cMET protein SRM/MRM assay
    10.
    发明授权
    cMET protein SRM/MRM assay 有权
    cMET蛋白SRM / MRM测定

    公开(公告)号:US09372195B2

    公开(公告)日:2016-06-21

    申请号:US13976956

    申请日:2011-12-27

    摘要: Specific peptides are provided, and derived ionization characteristics of those peptides, from the Hepatocyte Growth Factor Receptor (cMET) protein. The peptides are particularly and surprisingly advantageous for quantifying by the method of Selected Reaction Monitoring (SRM) mass spectrometry the cMET protein directly in biological samples that have been fixed in formalin, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed where the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including: formalin-fixed tissue/cells; formalin-fixed/paraffin embedded (FFPE) tissue/cells; FFPE tissue blocks and cells from those blocks; and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from the biological sample using the Liquid Tissue™ reagents and protocol and the cMET protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a cMET peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.

    摘要翻译: 提供特异性肽,并从肝细胞生长因子受体(cMET)蛋白提供这些肽的衍生电离特征。 通过选择反应监测(SRM)质谱法直接在已经在福尔马林中固定的生物样品中的cMET蛋白进行定量,或者也可以称为多反应监测(MRM)质谱法,这些肽特别令人惊奇地有利 。 这种生物样品被化学保存和固定,其中生物样品选自用含甲醛的试剂/固定剂处理的组织和细胞,包括:福尔马林固定的组织/细胞; 福尔马林固定/石蜡包埋(FFPE)组织/细胞; FFPE组织块和来自这些区块的细胞; 和已被福尔马林固定和石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从生物样品制备蛋白质样品,并且通过SRM / MRM质谱法的方法在液体组织样品中定量cMET蛋白,通过在蛋白质样品中定量至少一种或多种 描述的肽。 如果它们以修饰或未修饰的形式存在,则可以定量这些肽。 cMET肽的修饰形式的实例是肽序列内的酪氨酸,苏氨酸,丝氨酸和/或其他氨基酸残基的磷酸化。