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62 条记录 (耗时 0.019 秒)

    Precipitation of multivalent antiligands with affinity surfactants
    3.
    发明授权
    Precipitation of multivalent antiligands with affinity surfactants 失效
    用亲和表面活性剂沉淀多价抗配糖

    公开(公告)号：US5167925A

    公开(公告)日：1992-12-01

    申请号：US861471

    申请日：1992-04-01

    申请人： Ruben G. Carbonell ,  Roberto Guzman ,  Peter K. Kilpatrick

    发明人： Ruben G. Carbonell ,  Roberto Guzman ,  Peter K. Kilpatrick

    IPC分类号： G01N33/53 ,  G01N33/539

    CPC分类号： G01N33/53 ,  G01N33/539 ,  Y10S436/808

    摘要： Surfactants bound to a ligand and dissolved in a single phase aqueous solution form a precipitate when a multivalent antiligand is added to the solution. This invention can be used in an affinity precipitation test procedure (and kit) for detecting the presence or absence of a multivalent antiligand in a sample suspected of containing the multivalent antiligand, in an affinity precipitation inhibition test procedure (and kit) for detecting the presence or absence of a target ligand in a sample suspected of containing the target ligand, and in a process for separating a multivalent antiligand from a crude material containing the multivalent antiligand.

    摘要翻译： 当向溶液中加入多价抗配偶药物时，与配体结合并溶解在单相水溶液中的表面活性剂形成沉淀物。 本发明可用于亲和沉淀试验程序（和试剂盒），用于在亲和沉淀抑制试验程序（和试剂盒）中用于检测疑似含有多价反配体的样品中是否存在多价抗配偶体，以检测其存在 或在怀疑含有靶配体的样品中不存在目标配体，以及从含有多价抗配偶体的粗制物质中分离多价抗配偶体的方法。

    Immunoanalysis method for discriminating between antigen excess and antibody excess conditions
    5.
    发明授权
    Immunoanalysis method for discriminating between antigen excess and antibody excess conditions 失效
    用于区分抗原过量和抗体过量条件的免疫分析方法

    公开(公告)号：US5019999A

    公开(公告)日：1991-05-28

    申请号：US252420

    申请日：1988-09-30

    申请人： Chester Swirski, Jr.

    发明人： Chester Swirski, Jr.

    IPC分类号： G01N33/53 ,  G01N33/536 ,  G01N33/539 ,  G01N33/557

    CPC分类号： G01N33/557 ,  G01N33/539 ,  Y10S436/805

    摘要： A method of immunoassay for analyzing a bodily fluid for the presence of an analyte relies on observing substantially the entire course of a precipitin reaction from initiation to substantial completion of the precipitation, defining a mathematical function which represents the amount of precipitation versus time and contains constants which can be adjusted so that the function matches the observed precipitation versus time curve, and fitting the observed data to the mathematical function by adjusting the constants so that the mathematical function matches the observed precipitation versus time curve as closely as possible. Values for the maximum rate of precipitation and for the time interval from initiation of the precipitation to the time of maximum rate of precipitation are calculated from the mathematical function. A reference curve is generated using standard containing known concentrations of analyte according to conventional analytical procedures. The values so calculated for the parameters of the precipitation can then be used to determine whether a given sample presents a condition of antigen (analyte) excess and must be discarded or a condition of antibody excess which can be used in conjunction with the reference curve to report a valid concentration of analyte.

    Chlorpromazine assay
    6.
    发明授权
    Chlorpromazine assay 失效
    氯丙嗪测定

    公开(公告)号：US3976763A

    公开(公告)日：1976-08-24

    申请号：US573227

    申请日：1975-04-30

    申请人： Sidney Spector

    发明人： Sidney Spector

    IPC分类号： C07K16/44 ,  G01N33/531 ,  G01N33/539 ,  G01N33/00 ,  A61K39/00

    CPC分类号： G01N33/539 ,  C07K16/44 ,  G01N33/531 ,  Y10S436/804 ,  Y10S436/815 ,  Y10S436/823 ,  Y10S530/806

    摘要： A sensitive radioimmunoassay for chlorpromazine is described. To prepare the chlorpromazine selective antiserum, an antigen is made comprising chlorpromazine covalently bonded to an immunogenic carrier material through a diazo containing linking group and the antigen is injected into a suitable host mammal to elicit the desired antiserum. A preferred linking group is diazophenylcarbonyl while bovine serum albumin is a preferred immunogenic carrier material.

    摘要翻译： 描述了氯丙嗪的敏感放射免疫测定法。 为了制备氯丙嗪选择性抗血清，制备包含通过含重氮连接基团与免疫原性载体材料共价结合的氯丙嗪的抗原，并将抗原注射到合适的宿主哺乳动物中以引出所需的抗血清。 优选的连接基团是二氮苯基羰基，而牛血清白蛋白是优选的免疫原性载体材料。

    Trifunctional conjugates
    10.
    发明授权
    Trifunctional conjugates 失效
    三功能共轭物

    公开(公告)号：US5661019A

    公开(公告)日：1997-08-26

    申请号：US410014

    申请日：1995-03-22

    申请人： Chan S. Oh ,  James C. Sternberg

    发明人： Chan S. Oh ,  James C. Sternberg

    IPC分类号： C07K14/00 ,  C07K1/107 ,  C07K14/575 ,  C07K16/00 ,  C07K17/00 ,  G01N33/531 ,  G01N33/532 ,  G01N33/536 ,  G01N33/539 ,  G01N33/543 ,  G01N33/94 ,  C12N11/00 ,  C12N11/06 ,  G01N33/547

    CPC分类号： G01N33/54353 ,  G01N33/531 ,  G01N33/532 ,  G01N33/539 ,  G01N33/54313 ,  G01N33/94 ,  Y10S435/964 ,  Y10S436/819 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/816

    摘要： A trifunctional conjugate is providing having three chemical moieties attached through a spacer moiety. At least two of the chemical moieties are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected to impart certain steric properties to the conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the chemical moieties sterically inhibits the binding of a different macromolecule to another chemical moieties. In another embodiment, the binding of a first chemical moiety to a macromolecule restricts the subsequent binding of a second tridentate member to a proximate location on the same macromolecule. The three chemical moieties are preferably a nitrophenylazido residue, a phenyl boronic acid residue, and a solid support or a label such as biotin. The spacer is preferably cysteine, lysine, glutamic acid, pyroglutamic acid, S-acetylmercaptosuccinic anhydride or .omega.-carbobenzoxylysine. The conjugate is useful in immunoassays and for targeted labeling of proteins.

    摘要翻译： 提供具有通过间隔基部分连接的三个化学部分的三官能共轭物。 至少两个化学部分是相对小的分子，通常小于约7,000道尔顿。 选择间隔基部分以赋予缀合物一定的空间特性。 在一个实施方案中，大分子特异性结合配偶体与其中一个化学部分的结合空间地抑制不同大分子与另一化学部分的结合。 在另一个实施方案中，第一化学部分与大分子的结合限制了第二三齿构件随后与相同大分子上的邻近位置的结合。 三个化学部分优选为硝基苯基叠氮基残基，苯基硼酸残基和固体支持物或诸如生物素的标记。 间隔物优选为半胱氨酸，赖氨酸，谷氨酸，焦谷氨酸，S-乙酰巯基琥珀酸酐或ω-羧基苯甲氧基赖氨酸。 该缀合物可用于免疫测定和蛋白质靶向标记。