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公开(公告)号：US12215384B2
公开(公告)日：2025-02-04
申请号：US16304077
申请日：2017-05-25
Applicant: Oxford Nanopore Technologies PLC
Inventor： Richard Alexander Gutierrez , Andrew John Heron , James White
IPC: C12Q1/6869
Abstract: The invention relates to a new method of characterising a target polynucleotide using a pore. The method involves controlling the formation of secondary structure by the target polynucleotide after the polynucleotide has moved through the pore.
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公开(公告)号：US20240158848A1
公开(公告)日：2024-05-16
申请号：US18527900
申请日：2023-12-04
Applicant: Oxford Nanopore Technologies PLC
Inventor： James Anthony Clarke , James White , John Milton , Clive Gavin Brown
IPC: C12Q1/6869 , B82Y15/00 , G01N27/447 , G01N33/487
CPC classification number: C12Q1/6869 , B82Y15/00 , G01N27/44743 , G01N33/48721
Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
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公开(公告)号：US11959135B2
公开(公告)日：2024-04-16
申请号：US17064633
申请日：2020-10-07
Applicant: Oxford Nanopore Technologies PLC
Inventor： James Anthony Clarke , James White , John Milton , Clive Gavin Brown
IPC: C12Q1/6869 , B82Y15/00 , G01N27/447 , G01N33/487
CPC classification number: C12Q1/6869 , B82Y15/00 , G01N27/44743 , G01N33/48721
Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
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公开(公告)号：US20240117337A1
公开(公告)日：2024-04-11
申请号：US18340824
申请日：2023-06-23
Applicant: Oxford Nanopore Technologies PLC
Inventor： James White
CPC classification number: C12N15/1065 , C07H21/04 , C12N15/1031 , C12N15/111 , C12N15/66 , C12N2310/122
Abstract: A method of amplifying a target polynucleotide, comprising: providing a template polynucleotide comprising a 5′ hairpin, a target polynucleotide and a 3′ hairpin, wherein the 5′ hairpin comprises one or more non-canonical nucleotides and contacting the template polynucleotide with a polymerase and canonical nucleotides, wherein the polymerase extends, using the canonical nucleotides, the target polynucleotide from its 3′ end to form a first extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin; and the polymerase extends the first extended polynucleotide from its 3′ end to form a second extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin.
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公开(公告)号：US20240076729A9
公开(公告)日：2024-03-07
申请号：US18010826
申请日：2021-06-18
Applicant: Oxford Nanopore Technologies PLC
Inventor： Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC: C12Q1/6869
CPC classification number: C12Q1/6869
Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
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Patent Agency Ranking
公开(公告)号：US11725205B2
公开(公告)日：2023-08-15
申请号：US16412346
申请日：2019-05-14
Applicant: Oxford Nanopore Technologies PLC
Inventor： James White
CPC classification number: C12N15/1065 , C07H21/04 , C12N15/1031 , C12N15/111 , C12N15/66 , C12N2310/122
Abstract: A method of amplifying a target polynucleotide, comprising: providing a template polynucleotide comprising a 5′ hairpin, a target polynucleotide and a 3′ hairpin, wherein the 5′ hairpin comprises one or more non-canonical nucleotides; and contacting the template polynucleotide with a polymerase and canonical nucleotides, wherein the polymerase extends, using the canonical nucleotides, the target polynucleotide from its 3′ end to form a first extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin; and the polymerase extends the first extended polynucleotide from its 3′ end to form a second extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin.
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公开(公告)号：US20230212535A1
公开(公告)日：2023-07-06
申请号：US18047019
申请日：2022-10-17
Applicant: Oxford Nanopore Technologies PLC
Inventor： Mark Bruce , Andrew John Heron , Ruth Moysey , Szabolcs Soeroes , Elizabeth Jayne Wallace , James White
IPC: C12N9/14 , C12N9/90 , C12Q1/6869
CPC classification number: C12N9/14 , C12N9/90 , C12Q1/6869 , C12Q2565/631 , C12Y306/04012
Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：US11685922B2
公开(公告)日：2023-06-27
申请号：US16916305
申请日：2020-06-30
Applicant: Oxford Nanopore Technologies PLC
Inventor： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC: G01N33/00 , G01N33/543 , G01N33/53 , C12N15/115 , C07H21/04
CPC classification number: G01N33/54386 , C12N15/115 , G01N33/5308 , G01N2333/36 , G01N2333/49 , G01N2333/705 , G01N2333/96463
Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
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公开(公告)号：US20230158503A1
公开(公告)日：2023-05-25
申请号：US17989814
申请日：2022-11-18
Inventor： Matthew Holden , James White , Andrew John Heron , James Anthony Clarke , Jason Robert Hyde , Benjamin James Hadwen , Sally Anderson
IPC: B01L3/00 , C12Q1/6869 , G01N33/487
CPC classification number: B01L3/502792 , C12Q1/6869 , G01N33/48721 , B01L2300/165 , B01L2300/0645 , B01L2400/0427
Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
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公开(公告)号：US11525125B2
公开(公告)日：2022-12-13
申请号：US16893332
申请日：2020-06-04
Applicant: Oxford Nanopore Technologies PLC
Inventor： Mark John Bruce , Andrew John Heron , Ruth Moysey , Szabolcs Soeroes , Elizabeth Jayne Wallace , James White
IPC: C12N9/14 , C12N9/90 , C12Q1/6869
Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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