公开(公告)号：US20230213502A1

公开(公告)日：2023-07-06

申请号：US18148350

申请日：2022-12-29

Applicant: SomaLogic Operating Co., Inc.

Inventor： Dom ZICHI ,  Allison WEISS ,  David SEGHETTI ,  Kathryn JENKO

IPC: G01N33/53 ,  C12N15/10

CPC classification number: G01N33/5308 ,  C12N15/1048

Abstract: Methods of detecting and quantifying target molecules, such as proteins, in a biological sample are provided. The disclosed methods include capturing target molecules with aptamers, replacing the aptamers with aptamer identification sequences, and then sequencing the aptamer identification sequences using next-generation sequencing techniques.

公开(公告)号：US20230213471A1

公开(公告)日：2023-07-06

申请号：US18065118

申请日：2022-12-13

Applicant: Yi XIAO ,  Yingzhu LIU ,  Juan CANOURA

Inventor： Yi XIAO ,  Yingzhu LIU ,  Juan CANOURA

IPC: G01N27/327 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N27/3276 ,  G01N27/3277 ,  G01N33/5308 ,  G01N33/5438

Abstract: The subject invention provides methods for fabricating electrochemical aptamer-based (E-AB) sensors with enhanced sensitivity, signal-to-noise ratios, LOD, and improved stability and reproducibility. The subject invention also provides methods for aptamer immobilization on the surface of the electrode, which favors sufficient spacing between aptamers at the microscale to achieve optimal target recognition, folding, and signal transduction. The E-AB sensors of the subject invention provide superior sensing regardless of the sequence or structure of the bound aptamers or the physiochemical properties of the target.

公开(公告)号：US20230203473A1

公开(公告)日：2023-06-29

申请号：US18061273

申请日：2022-12-02

Applicant: University Health Network ,  Sinai Health System

Inventor： Daniel DINIZ DE CARVALHO ,  Shu Yi SHEN ,  Rajat SINGHANIA ,  Scott Victor BRATMAN ,  Ankur RAVINARAYANA CHAKRAVARTHY

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6804 ,  G01N33/53

CPC classification number: C12N15/1003 ,  C12N15/1093 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6804 ,  G01N33/5308 ,  C12Q1/6886

Abstract: There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.

公开(公告)号：US11685922B2

公开(公告)日：2023-06-27

申请号：US16916305

申请日：2020-06-30

Applicant: Oxford Nanopore Technologies PLC

Inventor： Daniel John Turner ,  Daniel George Fordham ,  Roger Charles Gill ,  Clive Gavin Brown ,  Stuart Reid ,  James Anthony Clarke ,  James White

IPC: G01N33/00 ,  G01N33/543 ,  G01N33/53 ,  C12N15/115 ,  C07H21/04

CPC classification number: G01N33/54386 ,  C12N15/115 ,  G01N33/5308 ,  G01N2333/36 ,  G01N2333/49 ,  G01N2333/705 ,  G01N2333/96463

Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

公开(公告)号：US11684546B2

公开(公告)日：2023-06-27

申请号：US17717854

申请日：2022-04-11

Applicant: SiO2 Medical Products, Inc.

Inventor： Christopher Weikart ,  Becky L. Clark ,  Adam Stevenson ,  John T. Felts

IPC: A61J1/14 ,  A61J1/00 ,  C23C16/02 ,  C23C16/04 ,  C23C16/40 ,  C23C16/455 ,  G01N27/22 ,  G01N33/53 ,  G01N33/543 ,  G01N33/74 ,  A61J1/03 ,  A61J1/05 ,  A61L31/04 ,  A61L31/08 ,  A61L31/14 ,  C23C16/50 ,  A61L31/16 ,  A61M5/31 ,  B65D25/14

CPC classification number: A61J1/1468 ,  A61J1/00 ,  A61J1/035 ,  A61J1/05 ,  A61L31/04 ,  A61L31/08 ,  A61L31/088 ,  A61L31/143 ,  A61L31/16 ,  A61M5/3129 ,  B65D25/14 ,  C23C16/029 ,  C23C16/045 ,  C23C16/401 ,  C23C16/45523 ,  C23C16/50 ,  G01N27/227 ,  G01N33/5308 ,  G01N33/5438 ,  G01N33/74 ,  A61L2420/02 ,  A61L2420/08 ,  A61M2005/3131 ,  A61M2205/0238 ,  G01N2333/62 ,  G01N2800/042

Abstract: An article or vessel is described including a vessel surface and a coating set comprising at least one tie coating, at least one barrier coating, and at least one pH protective coating. For example, the coating set can comprise a tie coating, a barrier coating, a pH protective coating and a second barrier coating; and in the presence of a fluid composition, the fluid contacting surface is the barrier coating or layer. The respective coatings can be applied by PECVD of a polysiloxane precursor. Such vessels can have a coated interior portion containing a fluid with a pH of 4 to 8. The barrier coating prevents oxygen from penetrating into the thermoplastic vessel, and the tie coating and pH protective coating together protect the barrier layer from the contents of the vessel. The second barrier coating is comparable to glass surface if needed.

公开(公告)号：US11656222B2

公开(公告)日：2023-05-23

申请号：US16833904

申请日：2020-03-30

Applicant: HON HAI PRECISION INDUSTRY CO., LTD.

Inventor： Hsin-Hua Lin ,  Wei-Chih Chang ,  Po-Li Shih ,  Chao-Chun Yang ,  I-Min Lu

IPC: G01N33/53 ,  G01N29/22 ,  G01N29/036

CPC classification number: G01N33/5308 ,  G01N29/036 ,  G01N29/22

Abstract: A sensor using ultrasound to detect presence and nature of analyte includes an ultrasonic element and a receptor thereon. The ultrasonic element includes a first electrode, a second electrode facing and spaced apart from the first electrode, an insulating layer between the first electrode and the second electrode, and a vibrating film between the insulating layer and the first electrode. The vibrating film carries the first electrode. A cavity is formed between the vibrating film and the insulating layer. The receptor is on a side of the first electrode away from the second electrode. The receptor can combine with a target substance in a test analyte. When the first electrode and the second electrode are applied with different voltages, certain ultrasound frequencies are generated as the vibrating film vibrates, and the presence and weight of different target substances are indicated by the changes in resonance.

公开(公告)号：US20230152257A1

公开(公告)日：2023-05-18

申请号：US16649697

申请日：2018-09-25

Applicant: SKYHAWK THERAPEUTICS, INC.

Inventor： Kathleen MCCARTHY ,  Michael LUZZIO

IPC: G01N24/08 ,  C12N15/11 ,  C07H21/04 ,  G01N33/53 ,  C12Q1/6816 ,  C12P19/34

CPC classification number: G01N24/088 ,  C12N15/11 ,  C07H21/04 ,  G01N33/5308 ,  C12Q1/6816 ,  C12P19/34

Abstract: Provided herein are structure-based screening platforms and methods to identify small molecules that can bind polynucleotides and/or complexes formed by polynucleotides and proteins. Structure-based screening platforms and methods to characterize interactions of small molecules with polynucleotides and/or with complexes formed by polynucleotides and proteins are also provided herein. Methods and compositions to identify small molecules that can bind polynucleotides and/or polynucleotide-protein complexes involved in RNA splicing are also provided herein.

公开(公告)号：US20190185926A1

公开(公告)日：2019-06-20

申请号：US16192239

申请日：2018-11-15

Applicant: Pacific Biosciences of California, Inc.

Inventor： Jonas Korlach ,  Jeffrey Wegener

IPC: C12Q1/6869 ,  G01N33/53 ,  C07H19/10 ,  C12Q1/6818 ,  C12Q1/6874

CPC classification number: C12Q1/6869 ,  C07H19/10 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/5308

Abstract: Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.

公开(公告)号：US20190090491A1

公开(公告)日：2019-03-28

申请号：US15228759

申请日：2016-08-04

Applicant: AMERICAN UNIVERSITY IN CAIRO (AUC)

Inventor： Hassan Mohamed El-Said AZZAZY ,  Sherif Mohamed SHAWKY ABDUO ,  Kamel Abdelmenem Mohamed EID ,  Bassem Samy Shenouda GUIRGIS

IPC: A01N59/16 ,  C12Q1/6816 ,  G01N33/53 ,  B22F9/20 ,  B22F1/00 ,  C12Q1/70 ,  G01N33/553 ,  G01N33/552 ,  G01N33/58

CPC classification number: A01N59/16 ,  B22F1/0003 ,  B22F9/20 ,  C12Q1/6816 ,  C12Q1/70 ,  G01N33/5308 ,  G01N33/552 ,  G01N33/553 ,  G01N33/587 ,  Y10T436/143333 ,  C12Q2523/31 ,  C12Q2563/155 ,  C12Q2565/628

Abstract: A gold nanoparticle-based assay for the detection of a target molecule, such as Hepatitis C Virus (HCV) RNA in serum samples, that uses positively charged gold nanoparticles (AuNPs) in solution based format. The assay has been tested on 74 serum clinical samples suspected of containing HCV RNA, with 48 and 38 positive and negative samples respectively. The developed assay has a specificity and sensitivity of 96.5% and 92.6% respectively. The results obtained were confirmed by Real-Time PCR, and a concordance of 100% for the negative samples and 89% for the positive samples has been obtained between the Real-Time PCR and the developed AuNPs based assay. Also, a purification method for the HCV RNA has been developed using HCV RNA specific probe conjugated to homemade silica nanoparticles. These silica nanoparticles have been synthesized by modified Stober method. This purification method enhanced the specificity of the developed AuNPs assay. The method can detect a target molecule, such as HCV RNA in serum, by employing modified silica nanoparticles to capture the target from a biological sample followed by detection of the captured target molecule using positively charged AuNPs. The assay is simple, cheap, sensitive and specific. Another aspect of the invention is anisotropic silver nanoparticles and methods of their use.

公开(公告)号：US20190077832A1

公开(公告)日：2019-03-14

申请号：US16196812

申请日：2018-11-20

Applicant: Immatics Biotechnologies GmbH

Inventor： Hans-Georg Rammensee ,  Juliane Stickel ,  Daniel Johannes Kowalewski ,  Stefan Stevanovic ,  Simon Walz

IPC: C07K7/06 ,  C07K14/705 ,  G01N33/574 ,  G01N33/53 ,  A61K38/00

CPC classification number: C07K7/06 ,  A61K35/17 ,  A61K38/00 ,  A61K39/0011 ,  A61K2039/5158 ,  C07K7/08 ,  C07K14/705 ,  C07K16/00 ,  C07K16/30 ,  C12N5/0636 ,  C12N2800/00 ,  G01N33/5308 ,  G01N33/574

Abstract: The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer, in particular myeloma. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.