公开(公告)号：US20160266016A1

公开(公告)日：2016-09-15

申请号：US14911778

申请日：2014-08-05

申请人： RIKEN

发明人： Etsuo SUSAKI ,  Hiroki UEDA ,  Kazuki TAINAKA

IPC分类号： G01N1/30

CPC分类号： G01N1/30

摘要： The present invention provides an aminoalcohol together with, as necessary, at least one selected from the group consisting of: at least one compound selected from the group consisting of urea and a urea derivative; a nonionic detergent; and sucrose to provide a biological material having an excellent light-transmitting property. The present invention provides a technique that allows a high-throughput observation of gene expression/localization or cell morphology in tissue at an whole-tissue level.

摘要翻译： 本发明提供了必要时的氨基醇以及选自尿素和脲衍生物中的至少一种化合物中的至少一种。 非离子洗涤剂; 和蔗糖，以提供具有优异的透光性的生物材料。 本发明提供一种技术，其允许在全组织水平的组织中高通量观察基因表达/定位或细胞形态。

公开(公告)号：US20220381657A1

公开(公告)日：2022-12-01

申请号：US17868721

申请日：2022-07-19

申请人： RIKEN

发明人： Hiroki UEDA ,  Kazuki TAINAKA ,  Tatsuya MURAKAMI

IPC分类号： G01N1/30 ,  G01N33/48 ,  G01N1/28 ,  G01N33/483

摘要： The present invention relates to a clearing technique which uses a solution containing at least one of a compound having a delipidation ability, a compound having a biochrome decoloring ability, a compound having a decalcification ability, a compound having a refractive index adjusting ability, and a compound having a tissue swelling ability. The clearing technique is suitable for use in high-throughput and low-magnification imaging which involves a simple process.

公开(公告)号：US20190086302A1

公开(公告)日：2019-03-21

申请号：US16096130

申请日：2017-04-25

申请人： RIKEN

发明人： Hiroki UEDA ,  Kazuki TAINAKA ,  Tatsuya MURAKAMI

IPC分类号： G01N1/30 ,  G01N33/483

摘要： The present invention relates to a clearing technique which uses a solution containing at least one of a compound having a delipidation ability, a compound having a biochrome decoloring ability, a compound having a decalcification ability, a compound having a refractive index adjusting ability, and a compound having a tissue swelling ability. The clearing technique is suitable for use in high-throughput and low-magnification imaging which involves a simple process.

公开(公告)号：US20180020646A1

公开(公告)日：2018-01-25

申请号：US15538554

申请日：2015-12-25

申请人： RIKEN

发明人： Hiroki UEDA ,  Genshiro SUNAGAWA ,  Kenta SUMIYAMA ,  Maki UKAI ,  Dimitri PERRIN

IPC分类号： A01K67/027 ,  C12N15/11 ,  C12N15/87 ,  C12N15/873

CPC分类号： A01K67/0276 ,  A01K67/027 ,  A01K2207/05 ,  A01K2217/075 ,  C12N15/11 ,  C12N15/87 ,  C12N15/873 ,  C12N2310/20

摘要： A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.

公开(公告)号：US20140010735A1

公开(公告)日：2014-01-09

申请号：US13933160

申请日：2013-07-02

申请人： RIKEN

发明人： Yo TANAKA ,  Hiroki UEDA

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/569 ,  B01L2200/082 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2400/0481 ,  F04B43/021 ,  F04B43/046 ,  F16K99/0015 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094

摘要： The present invention relates to a fluid-controlling device (10) for incorporation into a microchip (100). The fluid-controlling device (10) of the present invention includes: a glass substrate (1b) having a hollow part (3) provided therein, the hollow part (3) having a first opening (3′) extending to a top surface of the glass substrate (1b) and a second opening (4) extending to a bottom surface of the glass substrate (1b); and a glass sheet (2) being 1 to 50 μm in thickness and sealing the first opening (3′), the glass sheet (2) changing a volume of the hollow part (3) when a position of the glass sheet (2) is displaced by and according to a pressing force applied toward the hollow part (3). With use of the present invention, it is possible to provide a versatile microchip system being usable under a high pressure and being usable with an organic solvent.

摘要翻译： 本发明涉及一种用于结合到微芯片（100）中的流体控制装置（10）。 本发明的流体控制装置（10）包括：具有设置在其中的中空部（3）的玻璃基板（1b），所述中空部（3）具有向第一开口（3'）延伸的第一开口 玻璃基板（1b）和延伸到玻璃基板（1b）的底面的第二开口（4）; 玻璃板（2）的厚度为1〜50μm的玻璃板（2），密封第1开口（3'），玻璃板（2）在玻璃板（2）的位置变更中空部（3） 被按照向中空部（3）施加的按压力移位。 利用本发明，可以提供一种可在高压下使用并且可用于有机溶剂的通用微芯片系统。