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公开(公告)号：US10443094B2
公开(公告)日：2019-10-15
申请号：US15419560
申请日：2017-01-30
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper , Peter James Tatnell , Jeffrey Kenneth Horton
IPC: C12Q1/68 , C12Q1/6844 , C12P19/34
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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公开(公告)号：US10350307B2
公开(公告)日：2019-07-16
申请号：US15707074
申请日：2017-09-18
Applicant: General Electric Company
Inventor： Brian Michael Davis , John Richard Nelson , Wei Gao
Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.
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63.发明申请公开(公告)号：US20190083655A1
公开(公告)日：2019-03-21
申请号：US15707074
申请日：2017-09-18
Applicant: General Electric Company
Inventor： Brian Michael Davis , John Richard Nelson , Wei Gao
IPC: A61K48/00
CPC classification number: A61K48/0066 , C12N15/63 , C12N15/67 , C12Q2531/125
Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.
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公开(公告)号：US20190071704A1
公开(公告)日：2019-03-07
申请号：US16041734
申请日：2018-07-20
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US10202636B2
公开(公告)日：2019-02-12
申请号：US14140127
申请日：2013-12-24
Applicant: General Electric Company
Inventor： Bing Li , David Roger Moore , William Christopher Alberts , John Richard Nelson
IPC: C12Q1/68 , C12Q1/6806 , G01N33/543
Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.
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Patent Agency Ranking
公开(公告)号：US20190003940A1
公开(公告)日：2019-01-03
申请号：US15639511
申请日：2017-06-30
Applicant: General Electric Company , University of Akron
Inventor： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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公开(公告)号：US20180345278A1
公开(公告)日：2018-12-06
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: B01L3/00 , C12Q1/02 , G01N21/64 , G01N21/76 , G01N33/554
CPC classification number: C12Q1/02 , C12M41/00 , C12M41/46 , C12Q1/18 , G01N21/6408 , G01N21/6452 , G01N33/4836
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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公开(公告)号：US10100292B2
公开(公告)日：2018-10-16
申请号：US15048624
申请日：2016-02-19
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
IPC: C12Q1/68 , C12N9/22 , C12Q1/6858 , C07K14/00 , C07K1/00 , C12Q1/6844 , C12Q1/6806
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
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公开(公告)号：US09938568B2
公开(公告)日：2018-04-10
申请号：US14933275
申请日：2015-11-05
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
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公开(公告)号：US20170137874A1
公开(公告)日：2017-05-18
申请号：US15419560
申请日：2017-01-30
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper , Peter James Tatnell , Jeffrey Kenneth Horton
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12P19/34 , C12Q2527/107 , C12Q2527/125 , C12Q2527/137 , C12Q2531/119
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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