公开(公告)号：US20150307911A1

公开(公告)日：2015-10-29

申请号：US14794048

申请日：2015-07-08

申请人： Boehringer lngelheim RCV GmbH & Co. KG

发明人： Jochen URTHALER ,  Christine ASCHER ,  Daniel BUCHELI

IPC分类号： C12P19/34 ,  C12N15/10

CPC分类号： C12P19/34 ,  B03D1/028 ,  B03D1/082 ,  B03D1/1462 ,  B03D1/1468 ,  B03D1/1487 ,  B03D1/18 ,  B03D2203/003 ,  C12N15/1003 ,  C12N15/1006

摘要： A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).

摘要翻译： 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。