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    DEVICE AND METHOD FOR EXTRACTING INDIVIDUAL PICOLITER DROPLETS FROM MICROFLUIDIC EMULSIONS FOR FURTHER ANALYSIS AND SCALE-UP
    51.
    发明申请
    DEVICE AND METHOD FOR EXTRACTING INDIVIDUAL PICOLITER DROPLETS FROM MICROFLUIDIC EMULSIONS FOR FURTHER ANALYSIS AND SCALE-UP 审中-公开
    从微流化乳液中提取个体PICOLITER DROPLETS进一步分析和定标的装置和方法

    公开(公告)号：US20160129443A1

    公开(公告)日：2016-05-12

    申请号：US14937672

    申请日：2015-11-10

    申请人： Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie - Hans-Knöll-Institut

    发明人： Miguel Tovar ,  Emerson ZANG ,  Thomas WEBER

    IPC分类号： B01L3/00

    CPC分类号： B01L3/502761 ,  B01L3/0268 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2400/0424 ,  B01L2400/0487 ,  C12M25/01 ,  G01N15/1484 ,  G01N35/1011 ,  G01N2015/149 ,  G01N2035/1034 ,  G01N2035/1044

    摘要： The disclosure relates to a method and a system for the isolation of picoliter droplets from a continuous stream of carrier fluid in a microfluidic system. In particular the disclosure relates to a system that comprises a sorter, a detector and a collector. The sorter serves for a selection of picoliter droplets with a desired content based upon an optical signal obtained from the picoliter droplets. The selected picoliter droplets are guided towards the detector that comprises a detection channel that is arranged perpendicular to a light beam. By means of a photodetector that is aligned with the optical axis of the light beam the passage of the picoliter droplets can be detected. The collector comprises multiple wells and the detection of the passage of the picoliter droplets results in a relative movement of the detector and a collector such that the picoliter droplet is isolated by depositing it into one of said wells. The disclosure moreover relates to a method that uses such a system to isolate individual picoliter droplets. In particular the disclosure relates to a method that allows for an analysis and/or further processing of the content of the picoliter droplets that have been isolated from a microfluidic system by depositing them into a well.

    摘要翻译： 本公开涉及一种用于从微流体系统中的载流体的连续流中分离皮升液滴的方法和系统。 特别地，本公开涉及一种包括分拣机，检测器和收集器的系统。 分选机基于从皮升液滴获得的光学信号，用于选择具有所需内容的皮升液滴。 所选择的皮升液滴被引导到包括垂直于光束布置的检测通道的检测器。 通过与光束的光轴对准的光电检测器，可以检测到皮升液滴的通过。 收集器包括多个孔，并且检测皮升液滴的通过导致检测器和收集器的相对运动，使得通过将皮升液滴沉积到其中一个孔中来分离皮升液滴。 本发明还涉及使用这种系统来分离单个皮升液滴的方法。 特别地，本公开涉及一种允许通过将它们沉积到井中而从微流体系统分离的皮升液滴的内容物进行分析和/或进一步处理的方法。

    Method of purging trapped gas from a system fluid contained in an actuation valve
    55.
    发明授权
    Method of purging trapped gas from a system fluid contained in an actuation valve 失效
    从包含在致动阀中的系统流体清除被捕获的气体的方法

    公开(公告)号：US07169616B2

    公开(公告)日：2007-01-30

    申请号：US10353824

    申请日：2003-01-27

    申请人： James E. Johnson ,  Neil R. Picha ,  Craig M. Storms ,  David A. Martin

    发明人： James E. Johnson ,  Neil R. Picha ,  Craig M. Storms ,  David A. Martin

    IPC分类号： G01N1/10 ,  B01L3/02 ,  F16T1/42

    CPC分类号： B01L3/021 ,  B01L3/0268 ,  B01L2200/143 ,  B01L2200/148 ,  B01L2300/14 ,  B01L2400/02 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0633 ,  B01L2400/0666 ,  G01N35/00594 ,  G01N35/00712 ,  G01N35/1002 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00237 ,  G01N2035/1041 ,  G01N2035/1044 ,  Y10T137/3003 ,  Y10T436/15 ,  Y10T436/2575

    摘要： An apparatus and method for delivering repetitive, precision, low volume liquid dispensing from a dispensing orifice of a non-contact liquid dispensing apparatus. An elongated communication passageway of the dispensing apparatus is defined by interior walls having one end in fluid communication with a system fluid reservoir and an opposite end terminating at the dispensing orifice. A system fluid is placed in the communication passageway extending substantially continuously from the system fluid reservoir to the dispensing orifice. A relatively small volume of gaseous fluid is aspirated through the dispensing orifice, and into the communication passageway in a manner such that the gaseous fluid extends substantially continuously across the transverse cross-sectional dimension of the communication passageway. Subsequently, a dispensing liquid is aspirated through the dispensing orifice and into the communication passageway in a manner such that the relatively small volume of aspirated gaseous fluid forms a minute, unitary air gap fully enclosed between the interior walls of the communication passageway and a liquid interface between the system fluid and the dispensing liquid contained in the communication passageway. This minute air gap substantially prevents dispersion and dilution therebetween at the liquid interface. To effect dispensing, a rapid pressure pulse with a predetermined pulse width is applied to the system fluid upstream from the minute air gap, causing the pressure pulse to traverse the minute air gap to the dispensing liquid without substantial fluid compression of the minute air gap. This enables substantially accurate, relatively small volume, non-contact liquid dispensing of the dispensing liquid from the dispensing orifice.

    摘要翻译： 一种用于从非接触式液体分配装置的分配孔输送重复，精确，低体积的液体分配的装置和方法。 分配装置的细长的连通通道由具有与系统流体储存器流体连通的一端和终止于分配孔的相对端的内壁限定。 系统流体被放置在从系统流体储存器到分配孔基本上连续延伸的连通通道中。 通过分配孔吸入相对小体积的气态流体，并以这样的方式进入连通通道，使得气态流体基本上连续地延伸穿过连通通道的横截面尺寸。 随后，分配液体通过分配孔吸入并进入连通通道，使得相对较小体积的吸入气态流体形成完全封闭在连通通道的内壁之间的微小整体空气间隙和液体界面 在系统流体和包含在连通通道中的分配液体之间。 这种微小气隙基本上防止了液体界面处的分散和稀释。 为了实现分配，将具有预定脉冲宽度的快速压力脉冲施加到从微小空气间隙上游的系统流体，使得压力脉冲穿过分配液体的微小空气间隙，而不会明显地流体压缩微小气隙。 这使得能够从分配孔口实现分配液体的基本上精确的，相当小体积的非接触液体分配。

    Device for parallel metering of liquids
    57.
    发明授权
    Device for parallel metering of liquids 有权
    用于平行计量液体的装置

    公开(公告)号：US07036917B2

    公开(公告)日：2006-05-02

    申请号：US10728111

    申请日：2003-12-05

    申请人： Birgit Müller-Chorus ,  Ralf-Peter Peters ,  Holger Bartos

    发明人： Birgit Müller-Chorus ,  Ralf-Peter Peters ,  Holger Bartos

    IPC分类号： B41J2/17

    CPC分类号： B01L3/0241 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/06 ,  B41J2/17566 ,  G01N35/1002 ,  G01N35/1065 ,  G01N35/1072 ,  G01N2035/1041 ,  G01N2035/1044

    摘要： A device for parallel metering of a liquid with the following features: the device has a first body; the first body has a main channel, secondary channels, one inlet and outlets; the main channel is connected to the inlet; the secondary channels are connected to one outlet at a time; the secondary channels are connected to the main channel; the device has at least one first chamber with a first pressure medium; the first body has means for transferring transfer means pressure surges of the pressure medium from a first chamber to the secondary channels; each transfer means is connected to a secondary channel; and at least one means for preventing a fluidic connection between the secondary channels and the chamber is assigned to the transfer means.

    摘要翻译： 一种用于平行计量液体的装置，具有以下特征：该装置具有第一主体; 第一机体有主要通道，二级通道，一个入口和出口; 主通道连接到入口; 次级通道一次连接到一个出口; 次级通道连接到主通道; 所述装置具有至少一个具有第一压力介质的第一室; 第一主体具有用于将压力介质的压力波动从第一室传递到第二通道的装置; 每个传送装置连接到辅助信道; 并且至少一个用于防止二次通道和腔室之间的流体连接的装置被分配给传送装置。

    Device for parallel metering of liquids
    59.
    发明申请
    Device for parallel metering of liquids 有权
    用于平行计量液体的装置

    公开(公告)号：US20040183869A1

    公开(公告)日：2004-09-23

    申请号：US10728111

    申请日：2003-12-05

    发明人： Birgit Muller-Chorus ,  Ralf-Peter Peters ,  Holger Bartos

    IPC分类号： B41J002/17

    CPC分类号： B01L3/0241 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/06 ,  B41J2/17566 ,  G01N35/1002 ,  G01N35/1065 ,  G01N35/1072 ,  G01N2035/1041 ,  G01N2035/1044

    摘要： Device for parallel metering of a liquid with the following features: the device has a first body (20a, 20b); the first body (20a, 20b) has a main channel (8), secondary channels (9, 10, 11, 12), one inlet (1) and outlets (6); the main channel (8) is connected to the inlet (1); the secondary channels (9, 10, 111, 12) are connected to one outlet at a time; the secondary channels (9, 10, 11, 12) are connected to the main channel (8); the device has at least one first chamber with a first pressure medium; the first body (20a, 20b) has means for transferring (transfer means 2) pressure surges of the pressure medium from a first chamber to the secondary channels; each transfer means (2) is connected to a secondary channel (9, 10, 11, 12); at least one means (21) for preventing a fluidic connection between the secondary channels (9, 10, 11, 12) and the chamber is assigned to the transfer means (2).

    摘要翻译： 用于平行计量液体的装置具有以下特征：所述装置具有第一主体（20a，20b）; 第一主体（20a，20b）具有主通道（8），次通道（9,10,11,12），一个入口（1）和出口（6）; 主通道（8）连接到入口（1）; 次级通道（9,10,111,12）一次连接到一个出口; 第二通道（9,10,11,12）连接到主通道（8）; 所述装置具有至少一个具有第一压力介质的第一室; 第一主体（20a，20b）具有将压力介质的压力从第一室转移到第二通道的装置（传送装置2） 每个传送装置（2）连接到辅助信道（9,10,11,12）; 至少一个用于防止第二通道（9,10,11,12）和腔室之间的流体连接的装置（21）被分配给传送装置（2）。