专利检索 cpc:"C12N15/873" 第 1 页

1.

发明授权
Methods for making genetic edits 有权

公开(公告)号：US12070022B2

公开(公告)日：2024-08-27

申请号：US17379898

申请日：2021-07-19

申请人： Recombinetics, Inc.

发明人： Scott C. Fahrenkrug , Daniel F. Carlson

IPC分类号： C12N15/63 , A01K67/0275 , A01K67/0276 , C12N9/22 , C12N15/79 , C12N15/873 , C12N15/90

CPC分类号： A01K67/0276 , A01K67/0275 , C12N9/22 , C12N15/873 , C12N15/907 , A01K2217/07 , A01K2217/075 , A01K2217/15 , A01K2227/101 , A01K2227/108 , A01K2267/02

摘要： The present disclosure relates to methods for making genetic edits in vitro in a non-human vertebrate cell or embryo at a plurality of target chromosomal DNA sites. Methods for making a non-human animal having multiplex genetic edits at a plurality of target chromosomal DNA sites and making a non-human vertebrate animal chimeric for host cells and donor cells are also considered.

2.

发明授权
Device for collecting and culturing fish embryos and method for evaluating combined toxicity of thiamethoxam and tetraconazole 有权

公开(公告)号：US11969481B2

公开(公告)日：2024-04-30

申请号：US16944169

申请日：2020-07-31

申请人： Zhejiang Academy of Agricultural Sciences

发明人： Yanhua Wang , Shanshan Di , Guiling Yang , Zhiheng Zhang , Xinquan Wang , Qiang Wang

IPC分类号： C12M1/34 , A01K61/10 , A61D1/10 , A61K49/00 , C12M1/12 , C12M3/00 , C12N5/073 , C12N15/873 , C12Q1/20 , C12Q1/24

CPC分类号： A61K49/0008 , A01K61/10 , A61D1/10 , C12M21/08 , C12M25/02 , C12N5/0604 , C12N15/873 , Y02A40/81

摘要： A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.

3.

发明申请
METHOD OF PRODUCING INFERTILE FISH AND EGG-PRODUCING AQUATIC ANIMALS AND OF DELIVERING COMPOUNDS INTO EGGS AND EMBRYOS 审中-公开

公开(公告)号：US20180288984A1

公开(公告)日：2018-10-11

申请号：US16011631

申请日：2018-06-18

申请人： University of Maryland Baltimore County

发明人： Yonathan Zohar , Ten-Tsao Wong

IPC分类号： A01K67/027 , C12N15/113 , C12N15/873

CPC分类号： A01K67/0276 , A01K2207/05 , A01K2217/075 , A01K2227/40 , A01K2267/02 , C12N15/113 , C12N15/873 , C12N2310/11 , C12N2310/3233 , C12N2310/351 , Y02A90/40

摘要： Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.

4.

发明申请
MEASURING A LEVEL OF A 5-HYDROXYMETHYLCYTOSINE IN A SAMPLE FROM A SUBJECT HAVING A CANCER OR SUSPECTED OF HAVING CANCER 审中-公开

公开(公告)号：US20180224434A9

公开(公告)日：2018-08-09

申请号：US15440826

申请日：2017-02-23

申请人： The Children's Medical Center Corporation , The United States of America, As Represented by the Secretary, Department of Health & Human Servic

发明人： Anjana Rao , Mamta Tahiliani , Kian Peng Koh , Suneet Agarwal , Aravind Iyer

IPC分类号： G01N33/53 , G01N33/574 , C12N9/02

CPC分类号： C12Q1/6827 , C12N5/0018 , C12N5/0607 , C12N5/0637 , C12N5/0696 , C12N9/0071 , C12N15/873 , C12N2501/15 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/70 , C12N2501/71 , C12N2501/999 , C12N2506/1307 , C12N2506/1353 , C12N2510/00 , C12Q1/26 , C12Q1/6806 , C12Q1/6869 , C12Q1/6886 , C12Q2600/154 , G01N33/5011 , G01N33/5308 , G01N33/57426 , G01N33/57484 , G01N33/57496 , G01N2500/00 , C12Q2537/164 , C12Q2522/10 , C12Q2521/531

摘要： Provided herein are methods and kits for measuring a level of a 5-hydroxymethylcytosine in a nucleotide sequence from a subject, wherein the subject is a subject having a cancer or suspected of having cancer.

5.

发明申请
SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS 审中-公开

公开(公告)号：US20180044633A1

公开(公告)日：2018-02-15

申请号：US15722202

申请日：2017-10-02

申请人： The Children's Medical Center Corporation , The United States of America, As Represented by the Secretary, Department of Health & Human Servic

发明人： Anjana Rao , Mamta Tahiliani , Kian Peng Koh , Suneet Agarwal , Aravind Iyer

IPC分类号： C12N5/0783

CPC分类号： C12Q1/6827 , C12N5/0018 , C12N5/0607 , C12N5/0637 , C12N5/0696 , C12N9/0071 , C12N15/873 , C12N2501/15 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/70 , C12N2501/71 , C12N2501/999 , C12N2506/1307 , C12N2506/1353 , C12N2510/00 , C12Q1/26 , C12Q1/6806 , C12Q1/6869 , C12Q1/6886 , C12Q2521/531 , C12Q2522/10 , C12Q2537/164 , C12Q2600/154 , G01N33/5011 , G01N33/5308 , G01N33/57426 , G01N33/57484 , G01N33/57496 , G01N2500/00

摘要： The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

6.

发明授权
Production of fertile XY female mice from XY mouse ES cells 有权

公开(公告)号：US09885058B2

公开(公告)日：2018-02-06

申请号：US15486210

申请日：2017-04-12

申请人： REGENERON PHARMACEUTICALS, INC.

发明人： Wojtek Auerbach , Thomas DeChiara , William Poueymirou , David Frendewey , David Valenzuela

IPC分类号： C12N5/073 , A01K67/027 , C12N15/873

CPC分类号： A01K67/0276 , A01K67/0278 , A01K2207/12 , A01K2217/052 , A01K2217/054 , A01K2217/075 , A01K2227/105 , A01K2267/0331 , C12N5/0603 , C12N5/0606 , C12N9/0091 , C12N15/873 , C12N2500/34 , C12N2500/60 , C12N2517/02 , C12N2517/10

摘要： Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.

7.

发明申请
GENE KNOCKOUT METHOD 审中-公开

公开(公告)号：US20180020646A1

公开(公告)日：2018-01-25

申请号：US15538554

申请日：2015-12-25

申请人： RIKEN

发明人： Hiroki UEDA , Genshiro SUNAGAWA , Kenta SUMIYAMA , Maki UKAI , Dimitri PERRIN

IPC分类号： A01K67/027 , C12N15/11 , C12N15/87 , C12N15/873

CPC分类号： A01K67/0276 , A01K67/027 , A01K2207/05 , A01K2217/075 , C12N15/11 , C12N15/87 , C12N15/873 , C12N2310/20

摘要： A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.

8.

发明申请
METHOD FOR RECONSTRUCTING A NON-HUMAN ANIMAL EMBRYO 审中-公开

公开(公告)号：US20170204433A1

公开(公告)日：2017-07-20

申请号：US15304964

申请日：2015-04-22

申请人： UNIVERSITÀ DEGLI STUDI DI TERAMO

发明人： Pasqualino LOI , Domenico IUSO , Marta CZERNIK , Federica ZACCHINI , Grazyna PTAK , Saadi KHOCHBIN , Antonella FIDANZA

IPC分类号： C12N15/873 , A01K67/027 , C12N15/877

CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/00 , A01K2227/103 , C12N15/877 , C12N15/8773

摘要： The present invention refers to a method for reconstructing a non-human animal embryo, to a method for generating a nonhuman animal and to a non-human animal or non-human animal reconstructed embryo obtainable by the methods according to the invention. The method of somatic cell nuclear transfer (SCNT) has a step of transiently expressing or inducing the uptake of a protein involved in male sperm maturation, such as protamine or transition protein, in the somatic donor cell before nuclear transfer.