公开(公告)号：US20180321200A1

公开(公告)日：2018-11-08

申请号：US16035770

申请日：2018-07-16

申请人： SHIMADZU CORPORATION

发明人： Yasuhiro MITO ,  Etsuho KAMATA ,  Hiroshi MIURA ,  Kenichi MISHIMA ,  Toshinobu YANAGISAWA

IPC分类号： G01N30/80 ,  B01D15/08 ,  G01N30/82

CPC分类号： G01N30/80 ,  B01D15/08 ,  G01N30/74 ,  G01N30/82 ,  G01N30/8631 ,  G01N2030/027

摘要： A preparative separation chromatograph system having a column for temporally separating components in a sample and for discharging an eluate fluid with the components, a detector for obtaining an absorbance spectrum of the eluate fluid, and a fraction collector for continuously creating a chromatogram. The system further includes: a peak section determiner; a differential value determiner for calculating a differential spectrum value and for determining whether or not the absolute value of the differential spectrum value is equal to or less than a predetermined value; and a fraction collector controller for controlling the fraction collector so as to fractionate the eluate fluid during a period of time for which it is determined that the chromatogram peak of the target component is present and for which it is also determined the absolute value of the differential spectrum value is equal to or less than the predetermined value.

公开(公告)号：US20180136174A1

公开(公告)日：2018-05-17

申请号：US15574661

申请日：2015-06-01

申请人： Shimadzu Corporation

发明人： Takafumi Nakamura ,  Tadayuki Yamaguchi ,  Yusuke Nagai

IPC分类号： G01N30/38 ,  G01N30/74 ,  G01N30/82 ,  G01N30/84

CPC分类号： G01N30/38 ,  G01N30/74 ,  G01N30/82 ,  G01N30/84 ,  G01N2030/382

摘要： A preparative chromatograph for collecting target components in a sample temporally separated in a column of a chromatograph in respective preparative containers is provided with: a detection unit having a flow cell accommodated in a housing and a detector for detecting a component that passes through the flow cell; a first pipe that connects the column and an inlet end of the flow cell; a flow path switching unit accommodated in the housing and configured to selectively flow the components that passed through the flow cell through a preparative flow path that is a flow path connected to the preparative containers or a waste liquid flow path; and a second pipe accommodated in the housing and connecting an outlet end of the flow cell and the flow path switching unit.

公开(公告)号：US20180120271A1

公开(公告)日：2018-05-03

申请号：US15526506

申请日：2015-11-16

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： Lena Nyholm ,  Lotta Hedkvist ,  Johan Arthursson ,  Eva Cecilia Olsson ,  Henrik Larsson

IPC分类号： G01N30/86 ,  B01D15/24 ,  G01N30/82

CPC分类号： G01N30/8631 ,  B01D15/247 ,  G01N30/82 ,  G01N30/8644

摘要： The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter, identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s) in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.

公开(公告)号：US09470664B2

公开(公告)日：2016-10-18

申请号：US13939731

申请日：2013-07-11

申请人： Waters Technologies Corporation

发明人： Theodore A. Dourdeville ,  Charles Phoebe ,  Rolf Grigat

IPC分类号： G01V3/00 ,  G01N30/04 ,  G01R33/30 ,  G01R33/44 ,  G01N30/82 ,  G01N24/08 ,  G01N30/78 ,  G01N30/84 ,  G01R33/46

CPC分类号： G01N30/04 ,  G01N24/08 ,  G01N24/084 ,  G01N24/085 ,  G01N30/78 ,  G01N30/82 ,  G01N2030/8411 ,  G01R33/302 ,  G01R33/307 ,  G01R33/44 ,  G01R33/46

摘要： A chromatographic interface for sample handling and manipulation between separations or analyses is provided. The interface has an interfacing unit, a sample acquisition unit, and a sample trapping unit. The sample acquisition unit includes an inflow selector valve, an outflow selector valve, and one or more collection loops. The sample trapping unit includes a trap in fluid communication with the loop and having a stationary phase. The stationary phase can trap an analyte of interest from a fraction of the chromatographic sample separation flow. The tapping unit also has a scavenging gas source and a vacuum source; both can be used to substantially dry the stationary phase.

摘要翻译： 提供了色谱界面，用于分离或分析之间的样品处理和操作。 接口具有接口单元，采样单元和采样单元。 样品采集单元包括流入选择阀，流出选择阀和一个或多个收集回路。 样品捕集单元包括与环流体连通并具有固定相的阱。 固定相可从一部分色谱样品分离流中捕获感兴趣的分析物。 攻丝单元还具有清除气源和真空源; 两者都可用于基本干燥固定相。

公开(公告)号：US09314764B2

公开(公告)日：2016-04-19

申请号：US13188337

申请日：2011-07-21

申请人： Robert Hess ,  John Linton ,  Tanya S. Kanigan ,  Colin Brenan ,  Can Ozbal

发明人： Robert Hess ,  John Linton ,  Tanya S. Kanigan ,  Colin Brenan ,  Can Ozbal

IPC分类号： G01N1/10 ,  B01J19/00 ,  B01L3/02 ,  B01L3/00 ,  G01N30/46 ,  B01L99/00 ,  C40B60/14 ,  G01N30/60 ,  G01N30/82 ,  G01N30/84

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L99/00 ,  B01L2200/0657 ,  B01L2300/0845 ,  C12M23/12 ,  C40B60/14 ,  G01N30/466 ,  G01N30/6091 ,  G01N30/6095 ,  G01N30/82 ,  G01N2030/8417

摘要： The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

摘要翻译： 本发明的特征在于制造具有高密度阵列通孔的装置或“压板”的方法，以及清洁和翻新压板表面的方法。 本发明还涉及制备化学，生物化学和生物化合物的高密度阵列的方法，其具有优于常规的较低密度阵列的许多优点。 本发明包括许多物理，化学或生物转化可以在装置的每个可寻址通孔内串联或并行地实现的方法。 此外，本发明包括分析阵列的内容物的方法，包括测定样品的物理性质。

公开(公告)号：US08877051B2

公开(公告)日：2014-11-04

申请号：US11490499

申请日：2006-07-21

申请人： Kimber D. Fogelman ,  Edwin E. Wikfors ,  Rui Chen

发明人： Kimber D. Fogelman ,  Edwin E. Wikfors ,  Rui Chen

IPC分类号： G01N30/82

CPC分类号： G01N30/82

摘要： A system and process for chromatography that uses a chromatography column to separate components from a sample received into the column in a mobile phase flowstream and places a second chromatography column between a detector and a collection system that compensates the timing of fraction collection for a delay caused by processing the collection signals generated by the detector. The device and process of the preferred and alternative embodiments add a delay into the flowstream of a chromatographic system, such as LC, HPLC, and SFC. Sample fractions are collected from sample component concentration peaks based upon the chromatographic elution of the sample components.

摘要翻译： 用于色谱柱的系统和方法，其使用色谱柱将来自在流动相流中流入柱的样品中分离组分，并将第二色谱柱置于检测器和收集系统之间，以补偿引起的延迟的馏分收集时间 通过处理由检测器产生的收集信号。 优选和替代实施方案的装置和方法对色谱系统的流动流如LC，HPLC和SFC增加延迟。 基于样品组分的色谱洗脱，从样品组分浓度峰中收集样品级分。

公开(公告)号：US08752418B2

公开(公告)日：2014-06-17

申请号：US13376924

申请日：2010-06-04

申请人： Hakan Frojdh

发明人： Hakan Frojdh

IPC分类号： G01N1/00 ,  G01N30/82 ,  G01N35/10 ,  G01N30/84

CPC分类号： G01N30/82 ,  G01N35/1016 ,  G01N2030/8411 ,  G01N2035/102

摘要： A fraction collector comprising a liquid holding means (31) wherein it further comprises a fluid front control arrangement (43) arranged for locating the fluid front at a dispensing nozzle (5) and to control the liquid holding means (31) to keep the fluid front at a predetermined position.

摘要翻译： 一种包括液体保持装置（31）的馏分收集器，其中其还包括流体前部控制装置（43），其布置成用于将流体前部定位在分配喷嘴（5）处并且控制液体保持装置（31）以保持流体 在预定位置前面。

公开(公告)号：US20080164210A1

公开(公告)日：2008-07-10

申请号：US11696609

申请日：2007-04-04

申请人： Nicholas DeMarco

发明人： Nicholas DeMarco

IPC分类号： G01N30/82

CPC分类号： G01N30/80 ,  B01L9/06 ,  B01L2300/022 ,  B01L2300/0609 ,  G01N2035/00742

摘要： An apparatus to perform a chromatography process includes a fraction collection system with a rack adapted to support at least one receptacle and a reader coupled to the fraction collection system. The reader is operable to detect a first identification coupled to the rack. The apparatus also includes a chromatography system connected to the fraction collection system to supply a fraction to the receptacle, and a RFID system. The RFID system communicates with the reader and generates data identifying a first location of the receptacle with respect to the rack and a second location of the rack with respect to the fraction collection system.

摘要翻译： 执行色谱过程的装置包括具有适于支撑至少一个容器的架的馏分收集系统和耦合到馏分收集系统的读取器。 读取器可操作以检测耦合到机架的第一标识。 该装置还包括连接到馏分收集系统以向容器提供分数的色谱系统和RFID系统。 RFID系统与阅读器进行通信，并产生识别插座相对于机架的第一位置和相对于分数收集系统的机架的第二位置的数据。

公开(公告)号：US6106710A

公开(公告)日：2000-08-22

申请号：US393595

申请日：1999-09-10

申请人： Steven M. Fischer ,  Glen F. Ingle

发明人： Steven M. Fischer ,  Glen F. Ingle

IPC分类号： G01N30/72 ,  G01N30/82 ,  G01N30/84 ,  B01D15/08

CPC分类号： G01N30/82 ,  G01N30/7233

摘要： A fraction collection system for liquid chromatography includes a conduit, which directs a sample eluent from an LC column through a flow splitter to a destructive analytical detector such as a mass spectrometer to analyze sample components, and to a fraction collector. A nondestructive detector such as a photodetector is positioned near the fraction collector to detect arrival of sample components. Optionally, a nondestructive detector such as a photodetector is situated upstream from the splitter, to detect passing sample components. Time intervals between detection of a calibrant or a sample component at any two of the detectors provide for automatable identification of selected sample components at the sample collector.

公开(公告)号：US6090280A

公开(公告)日：2000-07-18

申请号：US294168

申请日：1999-04-19

申请人： James A. Connelly ,  Linda O'Brien ,  Sue Xiuqing Zhang ,  Ning Zhao

发明人： James A. Connelly ,  Linda O'Brien ,  Sue Xiuqing Zhang ,  Ning Zhao

IPC分类号： G01N30/72 ,  G01N30/74 ,  G01N30/78 ,  G01N30/82 ,  G01N30/86 ,  G01N35/00 ,  G01N35/08 ,  B01D15/08

CPC分类号： G01N30/82 ,  G01N30/8679 ,  B01J2219/00707 ,  G01N30/72 ,  G01N30/78 ,  G01N30/8631 ,  G01N35/08

摘要： A method and apparatus for high-throughput identity confirmation, quantitation and purity determination of a compound of interest in a mixture of compounds is disclosed. According to the method, a chemical mixture, such as may be obtained from a chemical library, is processed to obtain a stream having a time-varying composition, such as by using a high pressure liquid chromatograph. The stream is split into two portions or streams for substantially contemporaneous detection in a compound-identifying device, such as a mass spectrograph, and in a compound-quantitating device, such as an evaporative light scattering detector. A peak in a chromatogram obtained from the compound-quantitating device is identified as the compound of interest by cross-correlation, via elution time, with chromatographic results obtained from the compound-identifying device. Once the peak is identified as corresponding to the compound of interest, the corresponding peak area is used for quantitation and purity determination.

摘要翻译： 公开了一种用于化合物混合物中感兴趣的化合物的高通量身份确认，定量和纯度测定的方法和装置。 根据该方法，可以从化学文库获得化学混合物，例如通过使用高压液相色谱仪，得到具有时变组成的流。 在化合物识别装置（例如质谱仪）和化合物定量装置（例如蒸发光散射检测器）中，该流被分成两部分或物流，用于基本同时检测。 从化合物定量装置获得的色谱图中的峰通过相互相关，通过洗脱时间，由化合物识别装置获得的色谱结果鉴定为感兴趣的化合物。 一旦峰被鉴定为与感兴趣的化合物相对应，则相应的峰面积用于定量和纯度测定。