公开(公告)号：US11969481B2

公开(公告)日：2024-04-30

申请号：US16944169

申请日：2020-07-31

申请人： Zhejiang Academy of Agricultural Sciences

发明人： Yanhua Wang ,  Shanshan Di ,  Guiling Yang ,  Zhiheng Zhang ,  Xinquan Wang ,  Qiang Wang

IPC分类号： C12M1/34 ,  A01K61/10 ,  A61D1/10 ,  A61K49/00 ,  C12M1/12 ,  C12M3/00 ,  C12N5/073 ,  C12N15/873 ,  C12Q1/20 ,  C12Q1/24

CPC分类号： A61K49/0008 ,  A01K61/10 ,  A61D1/10 ,  C12M21/08 ,  C12M25/02 ,  C12N5/0604 ,  C12N15/873 ,  Y02A40/81

摘要： A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.