公开(公告)号：US20170204433A1

公开(公告)日：2017-07-20

申请号：US15304964

申请日：2015-04-22

申请人： UNIVERSITÀ DEGLI STUDI DI TERAMO

发明人： Pasqualino LOI ,  Domenico IUSO ,  Marta CZERNIK ,  Federica ZACCHINI ,  Grazyna PTAK ,  Saadi KHOCHBIN ,  Antonella FIDANZA

IPC分类号： C12N15/873 ,  A01K67/027 ,  C12N15/877

CPC分类号： C12N15/873 ,  A01K67/0273 ,  A01K2217/00 ,  A01K2227/103 ,  C12N15/877 ,  C12N15/8773

摘要： The present invention refers to a method for reconstructing a non-human animal embryo, to a method for generating a nonhuman animal and to a non-human animal or non-human animal reconstructed embryo obtainable by the methods according to the invention. The method of somatic cell nuclear transfer (SCNT) has a step of transiently expressing or inducing the uptake of a protein involved in male sperm maturation, such as protamine or transition protein, in the somatic donor cell before nuclear transfer.