公开(公告)号：US20180135135A1

公开(公告)日：2018-05-17

申请号：US15811515

申请日：2017-11-13

Applicant: Quest Diagnostics Investments LLC

Inventor： Heather Sanders ,  Nigel J. Clarke

IPC: C12Q1/68 ,  G01N33/487 ,  C12N15/10 ,  G01N33/49 ,  G01N33/53 ,  G01N33/533

CPC classification number: C12Q1/6886 ,  C12N15/1058 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N33/4875 ,  G01N33/49 ,  G01N33/5308 ,  G01N33/533 ,  C12Q2527/125

Abstract: The present disclosure provides rapid and non-invasive methods for determining whether a patient exhibiting cancer symptoms, or at risk for hereditary cancers such as breast cancer, ovarian cancer, colon cancer, or skin cancer, will benefit from treatment with one or more therapeutic agents. These methods are based on detecting hereditary cancer-related mutations in small-volume dried biological fluid samples that are collected using a volumetric absorptive microsampling device (e.g., MITRA Tip). Kits for use in practicing the methods are also provided.

公开(公告)号：US20180134756A1

公开(公告)日：2018-05-17

申请号：US15706144

申请日：2017-09-15

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Takashi Sato ,  Yasunori Chiba ,  Hiroaki Tateno ,  Hiroyuki Kaji ,  Masanori Goto ,  Hisashi Narimatsu

IPC: C07K14/42 ,  G01N33/53

CPC classification number: C07K14/42 ,  G01N33/5308 ,  G01N2333/42 ,  G01N2400/00

Abstract: A Wisteria floribunda monomeric lectin polypeptide is provided. The Wisteria floribunda monomeric lectin polypeptide includes any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted, substituted, inserted, or added; and (3) the amino acid sequence defined in (1) or (2) above, further having an N-terminus deletion of one to 30 amino acids, in which Cys272 is alkylated, and the polypeptide is capable of specifically binding to a GalNAc terminal sugar chain.

公开(公告)号：US20180120326A1

公开(公告)日：2018-05-03

申请号：US15569619

申请日：2016-05-02

Applicant: IDEXX LABORATORIES, INC.

Inventor： Mahalakshmi Yerramilli ,  Edward Obare ,  John Joseph Quinn ,  Murthy VSN Yerramilli

IPC: G01N33/68 ,  G01N33/53 ,  C07C229/16 ,  C07K16/44

CPC classification number: G01N33/6812 ,  C07C229/16 ,  C07K16/44 ,  G01N33/5308 ,  G01N33/6806 ,  G01N2800/347

Abstract: Methods for determining renal function in an animal subject, the method including measuring the concentration of β-ammoisobutyric acid (β-amino isobutyrate) (BAIB) in patients samples and determining the presence, likelihood, or progression of kidney disease as a result of structural damage, or mortality associated with kidney disease. The methods also include measuring the concentration of BAIB in combination is symmetrical dimethyl arginine (SDMA) and determining kidney disease based upon the concentrations of BAIB and SDMA in the samples. Anti-BAIB antibodies, BAIB-conjugates, and assay methods using the antibodies and conjugates are also disclosed.

公开(公告)号：US20180119225A1

公开(公告)日：2018-05-03

申请号：US15440822

申请日：2017-02-23

Applicant: The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servic

Inventor： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00

Abstract: Provided herein are methods and kits for detecting 5-hydroxymethylated cytosine.

公开(公告)号：US20180119149A1

公开(公告)日：2018-05-03

申请号：US15797782

申请日：2017-10-30

Applicant: The Governors of the University of Alberta

Inventor： Zhixin WANG ,  Hongquan ZHANG ,  Xing-Fang LI ,  Xiaochun LE

IPC: C12N15/115 ,  G01N33/53 ,  G01N33/574

CPC classification number: C12N15/115 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2330/31 ,  G01N33/5308 ,  G01N33/57415 ,  G01N33/5748 ,  G01N2333/82

Abstract: The present disclosure relates generally to method of preparing aptamers, aptamers, and uses thereof.

公开(公告)号：US20180119113A1

公开(公告)日：2018-05-03

申请号：US15440815

申请日：2017-02-23

Applicant: The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Servi

Inventor： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC: C12N9/02 ,  G01N33/53 ,  G01N33/574 ,  C12N5/0783 ,  C12N15/873

CPC classification number: C12Q1/6827 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0696 ,  C12N9/0071 ,  C12N15/873 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2510/00 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/154 ,  G01N33/5011 ,  G01N33/5308 ,  G01N33/57426 ,  G01N33/57484 ,  G01N33/57496 ,  G01N2500/00 ,  C12Q2537/164 ,  C12Q2522/10 ,  C12Q2521/531

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US09952206B2

公开(公告)日：2018-04-24

申请号：US14184422

申请日：2014-02-19

Applicant: Randox Laboratories Limited

Inventor： Elouard Benchikh ,  Ivan McConnell ,  Philip Lowry ,  Peter Fitzgerald

IPC: G01N33/53 ,  C07K16/16 ,  C07K16/44

CPC classification number: G01N33/5308 ,  C07K16/16 ,  C07K16/44 ,  C07K2317/33

Abstract: The invention relates to the field of drug detection and describes antibody-based components methods and kits for the detection and quantification of alkaloids of the plant kratom. The invention is underpinned by novel antibodies which specifically bind to mitragynine alkaloids found in the kratom plant and their metabolites.

公开(公告)号：US09939443B2

公开(公告)日：2018-04-10

申请号：US14652728

申请日：2013-12-19

Applicant: CARIS SCIENCE, INC.

Inventor： David Spetzler ,  Valeriy Domenyuk ,  Tassilo Hornung ,  Günter Mayer ,  Michael Famulok

IPC: C12Q1/6886 ,  G01N33/574 ,  G01N33/50 ,  G01N33/53 ,  C12Q1/68 ,  C12N15/10

CPC classification number: G01N33/57488 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6886 ,  G01N33/5076 ,  G01N33/5308 ,  C12Q2525/205

Abstract: Methods are provided for selecting aptamers that are specific to a target of interest from amongst a library of potential aptamer sequences. Aptamers disclosed can be used to detect and/or characterize biological entities of interest, e.g. microvesicles and/or surface antigens. Further disclosed are biomarkers that can be used for diagnosing different disorders including different types of cancer.

公开(公告)号：US09939431B2

公开(公告)日：2018-04-10

申请号：US14227543

申请日：2014-03-27

Applicant: 6SensorLabs, Inc.

Inventor： Scott Sundvor ,  John Walton ,  Jonathan William Kiel ,  Jeffrey Mekler ,  Shireen Taleghani ,  Jacob Mooney

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/52 ,  G01N33/02 ,  G01N33/53 ,  G01N1/28

CPC classification number: G01N33/521 ,  G01N33/02 ,  G01N33/5308 ,  G01N2001/2866

Abstract: A self-contained apparatus and methods for detecting the presence of any specified substance in any medium. A sample of the medium is placed in a capsule, along with a solvent and a sensor configured to test for a target analyte. The solvent comes into contact with the medium in the capsule, and the capsule is agitated to create a dispersion in the solvent of a portion of any target analyte present in the medium. A release mechanism configured to cause conduction of the dispersion to the sensor, so that the sensor produces an indication of presence of the target analyte if the target analyte is present in the medium. The apparatus uses a disposable capsule where the medium in question is placed and the disposable capsule is placed inside a reader and analyzed for presence of the harmful substance.

公开(公告)号：US20180095078A1

公开(公告)日：2018-04-05

申请号：US15281077

申请日：2016-09-30

Applicant: Chin-Yih Hong ,  Herng-Er Horng

Inventor： Chin-Yih Hong ,  Herng-Er Horng

IPC: G01N33/543 ,  G01N33/53

CPC classification number: G01N33/54333 ,  C12N15/1013 ,  C12N15/1072 ,  C12Q1/6811 ,  C40B40/06 ,  G01N33/5308 ,  C12Q2525/205 ,  C12Q2563/143

Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.