公开(公告)号：US20250033043A1

公开(公告)日：2025-01-30

申请号：US18733288

申请日：2024-06-04

Applicant: ProteinSimple

Inventor： Cheng ZHOU ,  Hui XU ,  Roland Y. WANG ,  David J. ROACH ,  Jessica DERMODY ,  Tom Weisan YANG ,  Jiaqi WU

IPC: B01L3/00

Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.

公开(公告)号：US20240344982A1

公开(公告)日：2024-10-17

申请号：US18604002

申请日：2024-03-13

Applicant: ProteinSimple

Inventor： James MCELROY ,  Christopher HEGER

IPC: G01N21/64 ,  G01N33/58

CPC classification number: G01N21/6428 ,  G01N33/582 ,  G01N2440/20 ,  G01N2500/00

Abstract: Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms. In some instances, the method further includes applying a reducing agent and quantifying total-thiol content in the sample.

公开(公告)号：US20240310326A1

公开(公告)日：2024-09-19

申请号：US18415025

申请日：2024-01-17

Applicant: ProteinSimple

Inventor： Hui XU ,  Uyen NGUYEN

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  G01N27/44791

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

公开(公告)号：US20230272003A1

公开(公告)日：2023-08-31

申请号：US18301858

申请日：2023-04-17

Applicant: ProteinSimple

Inventor： Hui XU ,  Kenneth SWARTZ

IPC: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/543 ,  G01N33/68 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/50 ,  G06V20/69

CPC classification number: C07K1/26 ,  G01N1/40 ,  G01N21/76 ,  G01N33/54366 ,  G01N33/6803 ,  C07K1/28 ,  G01N1/20 ,  G06V10/141 ,  G06V10/507 ,  G06V20/693 ,  G06V20/695 ,  G01N2001/4038 ,  G01N2021/1765

Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

公开(公告)号：US20210356427A1

公开(公告)日：2021-11-18

申请号：US17385553

申请日：2021-07-26

Applicant: ProteinSimple

Inventor： Hui XU ,  Uyen NGUYEN

IPC: G01N27/447

Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.

公开(公告)号：US20210231607A1

公开(公告)日：2021-07-29

申请号：US17063123

申请日：2020-10-05

Applicant: ProteinSimple

Inventor： David J. ROACH ,  Tom Weisan YANG ,  Hui XU

IPC: G01N27/447

Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

公开(公告)号：US09804079B2

公开(公告)日：2017-10-31

申请号：US13866164

申请日：2013-04-19

Applicant: ProteinSimple

Inventor： Jiaqi Wu

IPC: G01N21/25 ,  A61K47/48 ,  G01N21/31 ,  G01N33/68 ,  G01N21/00

CPC classification number: G01N21/25 ,  A61K47/68 ,  G01N21/31 ,  G01N33/6854 ,  G01N33/6857

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

公开(公告)号：US09377440B2

公开(公告)日：2016-06-28

申请号：US13573799

申请日：2012-10-03

Applicant: ProteinSimple

Inventor： Jiaqi Wu ,  Tiemin Huang ,  Arthur H. Watson

IPC: G01N27/447 ,  G01N1/40 ,  B01L3/00

CPC classification number: G01N27/44795 ,  B01L3/5027 ,  G01N1/40 ,  G01N27/44739 ,  G01N2001/4038

Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Abstract translation: 公开了用于精确选择和提取在微通道中执行的等电聚焦产生的聚焦区域中的选定分析物的装置和方法。 交叉通道微流体装置包括在交点处彼此流体连通的样品混合物引入和分离通道和提取通道。 提供了用于选择性地将cIEF之后的分离区域的图案移动到交点的装置，并且提供用于施加提取压力以将包含所选分析物的单个区域引导到提取通道中并随后从提取通道收集的装置。

公开(公告)号：US20130280815A1

公开(公告)日：2013-10-24

申请号：US13866164

申请日：2013-04-19

Applicant: PROTEINSIMPLE

Inventor： Jiaqi Wu

IPC: G01N21/25

CPC classification number: G01N21/25 ,  A61K47/68 ,  G01N21/31 ,  G01N33/6854 ,  G01N33/6857

Abstract: Provided herein are IEF focusing methods for determining the number of drug molecules present in at least one antibody-drug conjugate (ADC) species subpopulation. In one embodiment, the method comprises performing free solution isoelectric focusing on a sample comprising at least one ADC species, to obtain a focused sample. The absorbance of the sample at two different wavelengths is then measured, for example, over a range of pI values. Absorbance values at the two different wavelengths are compared at at least one corresponding pI value, where the at least one corresponding pI value is the pI of the ADC subpopulation. The number of drug molecules in the at least one ADC species subpopulation is then determined based on the comparison. The methods provided herein can also be used to determine the number of specific binding pair members bound to its target specific binding pair member.

Abstract translation: 本文提供了用于确定存在于至少一种抗体 - 药物偶联物（ADC）物种亚群中的药物分子数目的IEF聚焦方法。 在一个实施方案中，该方法包括对包含至少一种ADC种类的样品进行自由溶液等电聚焦，以获得聚焦样品。 然后测量样品在两个不同波长处的吸光度，例如在pI值的范围内。 在两个不同波长处的吸光度值在至少一个对应的pI值进行比较，其中至少一个对应的pI值是ADC亚群的pI。 然后基于比较来确定至少一个ADC种子亚群中的药物分子的数量。 本文提供的方法还可用于确定与其靶特异性结合对成员结合的特异性结合对成员的数目。

公开(公告)号：US20240192164A1

公开(公告)日：2024-06-13

申请号：US18356156

申请日：2023-07-20

Applicant: ProteinSimple

Inventor： Kevin TRAN ,  Jessica DERMODY ,  Joshua I. MOLHO ,  Surashree KULKARNI ,  Annegret BOGE

IPC: G01N27/447 ,  G01N1/40 ,  G01N21/64 ,  G01N33/68

CPC classification number: G01N27/44726 ,  G01N1/40 ,  G01N21/6486 ,  G01N33/6854 ,  G01N2001/4038 ,  G01N1/4044

Abstract: Some embodiments described herein relate to a method that includes wetting a microfluidic device with a sample containing biological cells. The chip can contain multiple wells. The sample can be allowed to “settle,” such that individual cells migrate into and/or are captured by various wells. Analytes from the cells can be electrophoretically separated. A voltage can be applied across the chip causing analytes to migrate from the wells and into the separation medium under electromotive force, where they can be separated based, for example, on their mobility. After separation, a reagent can be applied to the chip. The reagent can be configured to increase a probe's ability to bind to at least a subset of the analytes and facilitate detection and/or quantification of the analytes. The analytes can then be probed, for example, with an antibody.