知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

80 records (took 0.043 seconds)

    Luminescent metal complexes and associated technology
    51.
    发明授权
    Luminescent metal complexes and associated technology 有权
    发光金属配合物及相关技术

    公开(公告)号：US09193746B2

    公开(公告)日：2015-11-24

    申请号：US11952867

    申请日：2007-12-07

    Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

    Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

    IPC: G01N21/64 ,  C07F15/00 ,  C09B57/10 ,  G01N33/68

    CPC classification number: C07F15/0053 ,  C09B57/10 ,  G01N33/6839 ,  G01N2458/40

    Abstract: Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.

    Abstract translation: 本文公开了发光金属络合物，其制备和/或设计方法，使用方法及相关技术。 发光金属络合物可用于各种应用，例如染色，检测和/或鉴定，例如物质，例如聚（氨基酸）。 进一步作为示例，发光金属络合物可用于染色，检测和/或鉴定与各种环境中的任何环境相关的聚（氨基酸），例如凝胶或凝胶基质，例如任何与 例如，SDS-PAGE，例如表面环境，例如与western印迹相关的任何例如和/或类似物。 本文还公开了包含发光金属络合物的组合物，溶液和试剂盒。

    METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY
    53.
    发明申请
    METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY 有权
    使用染料与核酸染色或检测及相关技术相结合的方法

    公开(公告)号：US20130167309A1

    公开(公告)日：2013-07-04

    申请号：US13541313

    申请日：2012-07-03

    Applicant: Fei Mao ,  Wai-Yee Leung

    Inventor： Fei Mao ,  Wai-Yee Leung

    IPC: G01N1/30

    CPC classification number: C09B15/00 ,  C09B11/28 ,  C09B23/04 ,  C12Q1/6818 ,  G01N1/30 ,  G01N21/6486 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/301

    Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

    Abstract translation: 提供了使用染料和相关技术的方法。 可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。 这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。 可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。 在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

    Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology
    58.
    发明申请
    Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology 有权
    使用染料与核酸染色或检测及相关技术相结合的方法

    公开(公告)号：US20100323453A1

    公开(公告)日：2010-12-23

    申请号：US12854436

    申请日：2010-08-11

    Applicant: Fei Mao ,  Wai-Yee Leung

    Inventor： Fei Mao ,  Wai-Yee Leung

    IPC: G01N33/52

    CPC classification number: C09B15/00 ,  C09B11/28 ,  C09B23/04 ,  C12Q1/6818 ,  G01N1/30 ,  G01N21/6486 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/301

    Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

    Abstract translation: 提供了使用染料和相关技术的方法。 可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。 这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。 可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。 在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

    SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS
    60.
    发明申请
    SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS 审中-公开
    融合蛋白中的亲和标签的特定标签

    公开(公告)号：US20090081722A1

    公开(公告)日：2009-03-26

    申请号：US12117689

    申请日：2008-05-08

    Applicant: Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    Inventor： Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

    IPC: G01N1/30 ,  C07F5/02 ,  C07D311/06

    CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

    Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

Patent Agency Ranking