公开(公告)号：US09377440B2

公开(公告)日：2016-06-28

申请号：US13573799

申请日：2012-10-03

Applicant: ProteinSimple

Inventor： Jiaqi Wu ,  Tiemin Huang ,  Arthur H. Watson

IPC: G01N27/447 ,  G01N1/40 ,  B01L3/00

CPC classification number: G01N27/44795 ,  B01L3/5027 ,  G01N1/40 ,  G01N27/44739 ,  G01N2001/4038

Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Abstract translation: 公开了用于精确选择和提取在微通道中执行的等电聚焦产生的聚焦区域中的选定分析物的装置和方法。 交叉通道微流体装置包括在交点处彼此流体连通的样品混合物引入和分离通道和提取通道。 提供了用于选择性地将cIEF之后的分离区域的图案移动到交点的装置，并且提供用于施加提取压力以将包含所选分析物的单个区域引导到提取通道中并随后从提取通道收集的装置。