知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

20 records (took 0.049 seconds)

    Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
    1.
    发明授权
    Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays 有权

    公开(公告)号：US10786800B2

    公开(公告)日：2020-09-29

    申请号：US15477902

    申请日：2017-04-03

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: G01N21/05 ,  B01J19/00 ,  G01N35/00 ,  G01N21/64 ,  B01L3/00 ,  G01N33/543 ,  G01N21/03

    Abstract: A method and system for heating and/or inspecting a portable microfluidic assay cartridge for performing an assay includes receiving the assay cartridge on a receiving region of a translatable table under automated control, heating the cartridge, during performance of the assay, with a planar radiant heater plate, the heater plate having an aperture through which an inspection axis extends, and/or inspecting the cartridge using an optical system constructed to inspect the cartridge along the inspection axis by reading a fluorescent light signal which passes through the aperture in the heater plate. In addition, the cartridge moves with movement of the translation table, and the heater plate and optical system may be stationary, and the inspection axis may be fixed.

    Microfluidic devices and methods of manufacture and use
    3.
    发明授权
    Microfluidic devices and methods of manufacture and use 有权

    公开(公告)号：US10252263B2

    公开(公告)日：2019-04-09

    申请号：US14955785

    申请日：2015-12-01

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: B29C65/00 ,  B29C65/02 ,  B32B37/00 ,  B32B38/00 ,  B65C3/26 ,  B32B38/04 ,  B32B27/00 ,  G01N33/00 ,  G01N15/06 ,  G01N33/48 ,  B01L3/00 ,  C12M1/34 ,  C12M3/00 ,  F15C1/06 ,  G01N21/05 ,  G01N21/64 ,  G01N33/543 ,  B32B37/14 ,  B32B37/18 ,  G01N21/03

    Abstract: Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/−25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments. The microfluidic devices feature flow elements, channels, valves, and on-board pumps that are low cost to fabricate accurately, are minimally invasive to the fluid path and when implemented for the purpose, can produce multiplex assays on a single portable assay cartridge (chip) that have low coefficients of variation. Novel methods of construction, assembly and use of these features are presented, including co-valent bonding of selected regions of faces of surface-activatable bondable materials, such as PDMS to PDMS and PDMS to glass, while contiguous portions of one flexible sheet completes and seals flow channels, fixes the position of inserted analyte-detection elements in the channels, especially short hollow flow elements through which sample and reagent flow, and other portions form flexible valve membranes and diaphragms of pumps. A repeated make-and-break-contact manufacturing protocol prevents such bonding to interfere with moving the integral valve diaphragm portions from their valve seats defined by the opposed sheet member, which the flexible sheet material engages. Preparation of two subassemblies, each having a backing of relatively rigid material, followed by their assembly face-to-face in a permanent bond is shown. Hollow detection flow elements are shown fixed in channels, that provide by-pass flow paths of at least 50% of the flow capacity through the elements; in preferred implementations, as much as 100% or more. Metallized polyester film is shown to have numerous configurations and advantages in non-permanently bonded constructions. A method of preparing detection elements for an assay comprises batch coating detection elements, or hollow flow elements by mixing and picking and placing the elements in flow channels of a microfluidic device, capturing the flow elements by bonding two opposed layers while sealing the flow channels.

    MICROFLUIDIC DEVICES AND METHODS OF MANUFACTURE AND USE
    8.
    发明申请
    MICROFLUIDIC DEVICES AND METHODS OF MANUFACTURE AND USE 审中-公开

    公开(公告)号：US20160158750A1

    公开(公告)日：2016-06-09

    申请号：US14955785

    申请日：2015-12-01

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: B01L3/00 ,  G01N33/543

    CPC classification number: B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2300/168 ,  B01L2400/0481 ,  B01L2400/06 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/086 ,  B29C66/026 ,  B29C66/028 ,  B29C66/5225 ,  B29C66/52298 ,  B29C66/712 ,  B32B37/142 ,  B32B37/18 ,  B32B38/0008 ,  B32B2535/00 ,  G01N21/05 ,  G01N21/645 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2021/0346 ,  G01N2021/058 ,  Y10T29/494

    Abstract: Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/−25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments. The microfluidic devices feature flow elements, channels, valves, and on-board pumps that are low cost to fabricate accurately, are minimally invasive to the fluid path and when implemented for the purpose, can produce multiplex assays on a single portable assay cartridge (chip) that have low coefficients of variation. Novel methods of construction, assembly and use of these features are presented, including co-valent bonding of selected regions of faces of surface-activatable bondable materials, such as PDMS to PDMS and PDMS to glass, while contiguous portions of one flexible sheet completes and seals flow channels, fixes the position of inserted analyte-detection elements in the channels, especially short hollow flow elements through which sample and reagent flow, and other portions form flexible valve membranes and diaphragms of pumps. A repeated make-and-break-contact manufacturing protocol prevents such bonding to interfere with moving the integral valve diaphragm portions from their valve seats defined by the opposed sheet member, which the flexible sheet material engages. Preparation of two subassemblies, each having a backing of relatively rigid material, followed by their assembly face-to-face in a permanent bond is shown. Hollow detection flow elements are shown fixed in channels, that provide by-pass flow paths of at least 50% of the flow capacity through the elements; in preferred implementations, as much as 100% or more. Metallized polyester film is shown to have numerous configurations and advantages in non-permanently bonded constructions. A method of preparing detection elements for an assay comprises batch coating detection elements, or hollow flow elements by mixing and picking and placing the elements in flow channels of a microfluidic device, capturing the flow elements by bonding two opposed layers while sealing the flow channels.

    PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
    10.
    发明授权
    PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use 有权

    公开(公告)号：US10209250B2

    公开(公告)日：2019-02-19

    申请号：US15638526

    申请日：2017-06-30

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  John H. Leamon ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: G01N33/543 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G01N21/03 ,  G01N35/00

    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent. In some embodiments, the network may have at least two microfluidic channels, each channel of the two channels arranged to receive portions of the same fluid and to be fluidicly isolatable from each other, the device having at least two micro-particles disposed in fixed position in the network channels, the micro-particles being functionalized with a capture agent, one of the micro-particles in one of the channels being functionalized with an antibody or antigen capture agent and another of the micro-particles in another of the channels being functionalized with a nucleic acid capture agent. The method may also include detecting both protein and nucleic acid present in an input sample using the respectively functionalized micro particles. In some embodiments, the micro particles may be micro-length tubes or glass nano reactors.

Patent Agency Ranking