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    Enrichment of DNA Sequencing Libraries from Samples Containing Small Amounts of Target DNA
    65.
    发明申请
    Enrichment of DNA Sequencing Libraries from Samples Containing Small Amounts of Target DNA 审中-公开
    从含有少量靶DNA的样品中富集DNA测序文库

    公开(公告)号：US20150360194A1

    公开(公告)日：2015-12-17

    申请号：US14653748

    申请日：2014-05-02

    Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

    Inventor： Carlos D. Bustamante ,  Meredith L. Carpenter ,  Jason D. Buenrostro ,  William J. Greenleaf

    IPC: B01J19/00 ,  C12Q1/68

    CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1006 ,  C12N15/1034 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q2525/143 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2563/131

    Abstract: Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted DNA; ligating universal adaptors to the extracted DNA; hybridizing the extracted DNA, in solution, with affinity-tagged RNA probes generated by: in vitro transcribing a library of fragmented reference genomic DNA that has been ligated to an RNA promoter adaptor, in the presence of an affinity-tagged ribonucleotide; binding the product with a capture agent that is tethered to a substrate in the presence of RNA oligonucleotides that are complementary to the adaptors, thereby capturing the hybridized DNA molecules on the substrate; washing the substrate to remove any unbound DNA molecules; and releasing the captured DNA molecules. A kit for performing the method is also provided.

    Abstract translation: 本文提供了一种在溶液中捕获DNA分子的方法。 该方法可以包括：从包含内源DNA和环境DNA的样品中提取DNA以产生提取的DNA; 将通用接头连接到提取的DNA; 将提取的DNA在溶液中与通过以下方式产生的亲和标记的RNA探针杂交：在亲和标记的核糖核苷酸的存在下体外转录已连接到RNA启动子衔接子的片段参考基因组文库; 在与衔接子互补的RNA寡核苷酸的存在下将产物与捕获剂结合，所述捕获剂被束缚于底物，从而在底物上捕获杂交的DNA分子; 洗涤底物以除去任何未结合的DNA分子; 并释放所捕获的DNA分子。 还提供了用于执行该方法的套件。

    Nucleic acid purification
    66.
    发明授权
    Nucleic acid purification 有权
    核酸纯化

    公开(公告)号：US09206469B2

    公开(公告)日：2015-12-08

    申请号：US13943986

    申请日：2013-07-17

    Applicant: ZYMO RESEARCH CORPORATION

    Inventor： Stanislav Forman ,  Xiyu Jia

    IPC: C12Q1/68 ,  C12N1/08 ,  C12N15/10 ,  C12N15/63

    CPC classification number: C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

    Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, a method is provided for nucleic acid purification from biological samples, such as whole blood samples, extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for of small RNAs (e.g., siRNAs or miRNAs) purification and are amenable to automation.

    Abstract translation: 提供了用于核酸分离的方法和组合物。 在一个实施方案中，提供了用于从不需要相分离或核酸沉淀的基于苯酚的变性溶剂提取的生物样品例如全血样品中进行核酸纯化的方法。 根据本发明的方法也可以用于小RNA（例如siRNA或miRNA）的纯化，并且适于自动化。

    METHODS AND DEVICES FOR PRODUCING BIOMOLECULES
    67.
    发明申请
    METHODS AND DEVICES FOR PRODUCING BIOMOLECULES 审中-公开
    用于生产生物分子的方法和装置

    公开(公告)号：US20150307911A1

    公开(公告)日：2015-10-29

    申请号：US14794048

    申请日：2015-07-08

    Applicant: Boehringer lngelheim RCV GmbH & Co. KG

    Inventor： Jochen URTHALER ,  Christine ASCHER ,  Daniel BUCHELI

    IPC: C12P19/34 ,  C12N15/10

    CPC classification number: C12P19/34 ,  B03D1/028 ,  B03D1/082 ,  B03D1/1462 ,  B03D1/1468 ,  B03D1/1487 ,  B03D1/18 ,  B03D2203/003 ,  C12N15/1003 ,  C12N15/1006

    Abstract: A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).

    Abstract translation: 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。

    NANOSTRUCTURE AND METHODS OF NUCLEIC ACID ISOLATION
    69.
    发明申请
    NANOSTRUCTURE AND METHODS OF NUCLEIC ACID ISOLATION 审中-公开
    纳米结构与核酸分离方法

    公开(公告)号：US20150252407A1

    公开(公告)日：2015-09-10

    申请号：US14201918

    申请日：2014-03-09

    Applicant: Nvigen, Inc.

    Inventor： Aihua Fu ,  Yiguo Shen

    IPC: C12Q1/68 ,  B01J20/02 ,  B01J20/22 ,  B01J20/26 ,  B01J20/28 ,  B01J20/06

    CPC classification number: B01J20/027 ,  B01J20/06 ,  B01J20/22 ,  B01J20/261 ,  B01J20/28009 ,  C12N15/1006 ,  C12N15/1013

    Abstract: A kit comprising a nanostructure comprising at least one core nanoparticle, and a silanization coating on the surface of the core nanoparticle, and a binding buffer comprising a plurality of ingredients at concentration suitable to adjust the concentration of the plurality of ingredients in a solution containing at least one nucleic acid to concentration suitable for binding the nucleic acid through non-hybridization interaction to the nanostructure. A method of using the kit for reversibly binding nucleic acids through non-hybridization based interaction to a nanostructure is also provided.

    Abstract translation: 包含纳米结构的试剂盒，其包含至少一个核心纳米颗粒和在核心纳米颗粒表面上的硅烷化涂层，以及结合缓冲液，其包含多个成分，浓度适于调节多种成分在含有 至少一种浓度适合于通过与纳米结构的非杂交相互作用结合核酸的核酸。 还提供了使用该试剂盒以通过基于非杂交的相互作用与纳米结构可逆结合核酸的方法。

    AGENT AND KIT FOR ISOLATING NUCLEIC ACIDS
    70.
    发明申请
    AGENT AND KIT FOR ISOLATING NUCLEIC ACIDS 审中-公开
    用于分离核酸的试剂和试剂盒

    公开(公告)号：US20150184149A1

    公开(公告)日：2015-07-02

    申请号：US14645174

    申请日：2015-03-11

    Applicant: Pei-Shin JIANG ,  Kun-Chan WU ,  Yu-Ting SU ,  Chia-Yun LIN ,  Siou-Cing SU ,  Yuh-Jiuan LIN

    Inventor： Pei-Shin JIANG ,  Kun-Chan WU ,  Yu-Ting SU ,  Chia-Yun LIN ,  Siou-Cing SU ,  Yuh-Jiuan LIN

    IPC: C12N15/10

    CPC classification number: C12N15/1013 ,  C12N15/1006

    Abstract: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.

    Abstract translation: 提供了分离核酸的方法。 该方法包括提供含有至少一种核酸的生物样品，并在合适的条件下将生物样品与分离剂混合，以在单步骤中分离生物样品中的核酸，其中所述分离剂含有1-40重量％ 的PEG和/或大于30重量％的低分子量醇，盐和洗涤剂。 分离的核酸通过核酸溶解度的变化与固体支持物结合。 另外，本发明还提供了用于分离核酸的分离剂和试剂盒。

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