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    COMPOSITIONS AND METHODS RELATING TO NON-HUMAN ANIMALS MODIFIED TO PROMOTE PRODUCTION OF SELECTED GAMETES
    1.
    发明申请
    COMPOSITIONS AND METHODS RELATING TO NON-HUMAN ANIMALS MODIFIED TO PROMOTE PRODUCTION OF SELECTED GAMETES 审中-公开
    与促进选择游戏生产有关的非人类动物相关的组合物和方法

    公开(公告)号：US20130298269A1

    公开(公告)日：2013-11-07

    申请号：US13941014

    申请日：2013-07-12

    申请人： The Jackson Laboratory

    发明人： Michael V. Wiles ,  Robert Taft

    IPC分类号： A01K67/02

    CPC分类号： A01K67/02 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2217/058 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/10 ,  A01K2267/02 ,  C12N15/873 ,  C12N15/877 ,  C12N15/8775

    摘要： Methods and compositions for producing selected non-human mammalian germ cells and gametes and for making non-human animals using the produced germ cells and gametes are provided by the present invention. Methods of generating a non-human embryo and/or animal derived from donor stem cells, methods of generating chimeric non-human animals having substantially all gametes and/or germ cells derived from the donor stem cells, methods of producing a non-human host embryo lacking functional endogenous germ cells and non-human host embryos incapable of developing endogenous gametes of the present invention are described herein.

    摘要翻译： 通过本发明提供用于产生选择的非人哺乳动物生殖细胞和配子以及使用所产生的生殖细胞和配子制备非人动物的方法和组合物。 产生源自供体干细胞的非人胚胎和/或动物的方法，产生具有基本上所有来自供体干细胞的配子和/或生殖细胞的嵌合非人动物的方法，生产非人宿主的方法 本文描述了缺乏功能性内源性生殖细胞的胚胎和不能发育本发明的内源性配子的非人宿主胚胎。

    METHODS FOR IMPROVEMENT OF BIRTH RATES IN CANIDAE ON SOMATIC CELL NUCLEAR TRANSFER
    2.
    发明申请
    METHODS FOR IMPROVEMENT OF BIRTH RATES IN CANIDAE ON SOMATIC CELL NUCLEAR TRANSFER 审中-公开
    改善角膜细胞核转移中生殖系统发生率的方法

    公开(公告)号：US20100293626A1

    公开(公告)日：2010-11-18

    申请号：US12517567

    申请日：2008-11-19

    申请人： Byeong Chun Lee ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  So Gun Hong ,  Jung Eun Park ,  Jung Taek Kang

    发明人： Byeong Chun Lee ,  Min Kyu Kim ,  Goo Jang ,  Hyun Ju Oh ,  So Gun Hong ,  Jung Eun Park ,  Jung Taek Kang

    IPC分类号： C12N15/06

    CPC分类号： C12N5/16 ,  A01K67/0273 ,  A01K2227/10 ,  C12N15/877 ,  C12N2501/405 ,  C12N2517/10

    摘要： The present invention relates to a method for increasing the efficiency of offspring production in producing animals belonging to the family Canidae (canines) by somatic cell nuclear transfer. More specifically, relates to a method for increasing the efficiency of production of cloned canines by a method for cloning canines comprising enucleating the oocyte of a canine to prepare an enucleated oocyte, fusing a nuclear donor cell with the enucleated oocyte to prepare a nuclear transfer embryo and transferring the nuclear transfer embryo into the oviduct of a surrogate mother, wherein the nuclear donor cell is cultured in a medium containing a specific cell cycle synchronization-inducing substance such as roscovitine in the preparation thereof. The method enables to clone canines with high efficiency, and thus can contribute to the development of studies in the fields of veterinary medicine, anthropology and medical science such as the propagation of superior canines, the conservation of rare or nearly extinct canines, xenotransplantation and disease animal models.

    摘要翻译： 本发明涉及通过体细胞核转移提高属于犬科（犬科）的动物的后代生产效率的方法。 更具体地，涉及通过克隆犬的方法来提高克隆犬的生产效率的方法，包括将犬的卵母细胞去核以制备去核卵母细胞，将核供体细胞与去核卵母细胞融合以制备核转移胚 并将核转移胚胎转移到替代母体的输卵管中，其中在其制备中，在含有特定细胞周期同步诱导物质如roscovitine的培养基中培养核供体细胞。 该方法能够高效地克隆犬科动物，从而有助于兽医，人类学和医学领域的研究发展，如高等犬传播，保护罕见或几乎灭绝的犬，异种移植和疾病 动物模型。

    Methods for correcting mitotic spindle defects and optimizing preimplantation embryonic developmental rates associated with somatic cell nuclear transfer in animals
    5.
    发明申请
    Methods for correcting mitotic spindle defects and optimizing preimplantation embryonic developmental rates associated with somatic cell nuclear transfer in animals 审中-公开
    纠正有丝分裂纺锤体缺陷和优化与动物体细胞核转移相关的植入前胚胎发育率的方法

    公开(公告)号：US20060037086A1

    公开(公告)日：2006-02-16

    申请号：US11003006

    申请日：2004-12-03

    申请人： Gerald Schatten ,  Calvin Simerly ,  Christopher Navara

    发明人： Gerald Schatten ,  Calvin Simerly ,  Christopher Navara

    IPC分类号： A01K67/00

    CPC分类号： A01K67/0273 ,  A01K2227/106 ,  C12N5/0604 ,  C12N5/0609 ,  C12N15/877 ,  C12N15/8776 ,  C12N2517/04

    摘要： The present invention is directed to various methodologies to make NT a practical procedure for animals, specifically, primates including human and non-human primates. Furthermore, the methods and molecular components provided by the present invention provide a practical means for producing embryos with desired characteristics. In a specific embodiment, the methodology of the present invention comprises introducing nuclei having desired characteristics along with one or more molecular components into an enucleated egg, thus creating a nuclear transfer construct, culturing the egg to produce a viable embryo, transferring the embryo to the oviducts of a female, and producing a cloned animal.

    摘要翻译： 本发明涉及使NT成为动物，特别是包括人和非人灵长类动物的灵长类动物的实用程序的各种方法。 此外，本发明提供的方法和分子组分提供了生产具有所需特征的胚胎的实用手段。 在具体实施方案中，本发明的方法包括将具有所需特征的核以及一种或多种分子成分引入去核卵中，从而产生核转移构建体，培养卵以产生活的胚胎，将胚胎转移至 雌性输卵管，并产生克隆的动物。

    Method for generating cloned animals using chromosome shuffling
    6.
    发明申请
    Method for generating cloned animals using chromosome shuffling 审中-公开
    使用染色体洗牌产生克隆动物的方法

    公开(公告)号：US20040139489A1

    公开(公告)日：2004-07-15

    申请号：US10398614

    申请日：2003-08-08

    发明人： Robert Lanza ,  Jose Cibelli ,  Philip Damiani ,  Michael D West

    IPC分类号： C12N015/00 ,  A01K067/027

    CPC分类号： C12N15/877 ,  A61K48/00

    摘要： The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with nullautosomally isogenicnull meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both nullautosomallynull and nullallelicallynull isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.

    摘要翻译： 本发明涉及在产生克隆和转基因动物的背景下使用染色体替代技术，以便校正染色体异常或改变常染色体基因型，并通过替代待克隆的动物中的性染色体来提供新的育种对。 性染色体或X或Y染色体的替代将导致具有“常染色体同源性”的常染色体同源异体和性别非同基因（AISN）的动物，这意味着每组中的成对的常染色体组（非性染色体） 动物是同基因的或相同的。 本发明还包括既“常染色体”和“等位性”同位素的动物，由此每个特定的一对染色体在单一动物内部以及动物之间是内生同源的或相同的。 这样的动物特别可用于使用有性繁殖产生一系列克隆的哺乳动物，而不必经受核转移以繁殖克隆的动物。

    Cloned non-human mammals from contact inhibited donor cells
    7.
    发明申请
    Cloned non-human mammals from contact inhibited donor cells 审中-公开
    从接触中克隆的非人哺乳动物抑制供体细胞

    公开(公告)号：US20030101469A1

    公开(公告)日：2003-05-29

    申请号：US10192025

    申请日：2002-07-09

    发明人： Kenneth Bondioli ,  Jaqdeece Ramsoondar ,  William Fodor

    IPC分类号： A01K067/027

    CPC分类号： C12N15/873 ,  C12N15/877

    摘要： Cloned non-human mammalian nuclear transfer units, embryos and animals are provided by introducing a contact-inhibited non-cycling mammalian donor cell in the G1 state or a nucleus thereof into an enucleated oocyte from a non-human mammal of the same species as the donor to form a nuclear transfer unit, implanting the nuclear transfer unit into the uterus of a surrogate mother, permitting the nuclear transfer unit to develop into the cloned non-human mammalian embryo and allowing the embryo to come to term.

    摘要翻译： 克隆的非人哺乳动物核转移单位，胚胎和动物通过将G1状态或其核中的接触抑制的非循环哺乳动物供体细胞引入来自与来自相同种类的非人哺乳动物的去核卵母细胞 供体形成核转移单元，将核转移单元植入替代母亲的子宫，允许核转移单位发育成克隆的非人哺乳动物胚胎并允许胚胎进入期限。

    Embryonic or stem-like cell lines produced by cross species nuclear transplantation
    8.
    发明申请
    Embryonic or stem-like cell lines produced by cross species nuclear transplantation 审中-公开
    通过异种核移植产生的胚胎或干细胞系

    公开(公告)号：US20010012513A1

    公开(公告)日：2001-08-09

    申请号：US09809018

    申请日：2001-03-16

    申请人： University of Massachusetts

    发明人： James Robl ,  Jose Cibelli ,  Steven L. Stice

    IPC分类号： A61K048/00 ,  C12N005/08

    CPC分类号： C12N15/873 ,  A01K2217/075 ,  A61K35/12 ,  A61K38/00 ,  C12N5/0603 ,  C12N15/87 ,  C12N15/877 ,  C12N2510/00 ,  C12N2517/04

    摘要： An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation.

    摘要翻译： 提供了涉及将供体细胞核移植到与供体细胞不同的物种的去核卵母细胞中的改进的核转移方法。 所得到的核转移单位可用于生产等原胚胎干细胞，特别是人原代胚胎干细胞。 这些胚胎或干细胞样细胞可用于产生所需的分化细胞并用于通过同源重组引入，去除或修饰所需基因，例如在这些细胞的基因组的特定位点。 这些可能含有异源基因的细胞特别适用于细胞移植治疗和体外研究细胞分化。

    Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
    10.
    发明授权
    Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions 有权
    通过交叉种核移植产生的胚胎或干细胞系以及通过供体细胞遗传改变或通过组织培养条件增强胚胎发育的方法

    公开(公告)号：US07696404B2

    公开(公告)日：2010-04-13

    申请号：US10329979

    申请日：2002-12-27

    申请人： Jose Cibelli ,  Michael D. West

    发明人： Jose Cibelli ,  Michael D. West

    IPC分类号： C12N15/00 ,  C12N5/02

    CPC分类号： C12N15/873 ,  A01K2217/075 ,  A61K35/12 ,  A61K38/00 ,  C12N15/87 ,  C12N15/877 ,  C12N2510/00 ,  C12N2517/04

    摘要： An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.

    摘要翻译： 提供了涉及将不同供体细胞核移植到与供体细胞不同的物种的去核卵母细胞中的改进的核转移方法。 所得到的核转移单位可用于生产等原胚胎干细胞，特别是人原代胚胎干细胞。 这些胚胎或干细胞样细胞可用于产生所需的分化细胞并用于通过同源重组引入，去除或修饰所需基因，例如在这些细胞的基因组的特定位点。 这些可能含有异源基因的细胞特别适用于细胞移植治疗和体外研究细胞分化。 此外，提供了通过遗传改变供体细胞以抑制细胞凋亡，选择特定细胞周期和/或增强胚胎生长和发育来提高核转移效率的方法。