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公开(公告)号：US10703774B2
公开(公告)日：2020-07-07
申请号：US15348699
申请日：2016-11-10
IPC分类号： C07K1/22 , B01D15/00 , C07K16/12 , C07K17/10 , B01J20/286 , C07K14/31 , C07K16/06 , B01J20/32
摘要： The invention relates to a method of isolating an immunoglobulin, comprising the steps of:a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support,b) contacting a liquid sample comprising an immunoglobulin with the separation matrix,c) washing said separation matrix with a washing liquid,d) eluting the immunoglobulin from the separation matrix with an elution liquid, ande) cleaning the separation matrix with a cleaning liquid,wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US10662261B2
公开(公告)日：2020-05-26
申请号：US15937428
申请日：2018-03-27
发明人： Mattias Algotsson , Lars C. Andersson , Eggert Brekkan , Lee Hedemyr , Jean-Luc Maloisel , Ronnie Palmgren
IPC分类号： C08B37/00 , B01J20/289 , B01J20/32 , C07C237/10 , C07C323/60 , B01J20/281
摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.
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公开(公告)号：US20190023735A1
公开(公告)日：2019-01-24
申请号：US16115756
申请日：2018-08-29
IPC分类号： C07K1/16 , B01J20/32 , C07K1/36 , B01J20/28 , B01J20/26 , B01D15/38 , G01N33/544 , B01J20/289 , B01J41/20 , B01D15/36 , B01D15/32
摘要： The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.
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公开(公告)号：US10124328B2
公开(公告)日：2018-11-13
申请号：US15407240
申请日：2017-01-16
IPC分类号： B01J39/26 , B01D15/18 , B01D15/36 , B01J20/286 , B01J20/32 , B01D15/38 , B01J20/26 , B01J20/28 , B01J20/289 , B01J20/30 , C07H1/08 , C07K1/22 , B01D15/34 , B01J20/24 , G01N30/88
摘要： The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
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公开(公告)号：US20180244729A1
公开(公告)日：2018-08-30
申请号：US15900419
申请日：2018-02-20
发明人： Gustav Rodrigo , Mats Ander , Tomas BJORKMAN , Goran Bauren
IPC分类号： C07K14/31 , B01D15/38 , C07K14/745 , C07K1/22 , C07K16/00 , B01J20/32 , B01J20/286
CPC分类号： C07K14/31 , B01D15/3809 , B01J20/286 , B01J20/3204 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/745 , C07K16/00
摘要： The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US09943781B2
公开(公告)日：2018-04-17
申请号：US13701251
申请日：2011-06-01
CPC分类号： B01D15/22 , B01D15/1864 , B01D15/1885 , F16L55/00 , G01N30/461 , G01N30/466 , G01N30/6004 , Y10T29/49826
摘要： A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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公开(公告)号：US20180043284A1
公开(公告)日：2018-02-15
申请号：US15793923
申请日：2017-10-25
发明人： KLAUS GEBAUER
CPC分类号： B01D15/14 , B01D15/1864 , B01D15/1885 , B01D15/22 , G01N30/466 , G01N30/6039 , G01N30/6043 , G01N30/6091 , G01N2030/8881 , Y10T29/49826
摘要： A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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公开(公告)号：US20170097327A1
公开(公告)日：2017-04-06
申请号：US15379447
申请日：2016-12-14
发明人： KLAUS GEBAUER
CPC分类号： G01N30/6004 , B01D15/18 , B01D15/22 , G01N30/6017 , G01N30/6021 , G01N30/6052 , G01N30/606
摘要： The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.
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公开(公告)号：US09573973B2
公开(公告)日：2017-02-21
申请号：US14396207
申请日：2013-04-22
IPC分类号： B01D15/36 , C07K1/22 , B01D15/18 , B01J20/286 , B01J20/32 , B01D15/38 , B01J20/26 , B01J20/28 , B01J20/289 , B01J20/30 , C07H1/08 , G01N30/88
CPC分类号： B01J39/26 , B01D15/1864 , B01D15/34 , B01D15/361 , B01D15/362 , B01D15/3809 , B01J20/24 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28014 , B01J20/28033 , B01J20/286 , B01J20/289 , B01J20/3085 , B01J20/3212 , B01J20/3278 , B01J20/3282 , B01J2220/80 , C07H1/08 , C07K1/22 , G01N2030/8827 , Y02P20/582
摘要： The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
摘要翻译： 本发明公开了一种从液体中的至少一种其它组分分离生物分子的方法，包括使所述液体与包含固体支持物和与所述固体支持物结合的聚合物链的分离基质接触的步骤。 聚合物链包含衍生自结构为CH 2 = CH-L-X的第一单体的单元，其中L为共价键或包含2-6个碳原子的烷基醚或羟基取代的烷基醚链，X为磺酸盐或膦酸酯基团。
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公开(公告)号：US09421479B2
公开(公告)日：2016-08-23
申请号：US13518509
申请日：2010-12-21
申请人： Klaus Gebauer
发明人： Klaus Gebauer
CPC分类号： B01D15/206 , B01D15/22 , G01N30/6017 , G01N30/6069 , G01N30/6082 , G01N30/6086
摘要： The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use.
摘要翻译： 本发明涉及用于色谱介质的容器或袋和使用这种容器的色谱柱的填充方法。 袋可用于储存和/或运输色谱介质，并且可以直接插入色谱柱的室中以供使用。
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