公开(公告)号：US20040250309A1

公开(公告)日：2004-12-09

申请号：US10389432

申请日：2003-03-14

Applicant: Verdia, Inc.

Inventor： Mathias L. Muller ,  Thom True ,  Carl R. Simmons ,  Nasser Yalpani

IPC: C12N015/82 ,  C07H021/04 ,  C12N015/87 ,  A01H005/00 ,  C12N015/09 ,  C12N015/63 ,  C07K014/00 ,  C07K016/00 ,  C12N015/29

CPC classification number: C12Y302/01014 ,  C12N9/2442 ,  C12N15/8282 ,  C12N15/8286 ,  Y02A40/162

Abstract: The present invention provides compositions with chitinase activity and methods for using such compositions to enhance resistance of plants to fungal infections.

Abstract translation: 本发明提供具有几丁质酶活性的组合物和使用这些组合物增强植物对真菌感染的抗性的方法。

公开(公告)号：US20040248303A1

公开(公告)日：2004-12-09

申请号：US10760752

申请日：2004-01-20

Applicant: Syngenta Participations AG

Inventor： Rebecca M. Cade ,  Robert A. Dietrich

IPC: C12N015/82 ,  C07H021/04 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10

CPC classification number: C12N15/8281 ,  C07K14/415 ,  C12N15/8282

Abstract: The Arabidopsis NI16 gene was isolated in a yeast 2-hybrid screen via its interaction with the NIM1 protein and encodes a protein involved in the regulation of SAR gene expression in plants. NI16 is strongly induced in NIM1-overexpressing plants treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH). The nucleic acid sequence of the Arabidopsis NI16 promoter is disclosed herein.

Abstract translation: 通过与NIM1蛋白的相互作用，在酵母2-杂交筛选中分离拟南芥NI16基因，并编码参与植物中SAR基因表达调控的蛋白质。 NI16在用苯并（1,2,3）噻二唑-7-硫代硫酸S-甲酯（BTH）处理的NIM1过表达植物中强烈诱导。 本文公开了拟南芥NI16启动子的核酸序列。

公开(公告)号：US20040216185A1

公开(公告)日：2004-10-28

申请号：US10851894

申请日：2004-05-24

Inventor： Katayoon Dehesh ,  Dale Val

IPC: C12N015/82 ,  C12N015/87 ,  C12N005/04 ,  C12N015/63 ,  A01H005/00

CPC classification number: C12N9/1029 ,  C12N15/8247 ,  C12P7/6409 ,  C12P7/6463

Abstract: Methods of altering substrate specificity of beta-ketoacyl-ACP synthase, and engineered beta-ketoacyl-ACP synthases so produced are provided. DNA sequences and constructs for expression of engineered beta-ketoacyl-ACP synthases, as well as the novel beta-ketoacyl-ACP synthases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered beta-ketoacyl-ACP synthases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition.

Abstract translation: 提供了改变β-酮酰基-ACP合成酶底物特异性的方法和如此制备的工程化β-酮酰基-ACP合成酶。 还提供了用于表达工程化β-酮酸酰-ACP合酶的DNA序列和构建体，以及由其产生的新型β-酮酰基-ACP合成酶。 这样的DNA序列可用于在宿主细胞，特别是油菜作物植物的种子细胞中表达改造的β-酮酸酰-ACP合酶，用于改变脂肪酸组成。

公开(公告)号：US20040214318A1

公开(公告)日：2004-10-28

申请号：US10858775

申请日：2004-06-01

Inventor： Sean Chapman ,  William O. Dawson ,  Jonothan Donson ,  Monto H. Kumagai ,  Dennis J. Lewandowski ,  John A. Lindbo ,  Gregory P. Pogue ,  Shailaja Shivprasad

IPC: C07H021/04 ,  C12N015/09 ,  C12N015/63 ,  C12N005/04 ,  C12N015/87 ,  C12N015/00 ,  C12N015/70 ,  C12N015/85

CPC classification number: C12N9/2422 ,  A01H1/04 ,  A01H5/12 ,  C07K14/61 ,  C07K14/805 ,  C07K14/82 ,  C07K16/3061 ,  C07K2317/13 ,  C07K2317/622 ,  C12N15/1034 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8245 ,  C12N15/8257 ,  C12N15/8258 ,  C12Q1/68

Abstract: The present invention provides a method for enhancing the production of RNAs or proteins in a plant host using either non-native 5null untranslated sequences or artificial leader sequences. Preferably, commercially useful proteins, polypeptides, or fusion products thereof are produced, such as, enzymes, antibodies, hormones, pharmaceuticals, vaccines, pigments, anti-microbial polypeptides, and the like. The non-native 5null untranslated enhancers may also be effective in many different types of transcription or translation systems, such as bacterial and animal systems.

Abstract translation: 本发明提供了使用非天然5'非翻译序列或人造前导序列来增强植物宿主中RNA或蛋白质产生的方法。 优选地，产生商业上有用的蛋白质，多肽或其融合产物，例如酶，抗体，激素，药物，疫苗，颜料，抗微生物多肽等。 非天然的5'非翻译增强子也可以在许多不同类型的转录或翻译系统中有效，例如细菌和动物系统。

公开(公告)号：US20040210957A1

公开(公告)日：2004-10-21

申请号：US10656394

申请日：2003-09-05

Applicant: The Ohio State University

Inventor： Shaohong Qu ,  Guo-liang Wang ,  Bo Zhou

IPC: C12N015/29 ,  C07H021/04 ,  C12N015/82 ,  A01H005/00 ,  C12N015/87 ,  C12N015/63

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: Compositions and methods for enhancing or creating plant disease resistance to plant pests are provided. Transforming a plant with a novel rice Pi2-like disease resistance gene of the invention enhances disease resistance of the plant. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.

Abstract translation: 提供了用于增强或产生对植物害虫的植物病害抗性的组合物和方法。 用本发明的新型水稻Pi2样抗病基因转化植物提高了植物的抗病性。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

公开(公告)号：US20040210956A1

公开(公告)日：2004-10-21

申请号：US10479638

申请日：2004-05-05

Inventor： Don A. Roth ,  Randolph V. Lewis

IPC: C12N015/82 ,  C12N015/87 ,  C12N015/63 ,  C07K014/00 ,  C12N005/04

CPC classification number: C07K14/43518 ,  C12N15/8257

Abstract: The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.

Abstract translation: 本发明涉及分子生物学和植物生物学领域。 具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。

公开(公告)号：US20040208847A1

公开(公告)日：2004-10-21

申请号：US10400531

申请日：2003-03-28

Inventor： Fabienne Rolling ,  Michel Weber

IPC: A01N063/00 ,  A01N065/00 ,  A61K048/00 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74

CPC classification number: C12N15/86 ,  A61K48/0075 ,  C12N2750/14143

Abstract: A method for selectively transducing retinal pigment epithelium (RPE) cells in an eye of a mammal, comprises administering to the mammal a vector particle exhibiting an AAV-4 capsid protein.

Abstract translation: 用于在哺乳动物的眼睛中选择性转导视网膜色素上皮（RPE）细胞的方法包括向哺乳动物施用呈现AAV-4衣壳蛋白的载体颗粒。

公开(公告)号：US20040205841A1

公开(公告)日：2004-10-14

申请号：US10638464

申请日：2003-08-12

Inventor： Christopher Lawrence

IPC: C12N015/82 ,  C07H021/04 ,  A01H001/00 ,  C12N015/87 ,  C12N015/63

CPC classification number: C12N15/8282 ,  C12N15/8239

Abstract: Pathogen-inducible plant promoters are identified. The promoters control expression of nullhypersensitive responsenull proteins, including a protease inhibitor, an isocitrate lyase, and a glycoprotein. Heterologous gene sequences are produced by operably linking a promoter according to the present invention with a gene to be expressed in a transformed plant. Transformed plants are made by transforming a vector with a heterologous gene according to the present invention and then transforming the plant with the transformed vector. The transformed plants are capable of expressing a pre-selected protein in response to challenge by a plant pathogen, for example tobacco blue moldnullP. tabacina.

Abstract translation: 确定病原体诱导型植物启动子。 启动子控制“过敏反应”蛋白质的表达，包括蛋白酶抑制剂，异柠檬酸裂解酶和糖蛋白。 通过将根据本发明的启动子与在转化植物中表达的基因可操作地连接来产生异源基因序列。 通过用根据本发明的异源基因转化载体然后用转化的载体转化植物来制备转化的植物。 转化的植物能够响应于植物病原体（例如烟草蓝霉素-P）的攻击而表达预先选择的蛋白质。 tabacina

公开(公告)号：US20040194155A1

公开(公告)日：2004-09-30

申请号：US10678443

申请日：2003-10-01

Applicant: GILEAD SCIENCES, INC.

Inventor： William E. Delaney ,  Xiaofeng Xiong

IPC: A01K067/00 ,  A01K067/033 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  C12N005/00 ,  C12N005/02

CPC classification number: C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  C12N9/1276 ,  C12N2730/10122

Abstract: Applicants have identified 5 mutants associated with hepatitis B virus resistance to adefovir, a nucleotide analogue antiviral drug widely employed in the therapy of hepatitis B. In accord with this invention, reverse transcriptase mutants rtN236T, rtA181V, rtA181T and their corresponding surface antigen mutants sL173F and sL172trunc are provided. The mutant proteins, antibodies thereto and nucleic acids encoding the mutants have diagnostic value in monitoring and adjusting patient therapy with adefovir and in the therapy of patients infected with the mutants.

Abstract translation: 申请人已经确定了与乙型肝炎病毒抗性相关的5种突变体，阿德福韦，广泛用于乙型肝炎治疗的核苷酸类似物抗病毒药物。根据本发明，逆转录酶突变体rtN236T，rtA181V，rtA181T及其相应的表面抗原突变体sL173F和 提供sL172trunc。 突变蛋白，其抗体和编码突变体的核酸在监测和调整阿德福韦的患者治疗以及感染突变体的患者的治疗方面具有诊断价值。